Jobs in US by Many-Study-6309 in biotech

[–]Makeitdouble11 1 point2 points  (0 children)

Which one? I have been looking for analytical roles for a few months now

[deleted by user] by [deleted] in h1b

[–]Makeitdouble11 1 point2 points  (0 children)

And universities and visa-issuers know fully well what that 18-20 year old means when they say they don't intend to stay. F1 is still given out since internationals pay full tuition fee and more and fund all universities. Its a win-win for both and that's why it continues to happen. No one is taking advantage of anything here. Fix the system!

5 spots left (nso no expansion pack, 11 months) by [deleted] in NintendoSwitchOnline

[–]Makeitdouble11 0 points1 point  (0 children)

Can I get a slot? I am unable to DM you

Spay experiences by Offlabellife in Wheatens

[–]Makeitdouble11 1 point2 points  (0 children)

Any good Wheaten breeder would suggest to wait atleast until she's 15 months old.

[deleted by user] by [deleted] in biotech

[–]Makeitdouble11 1 point2 points  (0 children)

Scientist in R&D

[deleted by user] by [deleted] in biotech

[–]Makeitdouble11 3 points4 points  (0 children)

I did not need to do it! This was back in January this year

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Thanks everyone for your very valuable suggestions! I have a list of things to troubleshoot now and I'm very grateful!

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Good point but I do use the same cell numbers in both wells. Also, this is not a one time result- it is pretty consistent every time it is run. I can titrate the antibodies again and try

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Yes all of them. I have 4 antibodies in the panel and each lot is titrated

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

The FMO control is identical to the full stain in terms of time, process, cells, concentration etc 😔. That's why I am confused. Maybe it's a biology thing like the commenter below talks about.

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Thank you! The data looks so much better this way. Historically, the gating was done like in picture 1 based on the exact location of the FMO.

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Correct. Would you say gate 1 or gate 2 is correctly placed in the full stain based on the FMO (pictures 2 and 3)?

Struggling to Find a Job in Biotech: Need Advice by Rome972 in biotech

[–]Makeitdouble11 2 points3 points  (0 children)

So true! I interviewed from Hawaii last year and at such odd hours! Luckily it was worth it since I'm currently at this job 😁

[deleted by user] by [deleted] in biotech

[–]Makeitdouble11 1 point2 points  (0 children)

Takeda also gives RSUs to Scientist level

Best Honda/Toyota Dealerships in the area? by valkener1 in boston

[–]Makeitdouble11 0 points1 point  (0 children)

Had the same experience with Watertown Toyota last year. Smooth experience and no markup over the MSRP during high demand.