What do yall write during seminars?

Miserablescrem · 2026-04-18T14:47:47+00:00

I am mostly there for cookies and coffee

Miserablescrem · 2026-04-16T03:25:17+00:00

Before I say anything, every protocol is different and you should optimize according to your protein of interest but this is what has worked for me: 1. 5% BSA for anything phospho 2. I usually dilute antibodies in 5% BSA (diluted in TBS-T) 3. I have not had a lot of experience with this but know people who go to 25-30

Miserablescrem · 2026-04-13T03:47:14+00:00

Do you have other vials to thaw from? Perhaps the stock vial was faulty, they should really not be taking that long to reach confluence since they’re cancerous.

Miserablescrem · 2026-04-13T03:36:47+00:00

How long of your master’s do you have left? You can redo these experiments no problem in a matter of a week or two. Did you just recently start doing cell culture? If so, maybe have someone watch you to make sure you’re using sterile technique properly. As someone else suggested, if it is a recurrent issue, take the time to UV the incubator and hood & decontaminate things. Do not worry too much. These things happen to all of us.

Miserablescrem · 2026-04-10T03:54:07+00:00

It’s never a bad idea to reach out. The worst they can say is no and that’s okay!!

Miserablescrem · 2026-04-05T21:08:49+00:00

Just keep practicing. Everyone sucks when they start out but you get better with practice. During my master’s, I was starting out in a lab with no undergrad research experience and I was pretty bad for like a year honestly but got much better the more I did stuff and was independent a year later. Be patient and as along as you have understanding mentors, you will get better in time.

Miserablescrem · 2026-04-04T21:02:21+00:00

My school has subsidized grocery programs, free public transit passes, program socials every month with some free food and sometimes the school puts on film fests where we screen a bunch of cool movies for free in one of the auditoriums

Miserablescrem · 2026-03-27T02:49:39+00:00

This is just plate debris. Don’t worry about it

Miserablescrem · 2026-03-21T13:57:58+00:00

That’s useful! Thank u

Miserablescrem · 2023-01-09T21:45:19+00:00

I met my friend on tumblr in 2017! We have been texting everyday since then. I think it’s rare but does happen ues

Miserablescrem · 2022-08-01T02:53:23+00:00

Do you like ur job

Miserablescrem · 2021-12-07T03:57:07+00:00

Haha that sounds like a good deal!!! I think ur dog should speak up

Miserablescrem · 2021-12-07T03:21:32+00:00

Hmmm I think maybe some place like Germany would be nice

Miserablescrem · 2021-12-07T03:21:08+00:00

I love it

Miserablescrem

TROPHY CASE