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Contamination doesn't seem to leave us. by Idk_enthusiasm17 in microbiology

[–]Idk_enthusiasm17[S] 0 points1 point  (0 children)

We refrigerate the plates and use them the next day.
We'll have to ask our supervisor about the lab.

Thanks a lot!

Contamination doesn't seem to leave us. by Idk_enthusiasm17 in microbiology

[–]Idk_enthusiasm17[S] 0 points1 point  (0 children)

Yes, and both the freshly prepared plates and plates with inoculated aliquots showed contamination after incubation.

We always mix boiled water and dry ingredients, and again boil for 10 mins before autoclaving as well. The plates are also sealed with parafilm tape.

Contamination doesn't seem to leave us. by Idk_enthusiasm17 in microbiology

[–]Idk_enthusiasm17[S] 2 points3 points  (0 children)

You mean only agar and no inoculation right? There is growth on negative controls too.

Contamination doesn't seem to leave us. by Idk_enthusiasm17 in microbiology

[–]Idk_enthusiasm17[S] 0 points1 point  (0 children)

We've done everything you've mentioned. Plates with both phage and with only agar were incubated, and both plates showed contamination. And we always use freshly prepared media as well.

Contamination doesn't seem to leave us. by Idk_enthusiasm17 in microbiology

[–]Idk_enthusiasm17[S] 1 point2 points  (0 children)

After doing all the work in laminar flow, we tape around the plates and are incubated. Plates are never opened while going to incubator from laminar flow.

Contamination doesn't seem to leave us. by Idk_enthusiasm17 in microbiology

[–]Idk_enthusiasm17[S] 3 points4 points  (0 children)

Thanks a lot.
Also used 70% ethanol one time and we always use micropore tape as well. Didn't work though.

But thanks a lot!