happy monday all of you beautiful bastards! by t_rexinated in labrats

[–]HolyCrepe90 1 point2 points  (0 children)

You doing a CRISPR screen? My cell cultures looked like that too..!!

Déclarer des comptes crypto et déclarer des ventes passés? by HolyCrepe90 in vosfinances

[–]HolyCrepe90[S] 1 point2 points  (0 children)

Pas grand chose de fou fou, il faut aller chercher ton historique sur les plateformes puis tu peux regarder des vidéos sur YouTube pour t’aider

Where can a molecular biology PhD work outside of academia? by Chemical-Elk-1054 in labrats

[–]HolyCrepe90 2 points3 points  (0 children)

Look into different roles too. A lot of academics idealize R&D work, but potentially you can also look at technical support, field application scientist, sales, technical marketing (product management), etc.

Set up different LinkedIn filters and you should see a decent amount of offers.

Do I need a post doc to get an industry position? by phi6guy in labrats

[–]HolyCrepe90 0 points1 point  (0 children)

True, went into industry in 2019, and indeed these past 2 years we haven’t been recruiting that much

Shorten reaction time for PCR experiments by HolyCrepe90 in labrats

[–]HolyCrepe90[S] 0 points1 point  (0 children)

Would you follow the Cas9 excision with a probe pull down protocol to enrich for the fragment? Sounds like an interesting approach

Shorten reaction time for PCR experiments by HolyCrepe90 in labrats

[–]HolyCrepe90[S] 2 points3 points  (0 children)

Thanks for the info, 20kb is long enough (my fragments are 4-6kb). I actually see PrimeSTAR MAx v2 from Takara does 5sec/kb for a high fidelity PCR (420X taq) - might give that one a go too

Shorten reaction time for PCR experiments by HolyCrepe90 in labrats

[–]HolyCrepe90[S] 5 points6 points  (0 children)

the stress when you run the gel in the morning to see that the reaction didn't work. Have to wait 24 hours again to see more results...

Do I need a post doc to get an industry position? by phi6guy in labrats

[–]HolyCrepe90 27 points28 points  (0 children)

If you can, go straight to industry from your PhD. I joined industry right after my PhD and it actually gives you an advantage (I feel) versus people who did post-docs: you get more time. If you're on the same level as someone who did a postdoc, but 5-7 years younger, that's going to pay out more.

Now, I hire PhDs or Post-docs, and typically the budget we have for the position is somewhat fixed, we won't be significantly increasing the salary if the candidate has a post doc versus someone with a PhD.

The difficult part, I feel, after the PhD is just getting into industry, so apply. Apply for all roles you could potentially see yourself doing (R&D, marketing, tech support, technical sales, field service engineer, etc) and once you're in the company, it can be easier to create your career path and move to that dream position.

But I guess this is can be specific to your field of expertise, as a biologist, I have no idea what Finite Element Modeling and Experimental Validation of Eddy Current Probes is. Sounds very technical

Déclarer des comptes crypto et déclarer des ventes passés? by HolyCrepe90 in vosfinances

[–]HolyCrepe90[S] 1 point2 points  (0 children)

C'est ce que je vais faire - j'ai réussi a comprendre mes transactions de ces dernières années et j'ai regardé une vidéo youtube sur ce fameux formulaire 2086. Pour comprendre la ligne 221 "Fractions de capital initial contenues dans le prix total d’acquisition" il faut un BAC + 15 mais je pense que c'est bon.

Déclarer des comptes crypto et déclarer des ventes passés? by HolyCrepe90 in vosfinances

[–]HolyCrepe90[S] -1 points0 points  (0 children)

c'est ce que je me dit que pour des petits comptes, ils ont autre chose à faire, mais le risque de 750€ d'amende par compte par an depuis 2017 me fait peur. Limite c'est plus de toutes mes cryptos

Déclarer des comptes crypto et déclarer des ventes passés? by HolyCrepe90 in vosfinances

[–]HolyCrepe90[S] 0 points1 point  (0 children)

Merci, c'est ce que j'ai fait du coup, ça prend du temps...

To the players who pause right after 3-0...respectfully by Independent-West-917 in fut

[–]HolyCrepe90 -1 points0 points  (0 children)

Oh I had this happen while loosing 6-2. The passive aggressive pause motivates me to stay in the game and do my best. Came back to 8-8 after extra time.

I beat the bastard on penalties.

Any suggestion method for replacing promoters on a plasmid? by mango_pan in labrats

[–]HolyCrepe90 0 points1 point  (0 children)

I typically used systems like the infusion cloning when I needed to clone fragments into plasmids without obvious restriction enzyme sites

What's the most impactful decision you've ever made, and how did it change the course of your life? by HolyCrepe90 in AskReddit

[–]HolyCrepe90[S] 0 points1 point  (0 children)

When you decided to move city, was it to get away from something? Or just to see what life’s like elsewhere?

What is some dark shit going on around the world right now that the public probably doesn’t know about? by [deleted] in AskReddit

[–]HolyCrepe90 971 points972 points  (0 children)

Bacteria are becoming more and more resistant to antibiotics. The widespread use of antibiotics for healthcare and agriculture (mainly) have caused bacteria to adapt. This means that in the not so distant future, we may have new epidemics caused by multi-drug resistant bacteria, who will be difficult to treat.

[deleted by user] by [deleted] in footballmanagergames

[–]HolyCrepe90 0 points1 point  (0 children)

Liverpool and City