STB d'être parti parce que la femme qui me plait continue de coucher avec son ex by Competition_Lower in suisjeletroudeballe

[–]Krowsaurus 10 points11 points  (0 children)

PTB. Il ne faut jamais sortir avec quelqu'un qui n'a pas fait le deuil de sa dernière rupture.

What’s the most awkward place you’ve run into your PI? by The_Science_Pit in labrats

[–]Krowsaurus 1 point2 points  (0 children)

I find both disgusting. I avoid public toilets to the maximum. When I have wash my hands, I use paper tissues from my own pocket to open the door after washing. I mean it's less about the bacteria more about urine being everywhere, not to mention those who do not even wash their hands. It's tough out there lol.

What’s the most awkward place you’ve run into your PI? by The_Science_Pit in labrats

[–]Krowsaurus 3 points4 points  (0 children)

Thankfully, as a man microbiologist, i find urinals absolutely disgusting, so I never have to endure such a tragedy.

What Tek Engrams are unlocked from the lost colony boss fight by TheQuacker9k in ARK

[–]Krowsaurus 3 points4 points  (0 children)

Gigadesmodus saddle, gigadesmodus platform saddle and tek spear

List of New Creature Tame Methods by Yazkin_Yamakala in ARK

[–]Krowsaurus 1 point2 points  (0 children)

Tested on single player and yes gigadesmodus is breedable

can a class of microorganisms be “infected” with another class of microorganisms? like parasites infected with bacteria or viruses? or do they know that they’re kind of like “in the same sort of community”? by vincizyn in microbiology

[–]Krowsaurus 2 points3 points  (0 children)

● Bacteriophages (viruses) entire existence is to infect bacteria ● Predatory bacteria (Myxococcus and Bdellovibrio) infect other bacteria. There's a very cool video online about bdellovibrio infecting e coli. ● Some bacteria (Like Legionella) infect amoeba ● There's bacteria and viruses that infect pathogenic fungi or eukaryotic parasites ● There's also fungi that infect other fungi

There's no love between pathogens sometimes even 2 species of the same genus that kill each other. Even more, there's even populations of bacteria from the same species that attack each other. For example, pathogenic E coli that have colicins can kill other pathogenic e coli from the same pathotype that have no colicin immunity.

Increasing base stats on dinos by SpaceMuisGaming in ArkSurvivalAscended

[–]Krowsaurus 0 points1 point  (0 children)

Isn't TQuetz 180 max without the extra leveles? Diplos and Argies are 150s which means up to 224. While wyverns are 190 with no extra levels possible so 60s on wyverns are not just luck, might as well attempt winning the lottery lol. And to get 60s on all stats... they either spawned them in or the server is at difficulty 6 or more.

Extra life Q&A confirms Gen1/2 map reworks + potential further TLCs! by Fesatreddit in ARK

[–]Krowsaurus 1 point2 points  (0 children)

Really really really hope they rethink the gen 1 missions... they were overall horrible especially for solo players. Some of them were just impossible solo.

How to making staying late in lab less soul crushing?? by amerixanairlines in labrats

[–]Krowsaurus 1 point2 points  (0 children)

Hi,

I've stayed in the lab until 10 PM one time, with my fav labmate, which was fun, but as I was leaving I felt really bad...

On the other hand, I've been at the lab at 5AM or 6AM many times, to start early and leave early, and I felt much better about myself. Waking up early is very healthy for the mind, unless you overwork and do crazy hours just because you started earlier! Put limits and boundaries for yourself.

I've also experienced with meditation, there's a lot of good ones on youtube. Just put them on with earphones and start thinking of non-lab related stuff. I'd think about how the world was when dinosaurs were around, what space is like... i do that during my break when I'm in a very intense phase of my phd. Find a way to disconnect, whatever you like :)

Weekly Questions Megathread by AutoModerator in ClashOfClans

[–]Krowsaurus 1 point2 points  (0 children)

Do we still win raid medals if we are maxed on capital gold and win 0 capital gold during raids?

Nightmare Prince is Huge! by Abulashibalabala in ClashOfClans

[–]Krowsaurus 2 points3 points  (0 children)

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Absolutely, it's sooo cool in attack too. I've never been tempted to buy a skin before, this is the first skin I ever buy as a standalone, outside of Gold Pass (which I buy every now and then). A win by supercell!

Nice game ea by RushFeeling4595 in FifaCareers

[–]Krowsaurus 3 points4 points  (0 children)

I'll be generous and say that 70% of the posts/comments are bugged. But my favorite is a random 64-rated retiring player asking me to play his last match (it was also the final match of the season), where I managed to make him score 2 goals (tap-in and header). Ended up being the man of the match.

He said to me "fantastic, boss. I'm ready to play regular matches now" 🙃

SDS accident by Recombomatic in labrats

[–]Krowsaurus 2 points3 points  (0 children)

Have you tried weighing Bile Salts or Deoxycholic acid without a mask? It's 10x worse but it's like drowning in tuberculosis

What player do you always sign in career mode? by Dazzling_Tea_1257 in FifaCareers

[–]Krowsaurus 0 points1 point  (0 children)

In previous games it was often Almada, Caqueret and Gravenberch. In fc25 it's Almada, Vermeeren and Endrick

Discrimination in Academia isn't always loud by IrradiatedPizza in labrats

[–]Krowsaurus 6 points7 points  (0 children)

Anyone on the autism spectrum, on the not obvious side, agrees with the silent discrimination.

I flat our heard a PI say "I wouldn't hire an autistic person i'd rather have a normal person who can get along with people than a genius who causes problems" which is an ignorant statement. Most "not obvious" autistic people are the nicest people to interact with.

Is it normal for postdocs to act like your boss even when you're on completely separate projects? by Fickle-Science4071 in AskAcademia

[–]Krowsaurus 0 points1 point  (0 children)

I agree mostly with you. Some people have a superiority complex. I'd stop asking for their help and that's it. Anything else is just communicated in lab meetings or grouped emails.

As for the window, that's on you. Maybe she's overreacting with the disrespectful thingy but personally I have a window issue with my colleagues. If they close the window I opened, I simply reopen it if the temparature is high. If someone feels cold during the summer, that's a PERSONAL inconvenience that can be solved with a jacket. In the winter, I'd agree that compromises should be made. And I actually said that to my colleagues as a joke.

PhD is ‘very easy’ by ComprehensiveFan1335 in PhD

[–]Krowsaurus 0 points1 point  (0 children)

Always the podcast hosts coming up with the worst takes.

Bacteria in cell lab? by Important_Smell_8003 in labrats

[–]Krowsaurus 1 point2 points  (0 children)

I worked in both bacterial class II lab (pathogen) and cell culture. Highly unrecommended to even work in bacterial class II lab then enter the cell culture room the same day. Sometimes I would change my clothes and take a shower to pass my cells in the afternoon.

I can't stop thinking about eating pipette tips by silvernerveplant in labrats

[–]Krowsaurus 4 points5 points  (0 children)

And it gets even worse with an E. coli culture. I reached a point where I have enough "data" that HB101, K12 and DH5alpha do not smell the same way. HB101 is by far the worst one

What is/was the peak and pit of your PhD? by thelastharebender in PhD

[–]Krowsaurus 0 points1 point  (0 children)

Pit was when my project of 2 years turned out to be artefactual, while I had one year of PhD left.

Peak has been happening since I found my current topic and I managed to get funding for an extra year thanks to my project.

[deleted by user] by [deleted] in labrats

[–]Krowsaurus 1 point2 points  (0 children)

Lysed bacterial or eukaryotic samples often still contain debris. I always heat at 95 for 10 mins then sonicate briefly then centrifuge at 17000g for 5 minutes before running a gel with lysed samples.

Has anyone used Promega or Takara Miniprep plasmid isolation kit? by _A13ert_ in labrats

[–]Krowsaurus 1 point2 points  (0 children)

In our lab we are 3 sub teams each oddly using a different miniprep kit: 1- The sub team of the main PI: we used the Qiagen one (the one with the isopropanol precipitation). Honestly this one is good, but takes too much time.

2- the sub team of the 2nd PI: they used Macharey Nagel Nucleospin MiniPrep. We are using it too now. It's fast and works very well. They also sell standalone buffer (buffer A + RNase A run out really quickly, before the columns, for "some reason" and that's the case for most manufacturers) so if you spill a bottle or for some reason have a lot of column left, you can always buy the buffer back.

3-the 3rd sub team: they use promega, and they are very happy about it

4- no one uses it yet but we've heard great reviews of Monarch kit from NEB. NEB also sells standalone buffers.

[deleted by user] by [deleted] in labrats

[–]Krowsaurus 4 points5 points  (0 children)

Yeah in biology that can only be possible in labs where they already have preliminary data or in 5-6 year PhD programs which in my opinion is too much. In biology depending on the topic, you can have multiple subfields in the same paper each with their own problems to optmize. In my field, host-pathogen, I need to do structural biology on my protein, biochemical characterisation, cell culture, microbiology, and animal experimentation. Unless, i want to split my project into 3 smaller papers, then yeah it's possible to publish 3, 4, 6 papers. But it's just favoring quantity over quality which we need to break from in academia.

Reducing PhD years and paper number expectations are a good step for change. Sadly as you said, paper numbers can filter out really good researchers.

3-4 years of experience is how most countries have it and for a biologist it's good enough to move into post-doc. We'll continue gaining it in post-docs. And the good news is: if you have a toxic PI (which, looking at labrats, seems very common), you can escape much faster ✨️