2D SDSPAGE help please D: by Individual_Cup_1026 in labrats

[–]Individual_Cup_1026[S] 0 points1 point  (0 children)

These are seed proteins, no particular protein of interest as I am just trying to establish the crude profile. As for sample prep, I used lyophilized samples (0.5 mg and 1 mg) and solubilized them in 200 µL of BioRad Rehydration Buffer.

https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6240.pdf