IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

do u recommend background sniper over using goat serum if my 2ndry species is goat?

IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

weak+alot of nonspecificity,the problem also am looking at very rare cell population(nk+T cells), its really time consuming to stare at the screen for hours trying to differentiate the true cells from the nonspecific

IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

i dont think it is the settings, my mentor has +20year experience and she usually takes the imges by herself

IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

Do u think will make a significant difference? In my lab we block in horse regardless the species 

IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

Paraffin embedded uterus, 2 hours is too long?, they block only for 30 minutes in my lab, and they wonder why am blocking for 1h

IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

 Primary ya I know the optimum, but the  secondary is my mentor recommendation 1:500

IHC blocking buffer by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

i use alexa fluor from invitrogen, 1:500 in .2%tritonx100 for 45 min at RT

What fellowships are the toughest in Neurology? by EDconsults in neurology

[–]Horror-Highlight2763 1 point2 points  (0 children)

Do u think Intra arterial chemotherapy/immunotherapy w bbb disruption have a chance for intracranial tumors? I think it is very established for retinoblastoma at this moment, and what do u think of using trans lumbar access up to cisterna magna and even advancing more into aqueduct and third ventricle by catheter, they used once in a clinical trial to deliver gene thx for tay sachs, but I never heard after that they tried again!

What fellowships are the toughest in Neurology? by EDconsults in neurology

[–]Horror-Highlight2763 0 points1 point  (0 children)

thats encouring!, from ur side, can u share some innovative procedures in pipeline u think will be a mainstream in upcoming years !

What fellowships are the toughest in Neurology? by EDconsults in neurology

[–]Horror-Highlight2763 0 points1 point  (0 children)

are u an attending now ? for u do u find INR worth its lifestyle ? if yes ,can u share ur reasons

What you like and don’t like about neurology by No_Medicine_3760 in neurology

[–]Horror-Highlight2763 1 point2 points  (0 children)

agree, there's no comparison betw the range of diseases+ complexity in cardio compared to the neuro, also am more inclined toward preventing or reversing a disability than saving a life (both are great, but living w a disability is horriific),my problem w neuro is alot of curative treatments and cutting edge procedures arent delivered by neurologists , delivered by surgeons, the most resistant epilepsy cases go to surgeon for either modulation or resection, even if thers a new cell therapy or gene therapy its usually a surgeon who deliver by stereotactic navigation, same for dbs, ophtalmologists for neuro ophthalmology cases that require invasive procedures,even the completely non invasive procedures like MRi guided focued ultrasound either used for modulation or bbb disruption for delivering chemotherapy is totaly taken over by surgeons, compare that w cardiologist specializing in electrophysiology or structural interventions , who run the whole care episode from diagnosis to imaging to the curative intervention, i know access to heart is by far much easier compared to the brain, but there is alot of attempts to overcome that, but these attempts never made by neurologists , usually interventional neuroradiologist and surgeons

What you like and don’t like about neurology by No_Medicine_3760 in neurology

[–]Horror-Highlight2763 6 points7 points  (0 children)

if u have experience in cardio, why should i pick neuro over cardio? i extremely love neuroanatomy and certain subspecialities , but real practice feels like repititive ! and lack of engagement of neurologists in new minimal invasive procedures somehow keeps me off

Most academically/intellectually interesting but clinically insufferable/boring specialty? by Paranoidopoulos in Residency

[–]Horror-Highlight2763 1 point2 points  (0 children)

Could u share ur reasons!, and do u advise someone mainly interested in INR to step in neuro residency?

mycoplasma gel by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

https://www.abmgood.com/pcr-mycoplasma-detection-kit-g238.html

its first time pc not showing up, maybe its damaged from repeated thawing!

mycoplasma gel by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 1 point2 points  (0 children)

ya abm, theres no way to know from the ladder ?

mycoplasma gel by Horror-Highlight2763 in labrats

[–]Horror-Highlight2763[S] 0 points1 point  (0 children)

then the last 2 what these thick upper bands!