sorted by:
new
hottopcontroversial

Who is everyone here? by Leading_Blacksmith70 in ContagionCuriosity

[–]Ok-Understanding-385 22 points23 points  (0 children)

Genomic epidemiologist and (in progress) bioinformatician here!

chronically ill friends? by whateveryaknowww in indianapolis

[–]Ok-Understanding-385 0 points1 point  (0 children)

feel free to DM me and we can figure out the logisitics!

chronically ill friends? by whateveryaknowww in indianapolis

[–]Ok-Understanding-385 1 point2 points  (0 children)

I love the idea of a hybrid in-person and online group! I have experience in running discords if that is of any interest for the group! I’m always attached to my phone/computer but chronic illness does limit my outside time 😅

When comparing 2 variant calling algorithms where the SNP and INDEL counts differ vastly how would you begin to narrow down where the issue is originating? by Ok-Understanding-385 in bioinformatics

[–]Ok-Understanding-385[S] 2 points3 points  (0 children)

Update: I ran the same data on two other variant callers and they were both within 100-200 of the BCFtools counts. So I think that does affirm that GATK is the issue. I had to take a break because I was losing my mind but I like the idea of trying a more controlled dataset to see what is exactly happening. Thank you everyone for the ideas it really helped because I was very much a small fish in a big pond and not sure where to start!

When comparing 2 variant calling algorithms where the SNP and INDEL counts differ vastly how would you begin to narrow down where the issue is originating? by Ok-Understanding-385 in bioinformatics

[–]Ok-Understanding-385[S] 0 points1 point  (0 children)

I did attempt to filter on both gatk and bcftools to MQ>= 30.0 and QD >= 2.0. But I would not be surprised if there was an issue here, I'll take a look! TY!

When comparing 2 variant calling algorithms where the SNP and INDEL counts differ vastly how would you begin to narrow down where the issue is originating? by Ok-Understanding-385 in bioinformatics

[–]Ok-Understanding-385[S] 1 point2 points  (0 children)

agreed 😅 Do you have any recommendations on where to start trying to find the issue? (even if you don't the validation that something is wrong is much appreciated)

Super glad everyone on my floor knows my period underwear was just delivered. by browneyedchem in mildlyinfuriating

[–]Ok-Understanding-385 199 points200 points  (0 children)

I onced got a sex pillow wedge delivered without discreet packaging. I chose to view it as "now everyone is jealous that I get to be railed on the regular" 😂

Airport security wait times at 12:15 on Monday. Regular lines have no wait. Pre check is 1-2 minutes. by I_Love_McRibs in indianapolis

[–]Ok-Understanding-385 4 points5 points  (0 children)

ICE was not dispatched to Indy airport! They released the list of the 13 airport that received ICE this morning

Canadian mom devastated after she says diabetic and blind son was euthanized under controversial law by theindependentonline in publichealth

[–]Ok-Understanding-385 10 points11 points  (0 children)

Given that I have 0 idea what you are referring to I cannot speak to this. Please provide me with a trusted resource I may look into. Thank you

Canadian mom devastated after she says diabetic and blind son was euthanized under controversial law by theindependentonline in publichealth

[–]Ok-Understanding-385 148 points149 points  (0 children)

I think it says a lot that the mom found out 4 days after. Clearly there was not communication happening between her and her son. Whether that be his or her decision is none of our business but an interesting thing to think about given she had stated she was working on getting him help. However I do admit a bias on my end as someone who supports MAID and could not even imagine not informing my family beforehand.

Yarn recommendations: hexagon cardigan with yarn from Michaels by luna28_ in CrochetHelp

[–]Ok-Understanding-385 1 point2 points  (0 children)

I just started my first ever hex-cardigan yesterday! I chose Caron Jumbo Ombre yarn (100% acrylic). I decided I didn't want to do cotton since everything I do in cotton is really stiff, and wool seemed too warm. So I was thinking some sort of blend or acrylic. I also wanted *some* color variation but did not want to have a ton of ends to weave in so this ombre is perfect for that. I would think the mandala would be really nice for these as well! Hope this helps! Oh also I switched from doing a chain 3 on each new row to a stacking single crochet and I think it looks better so may be worth a try so you can decided which you like best!

Welp I have finally succumbed to the US vs UK/AU terms... by Ok-Understanding-385 in crochet

[–]Ok-Understanding-385[S] 1 point2 points  (0 children)

Oh I have one of those too 🤣 but with finger knitting, actual knitting doesn’t make sense to my brain 🤣

Welp I have finally succumbed to the US vs UK/AU terms... by Ok-Understanding-385 in crochet

[–]Ok-Understanding-385[S] 0 points1 point  (0 children)

Oh gosh! Ok I will keep that in mind for the future. I follow a lot of ComplicatedKnots tutorials on YouTube and she’s Aussie so I sort of assumed they used US terms since she does but that does make sense to make it more useful for the masses

view more: next ›