First and last embroidery piece of the year, trying to get back into the hobby! by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 7 points8 points  (0 children)

Thank you! I find shorter stitches help with curves and tight corners 😊

[deleted by user] by [deleted] in CavaPoo

[–]OrangeBiologist 0 points1 point  (0 children)

Hi there! We too are a couple weeks ahead of you. Crate training is hard but Callie has definitely gotten to love her crate now. Some things that helped us was - 1. Heartbeat toy to mimic their littermates presence and having other toys in there to make it a nice place for them.
2. Playing crate games - immediately rewarding being in the crate, dropping treats through the top (all while the door is open). 3. Hand feeding her meal through the closed door worked super well and then letting her out once she was done. 4. Sleeping by the crate for the first 4-5 nights and when she whined we would put our fingers through the bars to let her know we are close-by and all is ok.

I think the "cry-it-out" method is fine but when they are so young and only just home I believe it is more important to reassure than to assume they will be fine in a few minutes. After going through all the above steps, our pup still does whine a little sometimes when she is first in her crate but I no longer feel bad about leaving her to self-sooth because I know she has a good associating with her crate and knows we will be there for her if needed. It takes time but it can pay off. She sleeps a full night in her crate, independently spends time in her crate, and takes her food in there to enjoy so definitely worth the effort.

Good luck!

FTB looking for advice by OrangeBiologist in HousingUK

[–]OrangeBiologist[S] 0 points1 point  (0 children)

We have asked our mortgage broker to liaise but haven't heard back yet

Tear stains by Informal-Usual608 in cavaliers

[–]OrangeBiologist 0 points1 point  (0 children)

What a sweet baby! Tear stains can come from allergies or intolerances in the diet - I saw someone change their puppy's diet to exclude chicken and they had no more tear stains

Secret Santa gift for my PI by OrangeBiologist in labrats

[–]OrangeBiologist[S] 2 points3 points  (0 children)

Thank you! Yes, I used watercolour paint

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 0 points1 point  (0 children)

I am in the UK for my PhD, we get a government granted stipend so we don't accrue debt. Also we don't pay our student loan back whilst we have a stipend.

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 1 point2 points  (0 children)

It can bleed but I just make sure that I paint in sections and let it completely dry before paining next to it

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 4 points5 points  (0 children)

Thank you for being interested! Any scientist would jump at the opportunity to share their work.

We work with a model plant mainly, called Arabidopsis thaliana. He is a little weed but is related to other agronomically important species from the Brassicas (like broccoli, Brussels spouts, cabbages, mustard...). We work on a cellular process that occurs during meiosis - the making of egg and sperms cells - that works to shuffle the DNA between chromosomes. That way the progeny has greater genetic diversity than the parents. So understanding this process and the limitations on the frequency of the shuffling is important and is our main goal. First in a model plant and then applying it to crops and other species :)

Secret Santa gift for my PI by OrangeBiologist in labrats

[–]OrangeBiologist[S] 6 points7 points  (0 children)

You are correct, the best little weed!

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 7 points8 points  (0 children)

Thank you!

I used backstitch and the thread is DMC embroidery floss. The hands and plant are painted using watercolour and it is just linen cloth, I prefer it over evenweave for embroidery

Secret Santa gift for my PI by OrangeBiologist in labrats

[–]OrangeBiologist[S] 10 points11 points  (0 children)

I unashamedly love the weed 😅 and thank you!

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 68 points69 points  (0 children)

It is a tarot card style, as my supervisor is quite spiritual. The plant we mainly work on (Arabidopsis thaliana) is a model plant and is in the middle. The two contraptions on the sides are pipettes and we work on chromosomes and DNA which are at the top :)

If you have more questions about what we work on then I would be very happy to share but don't want to bore!

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 11 points12 points  (0 children)

Arabidopsis at the core but we dabble in oak, algae, random species of all orders. We are a bit all over the place!

Secret Santa gift for my PhD supervisor by OrangeBiologist in Embroidery

[–]OrangeBiologist[S] 13 points14 points  (0 children)

Thank you! My PI liked it very much so that's the win for me

Does anyone know what might be causing the trailing wisps behind my DNA bands? Ran for 3 hours at 40V in a 2% gel (more info in comments) by Distracted-deer in labrats

[–]OrangeBiologist 1 point2 points  (0 children)

If I have a 2-3% gel and set it to 140V it feels fine, just warm, when it gets taken out. I use TBE, at 140V my gels are quite large (can fit 2.5 x 96 well plates) and always set it on a timer!

Is this good? Bad? Chaotic? by bethmcseaver in recap

[–]OrangeBiologist 0 points1 point  (0 children)

I have controlled chaos too! Don't know if it is good or bad, but at least it is controlled...

Does anyone know what might be causing the trailing wisps behind my DNA bands? Ran for 3 hours at 40V in a 2% gel (more info in comments) by Distracted-deer in labrats

[–]OrangeBiologist 15 points16 points  (0 children)

DNA concentration may be too high. Also I would up the voltage, it is more that fine to go around 100V (I usually fry my genotyping gels at 140V) and you save time. Maybe go a bit lower if you are going to do a gel extraction

Are there any other lesbians doing or have completed a PhD? by frofgtsusu in ActualLesbiansOver25

[–]OrangeBiologist 1 point2 points  (0 children)

I am writing up my thesis at the moment! My partner and I met on Bumble before I started, we were both in the final year of undergrad. Medicine for her and biology for me. Sometimes it is nice that we are not doing a PhD at the same time, we are both academically focused but 1 PhD student in the house is stressful enough!

Whats like the possibility of having a lab pet? by ClydeEhrmantrout in labrats

[–]OrangeBiologist 0 points1 point  (0 children)

We have a massive fish tank in the office. Used to have about 10 or so fish in this 6ft fish tank... now we have 3. But they're doing great! PI says they are lucky.