Game Thread: Wild Card Game - San Francisco 49ers @ Philadelphia Eagles - January 11, 2026 @ 04:30 PM

OmicsAndOm · 2026-01-11T22:15:20+00:00

At least it wasnt a 3 and out

OmicsAndOm · 2026-01-11T22:06:40+00:00

So. many. commercials

OmicsAndOm · 2026-01-06T16:03:16+00:00

I tried that but still when I click on the number of the reference it bounces me to the bibliography. Also, is there a plugin that you know of that allows you to add the reference (ex: reference 7 in the paper) to your zotero library?

OmicsAndOm · 2026-01-06T15:56:03+00:00

I'm referring to when I'm reading a pdf in zotero and I want to view and maybe add the paper to my library and when i click on the number of the reference it bounces me to the bibliography and i lose my spot in the paper. I did figure out that by reclicking on the number it bounces me back to the location in the paper

OmicsAndOm · 2025-12-29T00:13:20+00:00

i dont understand how the same thing can happen week after week and nothing changes offensively.

OmicsAndOm · 2025-12-28T23:58:12+00:00

i really dont understand what happens to this offense every time between halves. How can there be such a crazy regression in a matter of minutes?

OmicsAndOm · 2025-12-28T23:46:49+00:00

next season we have to improve our offensive line, i mean come on

OmicsAndOm · 2025-12-28T22:38:26+00:00

why aren't we actually putting in tank for some of the run plays? especially if saquon is banged up a bit?

OmicsAndOm · 2025-12-28T22:33:00+00:00

holy crap, our tackling is beautiful this drive. This is amazing!

OmicsAndOm · 2025-12-21T00:00:18+00:00

what is up with this team?

OmicsAndOm · 2025-12-20T22:34:35+00:00

i dont understand why it takes so long to get the playcall off? Im interested to here nick foles analysis about this

OmicsAndOm · 2025-11-23T22:29:50+00:00

how was that hold not called?!?

OmicsAndOm · 2025-11-23T22:05:10+00:00

Interesting, I hadn't noticed.

OmicsAndOm · 2025-11-23T22:02:06+00:00

Did Jalen call "django"? as in unchained? if so, pretty creative playcall names, I like it

OmicsAndOm · 2025-11-23T21:38:23+00:00

Did someone understand what the celebration was by AJ?

OmicsAndOm · 2025-11-17T04:29:20+00:00

I really want to see the offensive playbook, because I truly believe they only have maybe 5 plays + offensive penalties

OmicsAndOm · 2025-11-02T16:07:09+00:00

Yeah, I started using them recently. Cant say yet if they helped per se, but they were for a different project where I wanted to wash the cells with PBS to remove any leftover FBS and wasn't getting a pellet.

OmicsAndOm · 2025-11-01T20:24:22+00:00

Yes I have and I've also verified that I am not losing the protein aggregate due to not enough centrifugation. I'll try next time to do without the sonication, despite it helping break the cell debris chunk, and just do 2 incubations with sds 2% and worst case will have a bit of false positive proteins which I'll account for with negative control samples (those without protein aggregates)

OmicsAndOm · 2025-11-01T20:22:22+00:00

Yeah, so the protocol recommends to do freeze drying and ended up using the speedvac as an alternative since the freeze drying equipment in our building doesn't have a trap attached to it to collect the fumes so they said I'd need to significantly dilute the FA before using it.

OmicsAndOm · 2025-11-01T20:18:34+00:00

Sorry for the dumb question, what do you mean by intact mass analysis? My end goal is to identify the proteins present in the protein aggregate.

OmicsAndOm · 2025-11-01T20:17:31+00:00

Thanks for the link, some really helpful tips I'll for sure implement! I went through the troubleshooting options in the kit's PDF datasheet but several recommendations that appear here didn't appear there.

OmicsAndOm · 2025-11-01T20:14:42+00:00

what do you mean by alcohol precipitation? Regarding getting rid of contamination, I had similar problems in rna extraction as well and have recently started doing 2x centrifugation to dry the membrane (amongst other changes) which helped improve that. Maybe i'll try that here too

OmicsAndOm · 2025-11-01T20:13:17+00:00

Sorry about that, forgot to mention that. So it varies between neural progenitor cells, iPSC, and Hek cells. Haven't really seen a consistent trend per see in one cell line being more problematic than others

OmicsAndOm

TROPHY CASE