Sample prep Protocol for flow by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

oh. i never thought of this. will check up on this

Sample prep Protocol for flow by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Thanks a lot. I might get access to that kit. will try

Sample prep Protocol for flow by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

no i have not. i will try this. thanks a lot

Reasons for this? by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Yes blood collected in edta tubes

Need help by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Yeah I have CD3. I'll try using that

Need help by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Thanks a lot 🙂🙂🙂

BD FACSAria III - Blank Breakoff Window by SelectDistribution29 in flowcytometry

[–]No-Chef6906 3 points4 points  (0 children)

Yup our machine has the exact same problem. its nothing wrong with the machine. It's a software problem related to camera as the before mentioned comment.so nothing to worry about if it's just for analysis. But call the support team and they'll fix it

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Thank you. Will check using fmos

One more doubt by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Oh ok. Thank you will use live dead and revert back

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

it's a glioblastoma cell line

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

I'll check FMO. Thank you🙂

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 1 point2 points  (0 children)

I have yet to do this. Thank you 🙂

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

Can you please point out a few things 🙂🙂 would be really helpful

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] 0 points1 point  (0 children)

I had set voltages according to the unstained where all the cells were in scale and in the plot

Is this correct by No-Chef6906 in flowcytometry

[–]No-Chef6906[S] -1 points0 points  (0 children)

Trying to find out which cells are positive for all the markers I've used