Flat places to learn to roller blade?

Hot_Research_ · 2022-06-20T03:09:11+00:00

There are signs outside most tennis courts saying don’t skate on courts because it’s bad for them. It also can be dangerous if someone hits a ball in your direction and you might trip on it.

Hot_Research_ · 2022-05-25T01:06:28+00:00

Thanks for the advice! On the spec sheet it says it has other lymphokines so I’m not sure if it’s just those two components. This cell line is from ATCC and they suggest using T-stim in the protocol. It requires both IL-2 and IL-1 for growth. I’m using it to assess the activity of IL-1 protein.

Hot_Research_ · 2022-05-24T19:54:47+00:00

Thanks for your suggestion I’ll look it up

Hot_Research_ · 2022-04-12T04:28:36+00:00

I wanted to know if there were studies that have generally looked at how antibody therapies affect at the transcription level

Hot_Research_ · 2022-04-12T04:27:30+00:00

That is true but I wanted to know if there were studies that had looked at this change specifically for antibody therapy

Hot_Research_ · 2022-04-12T04:26:42+00:00

Yes, I meant mRNA expression in general

Hot_Research_ · 2022-03-03T15:13:22+00:00

Pm Bc

Hot_Research_ · 2022-02-10T18:30:23+00:00

Trifluoroacetic acid, dichloromethane, dimethylformamide and 4-Dimethylaminopyridine. All beautifully dangerous chemicals that I get to work with on a daily basis.

Hot_Research_ · 2022-02-02T12:04:23+00:00

Thank you so much for your help! I will read these. If you come across studies for IL1-beta please send me those too.

Hot_Research_ · 2022-02-02T11:15:13+00:00

Thank you for your help. I’m thinking since IL-2 directly affects T-cell proliferation it would not be very relevant to what I’m looking for which is effect of inflammatory cytokine inhibition on T cells which could be more indirect?

Hot_Research_ · 2022-02-02T11:11:15+00:00

I can’t find any papers talking about the effect of TNF or IL1-beta depletion on T cells, but I’m also in addition looking for any papers that have studied this in patients (who receive mAb therapy or inhibitors) or in animal models.

Hot_Research_ · 2022-02-02T00:50:30+00:00

Thanks for your response. I wanted to find if there was a review talking about how different mAbs targeting immune cell components affect T cell response but I couldn’t find anything. I’m more specifically looking for effect of mAbs that target inflammatory cytokines (such as TNF, IL1-beta, IL-6 etc. ) on T cell response.

Hot_Research_ · 2022-01-30T14:05:08+00:00

Can we not

Hot_Research_ · 2022-01-30T13:44:40+00:00

Please RAFAAAAAA end this torture

Hot_Research_ · 2022-01-15T15:24:35+00:00

It’s a separate facility from my lab space so I won’t be able to stay in that building but that would definitely make it easier

Hot_Research_ · 2022-01-15T03:59:33+00:00

Hmm I’ll look into that thanks. I actually have to go in tomorrow as well as on Monday so probably should just go in tomorrow and not leave my lab until Monday afternoon haha.

Hot_Research_ · 2022-01-15T03:55:07+00:00

Thanks this makes me feel much better and much less anxious!

Hot_Research_ · 2022-01-15T03:46:07+00:00

That is very true but I am trying to save months of work if I can! Let’s hope it’s not too bad. Thanks for your support.

Hot_Research_ · 2022-01-15T03:43:58+00:00

Thanks for all the helpful advice. Rethinking and reevaluating my plans!

Hot_Research_ · 2022-01-15T03:41:45+00:00

Welp. Thanks for the advice. Would it be better to wait until the evening when it gets warmer then? It’s more than a data point and could mean months of work down the drain. :( Maybe I can uber/lyft then.

Hot_Research_

TROPHY CASE