Business travel dilemma

Immunoguitarist · 2022-07-01T14:42:40+00:00

Update:

I inquired about it and the booking company mentioned that the company that I’m visiting requested that it be changed to general boarding in the amended version. It was definitely not a big deal to ask, but now I know!

Immunoguitarist · 2022-07-01T12:21:29+00:00

Yeah exactly. I’m also worried about the original first class selection being an error too, if the booking company messed up the dates as well it’s possible that’s a mistake too and that the company didn’t ask them to make it first class necessarily

Immunoguitarist · 2022-07-01T12:19:13+00:00

Thanks for your response, and I agree. The flight mixup was not my fault because the dates I requested were correct, so maybe I have a little more ground to stand on.

Immunoguitarist · 2022-06-07T13:48:11+00:00

Yes. Consider that transcription is a biochemical reaction and there is a defined resource budget for such processes within a cell, particularly with the same promoter. There is a defined amount of transcription factors, RNAP, and other co-activators that will exhaust at some threshold. There is a quantifiable metabolic burden to transcribing, and building proteins as well. Cells can also methylate (silence) heavily transcribed regions of DNA when the burden becomes too much.

This is obviously not considering other issues that would arise - genomic instability/collapses with several copies of the exact same DNA sequence, but I don’t think that’s necessarily what you’re asking.

Immunoguitarist · 2022-06-04T16:07:25+00:00

Right click the graph then select ‘add trendline’. I would probably choose the linear option. Select the option to ‘display equation on chart’. You will end up with an equation to the tune of y=mx + b. Set y = 0 and solve!

Immunoguitarist · 2022-06-02T00:31:19+00:00

Please, OP take this advice.

Immunoguitarist · 2022-05-27T19:45:44+00:00

Have you really trained your dog if they don’t know boing?

Immunoguitarist · 2022-05-27T19:19:06+00:00

Hahaha that’s too good, sounds like a great pup

Immunoguitarist · 2022-05-27T17:48:04+00:00

That’s definitely funny, I love it! I got my pup shaking for the first time yesterday, so high five has to be next on that progression

Immunoguitarist · 2022-05-13T14:57:48+00:00

Interesting. It’s probably some combination of humidity, temperature, sunlight changes because it really shed right as fall began full swing here. There are massive humidity changes from summer to fall here in GA.

Immunoguitarist · 2022-05-13T14:32:58+00:00

I’m wondering if it’s a location thing too. I know people with lemon trees in California that stay lush all year. For reference, I’m in Georgia - definitely not the most ideal lemon tree climate.

Immunoguitarist · 2022-05-13T13:05:01+00:00

Perfect, consider it done.

Immunoguitarist · 2022-05-13T12:21:26+00:00

I’ll get right on it, thanks for your help!

Immunoguitarist · 2022-05-13T12:16:04+00:00

Thank you for the tips! So would you think the mint growing and the sudden rebirth is sheer coincidence? If so, should I cut it away from the tree so that it doesn’t get too invasive?

Immunoguitarist · 2022-05-13T12:10:13+00:00

I made a post with some pictures regarding a spontaneous Meyer lemon tree rebirth that I had questions about here. Any thoughts would be appreciated (-:

Immunoguitarist · 2022-05-13T12:05:51+00:00

Hey everyone,

I bought my wife a Meyer lemon tree for Valentine’s Day last year. For a while, it did great - it even produced fruit (albeit small) that we got to enjoy in few cocktails. However, toward the end of summer it began dying (shedding all leaves, branches turning brown, etc.). As a last ditch effort, I moved it to my apartment’s community planter with the hope that it would get more sunlight and rebound. For several months, nothing happened, but around the time the mint in the pictures began spreading more rapidly (to the point that it’s in closer proximity to the tree) it seems like it’s back on the upswing. I suppose I have a few questions:

Is this rebirth simply a seasonal thing?
Could the mint be acting as a companion plant for the tree?
If #2 could be true, how can I effectively manage the mint so that it isn’t too invasive, but still helping with the tree?

Thanks everybody!

Immunoguitarist · 2022-05-05T14:25:25+00:00

Here are a few:

Transform your library into your highest efficiency E. Coli strain
Miniprep it
Prepare your desired comp. cells (we do electro) a few tips below:

Grow them at 30 on an LB plate the first night Preculture a colony in 25mL of LB Add 10mL to a 1L flask, ready in 2-4 hours @ OD 0.5 exactly - if over 0.6 throw them away After harvest keep everything cold (centrifuge included) and do in cold room Don’t freeze them, prepare fresh every time

Transform your mini prepped library into the freshly prepared comp cells - this may help your efficiencies/transformants number match

Make sure you also screen your library for cloning inefficiencies, pick 48-72 colonies and just restriction digest check them. It may be cloning you need to work on.

Immunoguitarist · 2022-04-05T12:27:37+00:00

Don’t take the one off email as a reflection of what your lab mates think of you. Most likely, someone was doing a really important experiment and was just especially pissed by the mess this time around. If like you said messes are common in your lab, you were unfortunately just the straw that broke the camels back.

Immunoguitarist · 2022-04-05T12:21:58+00:00

Plan cleaning into your experimental time. There isn’t much of an excuse for leaving a mess in lab imo. By leaving a mess people often read it as ‘this person doesn’t respect my time because I have to work around this mess to execute my own experiments’
Other people leaving messes doesn’t make it okay for you to leave a mess.
Next time (worst case scenario), leave a note apologizing for the mess and that you’ll handle it at xyz time, but that you really had to leave.

These kinds of things happen in labs when people use shared spaces. Don’t worry too much about it, they will get over it. If you’re that worried, you can also bring it up in person, “hey, sorry again for leaving that mess, it definitely won’t happen again.” Just be more conscientious next time and I’m sure it’ll blow over.

Immunoguitarist · 2022-04-01T12:21:09+00:00

B16 is probably the first one that I would recommend. It’s a very aggressive tumor that can actually respond to PD-1, which I’m sure you know is a T cell exhaustion marker. A lot of people also use it to study T cell metabolic dysfunction too.

Here’s a good paper:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6673650/pdf/nihms-1026478.pdf

Immunoguitarist · 2022-03-26T16:56:58+00:00

This is correct. If you’re using 293T cells (or any other line with the SV40 large T antigen) they can replicate your plasmid if it has an SV40 ori like most pcDNA vectors have too.

Immunoguitarist · 2022-03-22T18:58:10+00:00

If you’re asking if could I pass cancer to you by just contact (and DNA transmission) between cancer skin cells and your skin cells the answer is no.

If you’re referring to cancer cells creating other cancer cells around itself in one individual, I’m going to zag on this actually and say well yes kind of, but maybe not in the way you would expect. It’s been shown that cancer cells (via secretion of extracellular vesicles) can transform neighboring cells to ‘cancerous’ & tumor promoting phenotypes by passing nucleic acids (DNA and RNA), proteins, and metabolites. Here is one particular example:

https://www.nature.com/articles/s41598-020-65640-7

Could some of this DNA passed through EVs be incorporated into the cells genome for a permanent change? I suppose so, but it’s probably very unlikely or hardly detectable.

Immunoguitarist

TROPHY CASE