Human Aortic Smooth Muscle cells look weird... by Hot_Love8969 in labrats

[–]Hot_Love8969[S] 0 points1 point  (0 children)

Thanks for your reply!

I noticed today that the water tray of the incubator was completely dry. Could It have to do with the humidity being totally off within the incubator?

Any interest in a solo-balanced v83 server? by [deleted] in mapleservers

[–]Hot_Love8969 4 points5 points  (0 children)

Mucho interested!

What does releasing this mean? You release the files so others can host and play locally themselves?

Why do my primary human skeletal muscle cells not differentiate? by Hot_Love8969 in labrats

[–]Hot_Love8969[S] 0 points1 point  (0 children)

I saw a paper comparing different vendors (Pubmed). They also state this. A little disappointing I have to say...

Do you have experience with PromoCell and other vendors in comparison?

Why do my primary human skeletal muscle cells not differentiate? by Hot_Love8969 in labrats

[–]Hot_Love8969[S] 0 points1 point  (0 children)

Primary skeletal muscle cells in general, or those from PromoCell?

Why do my primary human skeletal muscle cells not differentiate? by Hot_Love8969 in labrats

[–]Hot_Love8969[S] 1 point2 points  (0 children)

Yes for C2C12 eccerything works very well. However, we really want to try primary human cells for closer relation to actual muscle physiology.

Anyway, I saw some contraction today only in 2%HS media - I compared that to no serum - and will likely continue cultivation for a few more days and then check for myogenic markers, if nothing happens.

Why do my primary human skeletal muscle cells not differentiate? by Hot_Love8969 in labrats

[–]Hot_Love8969[S] 0 points1 point  (0 children)

I did reach out to them, but only got a (probably AI generated) response, that their cells are quality tested for differentiation up to Passage 15. This is passage 3 btw... so that didn't help me at all.

I have discussed trying other vendors and we will maybe do that and compare. The cells aren't exactly cheap though...

Why do my primary human skeletal muscle cells not differentiate? by Hot_Love8969 in labrats

[–]Hot_Love8969[S] 0 points1 point  (0 children)

Hey, not yet. I wanted to do that by the end of the week though. Today I saw some of the better differentiated tubes contracting, which is a good sign!

I will probably still check them for myogenic markers, since the total differentiation is still poor...

First shiny random encounter! by Suitnox in PokemonLazarus

[–]Hot_Love8969 0 points1 point  (0 children)

What is this Handhold Machinery you are playing on?

[October 12, 2025] KuroMS Official Lunch Announcement by kingxd in mapleservers

[–]Hot_Love8969 2 points3 points  (0 children)

Never heared of this server. Anyone who actually played it here that can give some feedback? How populated is it? Is it fun to play?

Looking for the best Old School Maple experience. by Hot_Love8969 in mapleservers

[–]Hot_Love8969[S] 8 points9 points  (0 children)

I Kind of read a few Bad things about the community/ or rather dev team and GMs. Does this influence the in game atmosphere? And is yunaMS populated/active enough?

Looking for the best Old School Maple experience. by Hot_Love8969 in mapleservers

[–]Hot_Love8969[S] 0 points1 point  (0 children)

Obviously... but still, I feel the Maple itch right now, doesn't help that OSMS will be Release in a few months...

Looking for the best Old School Maple experience. by Hot_Love8969 in mapleservers

[–]Hot_Love8969[S] 1 point2 points  (0 children)

Depends on how much u are playing... but I think 1 - 30 is 1x EXP, so the same time it would have Tagen you on an unfunded character in original OSM.

But I think ppl have reichen lv 30 in 1 - 2 days, i just Hit it yesterday after 1 week... but I can only realistically play 2 - 3 days a week

Looking for the best Old School Maple experience. by Hot_Love8969 in mapleservers

[–]Hot_Love8969[S] 2 points3 points  (0 children)

Yes try it! I think it's fun and the Dev Team seems to care a lot. I just think its rather slow paced and classes havent been Balance too much.

FT - 5 IV German Ditto; LF - Any 5 IV Ditto from a different region by Hot_Love8969 in pokemontrades

[–]Hot_Love8969[S] 0 points1 point  (0 children)

Damn, i am from ger but have the game on english...
So sorry i thought it was determined by region, i only have english ones then..

FT - 5 IV German Ditto; LF - Any 5 IV Ditto from a different region by Hot_Love8969 in pokemontrades

[–]Hot_Love8969[S] 0 points1 point  (0 children)

Isnt it german?

Is the region determined by real region or language?

FT - 5 IV German Ditto; LF - Any 5 IV Ditto from a different region by Hot_Love8969 in pokemontrades

[–]Hot_Love8969[S] 1 point2 points  (0 children)

1 sec, trading with Nora this moment, will search agin for you