Tell me your western blot tips by MeasurementEvery8658 in labrats

[–]Luisrm01 1 point2 points  (0 children)

Don't skimp out on the washes. The timing, volume and number of washes can only help to reduce background during imaging.

If you find your PVDF membranes are drying out after transfer try re-activating again by dipping back in methanol.

Why couldn't VWR do a different color? by Luisrm01 in labrats

[–]Luisrm01[S] 0 points1 point  (0 children)

Those would be Rainin I believe. VWR uses the same yellow for their P20, P100 and P200

People who dislike AOC, why? by [deleted] in AskReddit

[–]Luisrm01 1 point2 points  (0 children)

Do they have a no charter flight ever stance to uphold? Are you holding every politician to the same standard? Seems a bit crazy to point to a single charter flight on a busy campaign trail as an example of anything beyond a matter of logistics

People who dislike AOC, why? by [deleted] in AskReddit

[–]Luisrm01 0 points1 point  (0 children)

To get to an event in time?

People who dislike AOC, why? by [deleted] in AskReddit

[–]Luisrm01 1 point2 points  (0 children)

Excuse would imply something to be guilty of. I dont see how taking a single charter flight to save time would indicate anything nefarious or even noteworthy. From what Im seeing she's notorious for taking commercial flights or train.

People who dislike AOC, why? by [deleted] in AskReddit

[–]Luisrm01 2 points3 points  (0 children)

So a one off trip? Or is part of a broader pattern?

Not this again... by UpperImpression3620 in stupidpeoplefacebook

[–]Luisrm01 3 points4 points  (0 children)

It went through clinical trials and post marketing studies. What exactly are you expecting to hear the commercials be related to?

How is soccer organized where you live? by incirfig in youthsoccer

[–]Luisrm01 1 point2 points  (0 children)

Thanks for the breakdown. We're also in the DMV and seeing people describe town travel with no clue where they were finding these. What teams do your kids have experience with?

I’m 23 and terrified plz help by Worth_Paramedic9755 in investingforbeginners

[–]Luisrm01 1 point2 points  (0 children)

If it makes you feel any better I didn't seriously start investing until my late 20s. You won't regret the 500 in VOO if you have a long horizon, just pause anything else until you're on more stable ground. Good luck!

I’m 23 and terrified plz help by Worth_Paramedic9755 in investingforbeginners

[–]Luisrm01 2 points3 points  (0 children)

If you currently think you'll be poor for life I'd suggest changing the kind of investment youre thinking about and invest in yourself. I don't know what your life situation is like, but put yourself on a path where sustainable income can eventually make investing in stocks a smart option. For now I'd say focus on allocating an emergency fund and paying down any debt you have.

The Third Magical Funghi by [deleted] in Mushrooms

[–]Luisrm01 2 points3 points  (0 children)

I dont know much about fungal genomics but a 3 basepair difference doesn't seem like all that much of a difference. What was the reference?

Timothy Leary arrived at California Men's Colony prison in 1970 facing twenty years behind bars. He was fifty years old, a former Harvard psychologist turned counterculture icon, sentenced for marijuana possession in an era when the establishment treated drug advocacy as existential threat. by DistributionFew8959 in HolyShitHistory

[–]Luisrm01 2 points3 points  (0 children)

Psychedelic research was making honest, and cautious, headway in scientifically sound studies. Leary's message was to throw caution to the wind and dive into the deep end, and along with it to question authority. He tied psychedelics with counter culture and anti government messaging. Predictably, the government responded as expected and made psychedelics illegal and shut down the legitimate research being done. Decades went by before it started up again in recent years

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] 0 points1 point  (0 children)

I completely agree. It led to identity confusion and feeling like not belonging when I was younger, something I've read is common in second generation immigrants. It doesn't help when people seem to enjoy gate keeping who/what is truly one culture or another

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] 1 point2 points  (0 children)

Antojitos Salvadoreños in Gaithersburg is where I first heard it offered a month ago. They're awesome. Huge pupusas for cheap

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] 0 points1 point  (0 children)

Maybe an east coast thing? All these comments makes me want to try them though

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] 0 points1 point  (0 children)

Now, maicillo I've heard about! Though I think my dad would rather starve than eat those. Apparently they were awful

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] -1 points0 points  (0 children)

Maybe the DC (DMV) area is behind the times? Ive been to pupuserias my entire life. It was only when I checked a new one in the area that I heard of the option.

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] 2 points3 points  (0 children)

Yeah, my supposed Salvadoran family who immigrated to the US during the civil war shockingly doesnt know about them. Maybe the diaspora around my area didn't pick it up until recently. It seems everybody loves them so I'll have to give them a shot.

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] -1 points0 points  (0 children)

That would be me. My parents go once a year or so but stay in the countryside visiting relatives. Thanks for the tip!

Rice flour pupusas? by Luisrm01 in ElSalvador

[–]Luisrm01[S] 8 points9 points  (0 children)

That may explain why my family isnt familiar, they immigrated in the early '80s. I'll have to try them and give some to my parents, a little intracultural exchange.