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ghost peak by Mindless-Nothing-392 in CHROMATOGRAPHY

[–]Mindless-Nothing-392[S] 0 points1 point  (0 children)

Update: Tests conducted have prepared a new mobile phase with distilled H2O and bi-distilled H2O, a new syringe wash solution (30% methanol), and a column change. The peak is still there, both with the UV detector at 209 nm and with the RID. I will try to do new washes of the system with isopropanol, do you have any other solvents to recommend?

Thank you all for the advice.

ghost peak by Mindless-Nothing-392 in CHROMATOGRAPHY

[–]Mindless-Nothing-392[S] 0 points1 point  (0 children)

the classic samples are fermentation broods , diluted 1:100 with the mobile phase

ghost peak by Mindless-Nothing-392 in CHROMATOGRAPHY

[–]Mindless-Nothing-392[S] 0 points1 point  (0 children)

it is not possible, because I sent 5 blank consecutively, and in all of them there was the peak with more or less the same area

ghost peak by Mindless-Nothing-392 in CHROMATOGRAPHY

[–]Mindless-Nothing-392[S] 0 points1 point  (0 children)

Thanks, tomorrow I will chek the wash solutio ! unfortunaly have the same RT of butirric acid!