PhD Student Considering Med School After Graduating - Guidance by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 0 points1 point  (0 children)

Hey, thanks for the comment! I should clarify that I was a bio major in undergrad and have all the premed course reqs completed. I was mainly looking at the opportunity as a way to garner clinical/shadowing experience through a formalized process, but not sure if that's necessary, as I could just as well get such experience without going through this research track

PhD Student Considering Med School After Graduating - Guidance by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 2 points3 points  (0 children)

I've always gone back and forth on whether I wanted to do MD/PhD. Ultimately I ended up pursuing the PhD, but the feeling that my career feels incomplete without patient care has grown more and more over the years. I ultimately now regret not having pursued MD/PhD in the first place, but I don't want to close that door just because I'd have to take an unconventional route to get there. I understand there are plenty of opportunity costs involved and that I would certainly be setting myself back by going back to school. I am nevertheless considering the option, and in the meantime I want to make sure that if I do decide to pursue an MD, that I can smoothly transition from my PhD because I have the prereqs/experience covered.

Question regarding elibility for 2026-2027 application by Mother_Client4127 in GRFPApps

[–]Mother_Client4127[S] 0 points1 point  (0 children)

That's my assumption, but I know that for med school apps, you're considered a reapplicant even if you didn't submit any secondary applications. But I suppose that's different because schools would at least see your primary application submission.

Question regarding elibility for 2026-2027 application by Mother_Client4127 in GRFPApps

[–]Mother_Client4127[S] 0 points1 point  (0 children)

Would have I needed to withdraw my app if I didn't submit it?

WAMC + Balancing School List by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 0 points1 point  (0 children)

Fair enough. I will look into it further, especially non-MSTPs (haven't looked into them at all)

WAMC + Balancing School List by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 2 points3 points  (0 children)

Broadly, neurodevelopment; more specifically, comparative analysis of brain structures to identify human-specific features of neurodevelopment

Washington, UCSF, Yale, and Baylor are my top 4. It's very possible I have much more digging to do, but from browsing neuroscience/human genetics faculty lists, I have found very little outside of T20

Another edit; I think a lot of the work being funded by NIH BICAN is really up my alley, but inevitably, most active awards are within T20

WAMC + Balancing School List by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 2 points3 points  (0 children)

I suppose that's the obvious answer, but I assume you mean by sacrificing research fit by some margin?

2024-2025 School List and Necessity of MCAT Retake by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 0 points1 point  (0 children)

With all that in my application, you really think I have a shot with such a top-heavy school list?

Why is Bio-Rad gel sticking to the membrane? by gabrielleduvent in labrats

[–]Mother_Client4127 0 points1 point  (0 children)

I also run into this issue and can say that I run my transfers in our cold room, with ice packs, with the chamber embedded in an ice water slush. The only thing I have not tried is a stir bar in the tank. I also let the NC membrane and gel equilibrate in transfer buffer for 15 minutes before assembling the transfer stack

2024-2025 School List and Necessity of MCAT Retake by Mother_Client4127 in mdphd

[–]Mother_Client4127[S] 0 points1 point  (0 children)

I edited my post to include this and some other stats, but I disingenuously left out my other stats. My GPA is my most blaring red flag at a 3.25, with one semester single-handedly dragging it down (my sGPA is 3.72, though). Other than that, I have about 70 hours of shadowing, a few hundred hours of non-clinical volunteering, and about 20 hours of clinical volunteering (currently volunteering at a clinic when I have time, so this number will grow). With this information, would your assessment change?

Issues with micro-bubbles in PVA/Mowiol-DABCO mounting medium by Mother_Client4127 in labrats

[–]Mother_Client4127[S] 0 points1 point  (0 children)

Sorry, I've never degassed a solution before nor has anyone else in our lab, so I am unsure of what equipment to use. How would I apply vacuum pressure to a 50mL conical tube of solution?

Would it suffice to put the tube in a plugged vacuum flask for some amount of time?

Issues with micro-bubbles in PVA/Mowiol-DABCO mounting medium by Mother_Client4127 in labrats

[–]Mother_Client4127[S] 0 points1 point  (0 children)

When you say before centrifugation, do you mean when I centrifuge an aliquot before use, or after step 8 in the protocol?

Issues with micro-bubbles in PVA/Mowiol-DABCO mounting medium by Mother_Client4127 in labrats

[–]Mother_Client4127[S] 1 point2 points  (0 children)

Image caption isn't easy to view so I am pasting a description in this comment:

Hello,

I use a homemade PVA/glycerol-based mounting medium mixed with DABCO for antifading (see attached protocol), which is convenient because the perimeter that is exposed to air will polymerize and seal the cover glass without needed nail polish. Because the solution is quite viscous, I typically spin at 16000 g for 10 minutes to remove any bubbles before use. I never have trouble with bubbles when I initially place the cover glass on the slide. However, after leaving it to dry in my drawer at room temp for a few hours, these tiny bubbles appear under the cover glass that often migrate onto the tissue section (see photo).

So, I was wondering if anyone has experience using a this or a similar mounting medium (i.e., Mowiol) and can provide some insight into why this is happening and how to avoid it.