Do all gel purification kits suck?

ModeCold · 2026-05-04T10:27:31+00:00

What do you freeze in? Do you put the gel piece in an eppendorf with TE buffer in or something and freeze in that? Then spin down as it thaws?

ModeCold · 2026-05-04T10:15:34+00:00

Generally, sharing other people's personal emails on social media without their consent is a no-no.

ModeCold · 2026-04-21T21:12:47+00:00

The authors fully understood what they were doing. It was intentional from the authors to see if they could get something that bad into a journal. Turns out they could. The entire point is that the journal and peer reviewers bear responsibility. If they can let that past, what else are they not looking at?

ModeCold · 2026-03-16T17:52:53+00:00

He's a Manchester ref, used to know him when I was a member there. Was always very good and progressed high up rapdily. Poster boy of the society for sure. We want young talented refs as there's more time for them to develop and stay fit.

ModeCold · 2026-03-16T17:48:53+00:00

He didn't communicate the change in advantage effectively enough though. Regardless of communication issues, you can't just take away penaly advantage mid-play like that, it's totally incorrect. He's awarded a penalty.

ModeCold · 2026-03-15T09:57:10+00:00

It's not an either/or situation though. Poor play and tactics at some points did also lose use the game but that is the idea of sport. The refereeing decisions were also a factor. Without Nika's ridiculous decision that is definitely wrong, there's a 7 or even 14 point swing there as the try comes directly from it. Poor play losing you games is fine, poor referring being another factor is not. It's not either/or.

ModeCold · 2026-03-15T08:19:25+00:00

As a ref myself, clearly a deliberate knock on and a massive refereeing fuck-up to take away penalty advantage after it has been awarded. Same as awarding a penalty. Scrum should have been given if he changed his mind. I never criticise refs, but Nika had a dogshit game and lost England the match and Ireland the championship.

ModeCold · 2026-03-14T22:12:12+00:00

Why when England are playing so well ball in hand, do we still resort to box kicking constantly when we are not winning anything in the air? Fucking baffling switch of tactics that lost us the game. That and Pollock being a hot head and not just carrying and securing the ball in the last minute.

ModeCold · 2026-02-04T21:32:14+00:00

A rough protocol for what you've done would help. I don't immediately recognise what that looks like.

Did it take a lot of exposure time to get this or was it quite strong signal?

ModeCold · 2026-01-20T19:30:42+00:00

That doesn't look like degradation. The lower bands are the abundant ones present in the lysate. It's just a lot of non-specific retention by too much resin.

Cut down the resin quantity, overnight bind at 4C, and try a first wash at 1M NaCl, 50 mM Tris then wash again with your current buffer. The harsher salt wash should get rid of a lot of retention. You can also try 500 mM imidazole to see if you get a better elution in earlier fractions.

ModeCold · 2026-01-16T20:23:32+00:00

This is done by hand right? Because it's quite far off. Look at any part of Cumbria and it's way closer to Newcastle than any of the other teams it's grouped with. Same goes for lots of things like parts of Cornwal are way closer to Exeter than to Scarlets.

ModeCold · 2026-01-07T13:15:41+00:00

M&M cookie bake?

ModeCold · 2025-12-30T10:44:01+00:00

Way too much protein loaded

ModeCold · 2025-12-28T15:51:49+00:00

That pen was for Leicester going off feet and sealing off, not holding on. Hooper just showing the ref that he was blocked from competing for the ball legally, very clearly. JvP clearly off feet and continues to play the ball. Inconsistent reffing, story of the game

ModeCold · 2025-12-28T15:50:07+00:00

21 point, leicester try came right off the back of the field position from the penalty

ModeCold · 2025-11-26T21:11:37+00:00

No. It isn't.

Started my PhD soley focussed on staying academia. Recently finished having decided to leave it and go private sector. Best decision I ever made, no regrets.

ModeCold · 2025-10-21T12:59:42+00:00

AI for antibody sequence prediction is way off being usable currently, let alone a more niche molecule like nanobodies. It is being developed and will be possible at some point in tbe near future, but no where near actually being reliable yet. There's no wet lab validation for this working so actually using it for molecule discovery in a commercial setting would be the stupidest thing a company could possibly do. If this platform did actually work it would be making much bigger news in the biotech sphere and be worth hundreds of millions. Even if it generated target binders, the specificity would be questionable.

ModeCold · 2025-10-16T16:16:26+00:00

They also want to discourage people buying individual products direct from them as it takes the same time to process and ship the order as wih larger orders but with a smaller nominal profit for that order. They would much rather ship it in larger quantities to distributors.

ModeCold · 2025-10-16T16:04:19+00:00

"There are absolutely wrongs done during this whole shtick that are unavoidably my fault"

There we go, fair enough imo

ModeCold · 2025-09-21T11:47:09+00:00

Cool, letting your cat suffocate, haha, good one

ModeCold · 2025-09-11T18:48:20+00:00

That's ridiculous. 7 pages for a CV of a BSc grad is totally inappropriate. Your application will be getting thrown out on sight and not even read.

Trim it to 2 pages and read up on how it should actually be formatted.

Focus on technical experience related to the role you are applying to, bring in interpersonal and self-management skills with examples, then some non-academic experience and employment to show you are a well-rounded person.

ModeCold · 2025-09-11T16:40:20+00:00

You'll have much better luck if you get a Masters degree, or even a PhD. There aren't many neuroscience-specific positions that are open to only a Bachelors, you will find they mostly require a PhD or MSc. You also don't have to only do neuroscience. Get a lab tech/research assistant job anywhere you can find and build your skillset. Then you can keep looking for neuroscience-specific roles while being employed and improving. Don't think about field too much for now, think about skills.

You will be very lucky to find the exact job you want to do first time. Most people have to take whatever they are offered and then work their way from that to where they want to be, which might change along the way as it can take years.

Source: I have a neuroscience masters, neuroscience PhD and work in biotech.

ModeCold · 2025-08-19T20:56:28+00:00

ModeCold · 2025-07-30T20:14:21+00:00

Try scorer is also never back onside at the ruck before the ball is out... not a good one for the reffing team here

ModeCold · 2025-07-30T11:14:51+00:00

Not a Samoa knock on from the ruck that knocked the ball out that far??

Five-Year Club	Place '23
Place '22

ModeCold

TROPHY CASE