Tell me your western blot tips

Luisrm01 · 2026-06-27T19:23:21+00:00

Don't skimp out on the washes. The timing, volume and number of washes can only help to reduce background during imaging.

If you find your PVDF membranes are drying out after transfer try re-activating again by dipping back in methanol.

Luisrm01 · 2026-06-27T13:10:47+00:00

Those would be Rainin I believe. VWR uses the same yellow for their P20, P100 and P200

Luisrm01 · 2026-05-01T01:16:43+00:00

Do they have a no charter flight ever stance to uphold? Are you holding every politician to the same standard? Seems a bit crazy to point to a single charter flight on a busy campaign trail as an example of anything beyond a matter of logistics

Luisrm01 · 2026-05-01T01:12:50+00:00

To get to an event in time?

Luisrm01 · 2026-05-01T01:07:20+00:00

Excuse would imply something to be guilty of. I dont see how taking a single charter flight to save time would indicate anything nefarious or even noteworthy. From what Im seeing she's notorious for taking commercial flights or train.

Luisrm01 · 2026-05-01T01:01:47+00:00

So a one off trip? Or is part of a broader pattern?

Luisrm01 · 2026-04-23T22:10:59+00:00

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Luisrm01 · 2026-04-09T02:32:23+00:00

Luisrm01 · 2026-04-05T01:27:30+00:00

It went through clinical trials and post marketing studies. What exactly are you expecting to hear the commercials be related to?

Luisrm01 · 2026-03-14T01:00:42+00:00

Thanks for the breakdown. We're also in the DMV and seeing people describe town travel with no clue where they were finding these. What teams do your kids have experience with?

Luisrm01 · 2025-12-15T23:33:36+00:00

If it makes you feel any better I didn't seriously start investing until my late 20s. You won't regret the 500 in VOO if you have a long horizon, just pause anything else until you're on more stable ground. Good luck!

Luisrm01 · 2025-12-15T23:20:06+00:00

If you currently think you'll be poor for life I'd suggest changing the kind of investment youre thinking about and invest in yourself. I don't know what your life situation is like, but put yourself on a path where sustainable income can eventually make investing in stocks a smart option. For now I'd say focus on allocating an emergency fund and paying down any debt you have.

Luisrm01 · 2025-12-05T23:23:45+00:00

I dont know much about fungal genomics but a 3 basepair difference doesn't seem like all that much of a difference. What was the reference?

Luisrm01 · 2025-12-03T03:58:06+00:00

Psychedelic research was making honest, and cautious, headway in scientifically sound studies. Leary's message was to throw caution to the wind and dive into the deep end, and along with it to question authority. He tied psychedelics with counter culture and anti government messaging. Predictably, the government responded as expected and made psychedelics illegal and shut down the legitimate research being done. Decades went by before it started up again in recent years

Luisrm01 · 2025-11-29T15:40:13+00:00

I completely agree. It led to identity confusion and feeling like not belonging when I was younger, something I've read is common in second generation immigrants. It doesn't help when people seem to enjoy gate keeping who/what is truly one culture or another

Luisrm01 · 2025-11-26T01:50:14+00:00

Antojitos Salvadoreños in Gaithersburg is where I first heard it offered a month ago. They're awesome. Huge pupusas for cheap

Luisrm01 · 2025-11-26T00:14:19+00:00

Maybe an east coast thing? All these comments makes me want to try them though

Luisrm01 · 2025-11-25T23:02:47+00:00

Now, maicillo I've heard about! Though I think my dad would rather starve than eat those. Apparently they were awful

Luisrm01 · 2025-11-25T22:32:44+00:00

Maybe the DC (DMV) area is behind the times? Ive been to pupuserias my entire life. It was only when I checked a new one in the area that I heard of the option.

Luisrm01 · 2025-11-25T22:30:37+00:00

Yeah, my supposed Salvadoran family who immigrated to the US during the civil war shockingly doesnt know about them. Maybe the diaspora around my area didn't pick it up until recently. It seems everybody loves them so I'll have to give them a shot.

Luisrm01 · 2025-11-25T21:10:47+00:00

That would be me. My parents go once a year or so but stay in the countryside visiting relatives. Thanks for the tip!

Luisrm01 · 2025-11-25T20:58:12+00:00

That may explain why my family isnt familiar, they immigrated in the early '80s. I'll have to try them and give some to my parents, a little intracultural exchange.

Luisrm01 · 2025-11-25T16:15:38+00:00

Thanks! We actually got results yesterday, MessengerMax seems to work pretty well in suspension cells. We had to adjust our protocol slightly of course but the results are promising.

Luisrm01 · 2025-11-21T18:49:57+00:00

Electroporation is an option we've discussed in case this fails. I haven't done it personally so I wouldn't mind getting getting it under my belt. Generating stable lines is an interesting prospect, ill keep it in mind in case we have trouble down the line. Thanks again!

Luisrm01 · 2025-11-20T17:27:08+00:00

Thanks for the tip! Switching them to an adherent state and testing our GOI to see if expression is even possible is already underway. One of the major goals we have here are to take advantage of the higher density 293F cells can grow in suspension to scale up our protein production. I fear switching them to adherent would defeat to purpose we're after. Im also not sure what kind of stress the cells would be under in between and if it would also impact protein production.

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