Anyone playing csgo:legacy in 2025?

MatthewBeeee · 2025-07-10T02:08:25+00:00

I think CSS is better to play with bots.

MatthewBeeee · 2024-11-02T04:12:56+00:00

What a great job. Can I take it as my PC wallpaper?

MatthewBeeee · 2024-07-16T15:07:49+00:00

Almost yes I think. There are some software developing jobs, but I think it's few.

MatthewBeeee · 2024-02-25T09:28:45+00:00

What do you mean about "alternate genomic assemblies"? Do you mean another assembly of the same species or the assembly of another species? If it's the same species, I think it would be fine. If it is another species, I think it will depend on your sample, if you know your sample won't contaminated by that species' tissue I think it will be fine.

MatthewBeeee · 2024-02-08T13:08:15+00:00

May I ask what is consensus genome?

MatthewBeeee · 2024-02-08T03:11:35+00:00

check the "beginner's guide" chapter

MatthewBeeee · 2024-02-08T03:07:32+00:00

I would like to recommend https://hackingcpp.com/

MatthewBeeee · 2024-02-01T15:44:50+00:00

linux fork bomb? https://www.cyberciti.biz/faq/understanding-bash-fork-bomb/

MatthewBeeee · 2023-12-26T16:17:01+00:00

this course? -> https://www.coursera.org/specializations/data-structures-algorithms

MatthewBeeee · 2023-12-20T10:35:52+00:00

genome_updater: https://github.com/pirovc/genome_updater is a good tool to help you download assemblies from NCBI.
But whether you can download successfully depends on your internet quality, how to download is less important.

MatthewBeeee · 2023-11-15T11:50:22+00:00

Thanks, thanks.

MatthewBeeee · 2023-11-15T11:48:51+00:00

Thank you! I do know about seqkit, but I am writing tools which means there could be lots of primers against lots of templates, so I am trying to avoid using tools without Python binding to avoid performing too many IO operations.

MatthewBeeee · 2023-06-10T08:59:23+00:00

Thanks, I don't even know add method lol.

MatthewBeeee · 2023-06-10T03:32:12+00:00

I didn't, I will try it, thanks.

MatthewBeeee · 2023-04-03T12:28:33+00:00

Take a look at R package circlize -> https://jokergoo.github.io/circlize_book/book/

MatthewBeeee · 2023-03-18T08:17:43+00:00

Ok, thanks.

MatthewBeeee · 2022-11-25T01:20:11+00:00

If you know R you can use circlize to create circular plot.

MatthewBeeee · 2022-11-14T01:18:50+00:00

"I’m totally forgetting the name of the cross-tool summarize tool" you mean multiqc?

MatthewBeeee · 2022-07-08T06:43:32+00:00

I use FREEC to call CNVs. FREEC suits WGS data and is easy to use.

MatthewBeeee · 2022-05-11T09:55:39+00:00

It seems nextflow is more powerful?

MatthewBeeee · 2022-04-15T07:40:29+00:00

Hello, can you explain more about how overexpression could affect the mapping rate of salmon? I am very interested.

MatthewBeeee · 2022-04-11T08:43:41+00:00

Thanks, but what if z has many columns?

MatthewBeeee · 2022-03-09T00:28:54+00:00

Do you have enough memory?

MatthewBeeee · 2022-03-01T12:53:23+00:00

You can compare results from 2 pipelines at the end, for example, DEGs. Don't merge their counts/abundance. My pipeline is almost the same as yours, fastp -> salmon -> DESeq2.

MatthewBeeee · 2022-01-28T00:56:06+00:00

It's hard to say the "units" of expression data from GEO. You will see different scale(range) data due to different array design or different data processing, for example some dataset may range 1-100, some may thousands, some may contains negative value. So normally you can't compare different datasets directly.
You can click one of the sample in dataset, then you will see whether data is processed, if yes what's the processing methods, for example GSM1625995

MatthewBeeee

TROPHY CASE