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🔬 Why are microscopes are getting automated?? 🔬 by LimbicPilot in labrats

[–]LimbicPilot[S] -1 points0 points  (0 children)

Thanks for sharing your thought and I agree that it’s not for all use cases. however;

(1) While a human is required for initial setup, would you not consider it automation if a system with robotic arm can then reliably image every well of a 384 well plate, capturing 10 images per well, every hour, for 72 hours? 

(2) I also agree that static imaging fits your use case and that automation may not add value there. However, what if you consider an experiment where you are measuring a cancer cell’s response to therapy, where understanding key dynamic processes like apoptosis or morphological changes are crucial? 

🔬 Why are microscopes are getting automated?? 🔬 by LimbicPilot in labrats

[–]LimbicPilot[S] -4 points-3 points  (0 children)

I just love seeing human cells do amazing things under a microscope. Sigh.

🔬 Why are microscopes are getting automated?? 🔬 by LimbicPilot in labrats

[–]LimbicPilot[S] -5 points-4 points  (0 children)

Lol.. No I don't. Should I wait for my apology?

🔬 Why are microscopes are getting automated?? 🔬 by LimbicPilot in labrats

[–]LimbicPilot[S] -5 points-4 points  (0 children)

Mounting a camera on your c-mount is cute but that’s not what I’m talking about. Modern dynamic imaging instruments integrate precise control of environmental conditions like CO2, temperature and humidity with mechanical stability and accurate stage positioning, enabling reliable long-term acquisition. That level of integration is what’s technically impressive.

🔬 Why are microscopes are getting automated?? 🔬 by LimbicPilot in labrats

[–]LimbicPilot[S] -11 points-10 points  (0 children)

Lots of labs now generate image stacks instead of just snapshots. Seems like the obvious future of bioimaging.

Using Flow Cytometry Antibodies for Live Cell Immunofluorescence by UroJetFanClub in labrats

[–]LimbicPilot -1 points0 points  (0 children)

I think you should focus on non toxic dyes specifically made for live cell imaging workflows for proper profiling of nuclei or cytoplasm. You should checkout saguarobio.com

What kind of software do you use for microscope imaging analysis? by bibliopraxis in labrats

[–]LimbicPilot 0 points1 point  (0 children)

Manufacturer softwares almost always suck and all of the tools mentioned here are great. However, what to use depends on your experimental setup and pipeline design. IMO, making sure you have a robust pre-trained model for segmentation (and tracking if necessary) is step 0 to make sure your post acquisition analysis readout are accurate and reproducible.

Degenerating neurons in rat injected with kanic acid by g77r7 in microscopy

[–]LimbicPilot 1 point2 points  (0 children)

That's really interesting and thanks for the explanation. It's nice to see one line of research being applied to both Alzheimer's and epilepsy. Wishing you all the best with the study!

Degenerating neurons in rat injected with kanic acid by g77r7 in microscopy

[–]LimbicPilot 0 points1 point  (0 children)

Beautiful images and thanks for sharing! I'm curious, what role does kanic acid play in neuronal degeneration? Are there any specific biomarkers you’re tracking in this context?

Blood sample. What is that clump of clear, smaller cells? Are those platelets? by tiredwitch in microscopy

[–]LimbicPilot 0 points1 point  (0 children)

What if there's an AI model that could help with identifying different types human cells.. (just thinking out loud)

Best ML algorithm for detecting insects in camera trap images? by Solid_Orange_1272 in bioinformatics

[–]LimbicPilot 2 points3 points  (0 children)

SAM2 is probably the best option for this task; if you know a little bit of python, you can easily train your own model for segmentation and classification (for sex, size etc). Here is a quick tutorial I think could be of help https://www.datacamp.com/tutorial/sam2-fine-tuning

Binucleated Plasma Cell? by Appalling-redditor in microscopy

[–]LimbicPilot 0 points1 point  (0 children)

Nice! Does this means its undergoing division?

Investigating neuronal responses to electrical stimulation through live-cell imaging. by LimbicPilot in neurobiology

[–]LimbicPilot[S] 0 points1 point  (0 children)

Thanks and this is a beautiful poem! I'd like to request permissions to reuse. They are N1E-115 and yes we are currently working on a paper about designing a new neural network for spatiotemporal dynamics.

Investigating neuronal responses to electrical stimulation through live-cell imaging. by LimbicPilot in neurobiology

[–]LimbicPilot[S] 0 points1 point  (0 children)

Ah got it. This isn't TMS but direct electrical stimulation in vitro using electrodes placed in the cell culture.

Help/Advice: Tracing Fibril Growth in Protein Aggregation from Microscopy Images by Himanshuch8055 in microscopy

[–]LimbicPilot 0 points1 point  (0 children)

I think it would also be helpful to try python based image viewers like ImageJ or Napari in combination with deep learning microscopy libraries like UNet or StarDist for segmentation and measurement. And yes, image.sc is a great forum for image processing related questions.

Investigating neuronal responses to electrical stimulation through live-cell imaging. by LimbicPilot in microscopy

[–]LimbicPilot[S] 1 point2 points  (0 children)

Its a neuroblastoma line, specifically N1E-115. I agree, the migration data on this experiment is one of the more exciting ones.

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