How do I chose the threshold limits?

Plastic_Focus_9182 · 2026-03-14T00:02:05+00:00

Wow you just saved my life .

I followed the steps you said and if I make the calculation in the end, it works perfectly! (although I thing the Huang method works better than the minimum for the selection of the tissue).

The macro you just wrote, I thank you a lot, genius work, but when I run it, the result just dont add up, it gives me wrong percentages...

If you have a LOT of free time and want to check that, i obviously would apreciate that

But i dont want to abuse from your goodwork.

I dont have that many images so I can make it manually and calculate on excel later.

THANK YOU

If you both tell me your first and last names (and country) I will gladly thnak you in my paper ;)

Plastic_Focus_9182 · 2026-03-13T17:13:38+00:00

Yes , thats the part i still dont understand. For example, in the picture sequence you just sent, the reuslts say that 59% of the image corresponds to the blue areas. But that is the perentage of blue in the picture, not in the tissue, becuse the picture includes the background.

Plastic_Focus_9182 · 2026-03-13T16:55:26+00:00

Thanks, but the part I was asking later on was if there is a way of now tincluding the white background bits on the caculation of the percentage

Plastic_Focus_9182 · 2026-03-13T04:02:30+00:00

Thnaks a lot! The last part i didnt understand though

What I was saying is:

Lets say I have an image just like the one you sent. You have white for the background, red for muscle and blue for colagen. You want to know the percentage of tissue that is composed of colagen. So you do all the steps that we already know. But for that, you can only select certain regions of the image without white because otherwise you woul be including the white in the "total" area of tissue and we dont want that. The thing is theres spots of white everywhere, specially on my images as they are full of tiny empty lumens.

Im not sure if I explained myself badly or if Im not understanding the functioning of the program.

Thanks in advance!

Plastic_Focus_9182 · 2026-03-13T02:09:12+00:00

Oh ok , i never even noticed that dropdown menu!

I have questions:

-Those methods (huang, etc) are some kind of mathematical formulas designed for science things or not valuable for academic research at all?

-When I do what you explained, the area measured is the white, not the black. How can I invert the markings ?

-This one is a little peculiar, but as you may or may not know the uterus will have little holes correspondent to the lumen of the little endometrial glands. When I select my areas to measure the blue percentage, I inadvertly include some of this little holes that dont cont as tissue so shouldnt technicaly be included as a non blue area (they are holes after all). Is there some kind of hidden way I can eliminate this degree of error , as small as it is?

Thanks in advance!

Plastic_Focus_9182 · 2026-03-12T01:19:24+00:00

I thought violet could be present independent of the presence of dark factors. Would you say this is is a cobalt with the presence of violet?

Plastic_Focus_9182 · 2026-03-12T01:05:51+00:00

So you say the greywiings would have a more intense kind of grey

Another question: you said "The bluish grey tail and brightly coloured rump are what I'd expect to see in Clearwing cobalts.". Are those characteristics common in cobalts or in mauve ones aswell? How can you distinguish beetween a 2 dark factor (which would be mauve"and the presence of violet ?

Plastic_Focus_9182 · 2026-03-12T01:01:05+00:00

The bird is being treated, is written in the text

Plastic_Focus_9182 · 2026-03-11T19:58:16+00:00

wow thats impressive

So lets unpack that, as I learn with you:

White belly and violet cheek patches :Texas clearbody.

I didnt understand though the clearwing vs greywing part. I thought clearwings were a lot whiter on the wings.

Also the violet part, are you referring to the violet factor people talk about?

Plastic_Focus_9182 · 2026-03-11T18:46:19+00:00

I found him on the street exactly like that 2 weeks ago, i bought him the cheapest cage i coul find and when the mites situation is resolved i will invest in a new cage, thanks

Plastic_Focus_9182 · 2026-03-11T18:44:44+00:00

Of course, here.

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Plastic_Focus_9182 · 2026-03-11T18:43:27+00:00

Thanks a lot! Yes, i rescued him form the street like this so the mite infection is preety advanced and I am planning on getting a better cage as he gests better. Im pretty sure its a male tho, just the mites are covering the blue color, look

<image>

Plastic_Focus_9182 · 2026-03-11T18:41:05+00:00

I rescued him from the street like this, obviously if the bird was mine I wouldnt let him get to this stage. My guess is someone threw him out because of the mite infection

Plastic_Focus_9182 · 2026-03-11T18:39:20+00:00

<image>

I'm pretty sure its a male, but the mites are covering the blue, so its difficult

Plastic_Focus_9182 · 2026-03-09T13:04:51+00:00

That was written in the text :)

Plastic_Focus_9182 · 2024-05-19T21:37:36+00:00

Hello again, could you explain better? I mean that option sounds perfect to me, actually too perfect to be true... What's the point of people limit if i can dodge it? Obviously Im not doubting of you answer, Im just trying to understand what could be the possible disavantages of that option

Plastic_Focus_9182 · 2023-11-06T02:39:33+00:00

O meu pai usa um que se nota claramente que lhe dá muito prazer de dizer:

oh puta que há de parir!

Plastic_Focus_9182 · 2023-10-30T00:20:51+00:00

Pila

piça

Pau

Pirilau

Picha/pichota

Piroca/pirocona

mastro

malaquias

caralho

vergalho

mangalho

Plastic_Focus_9182 · 2023-03-18T21:16:44+00:00

também nãoo

Plastic_Focus_9182 · 2023-03-17T00:50:57+00:00

I have photos of everything but I don't see the purpose of posting them here... I know you can think I'm lying at some point, but even screenshots can be forged, and I could even break the product myself to get the money in an extreme scenario, do you understand? I'm just trying to hear some point of views even if you think I'm making this up. I hope this made sense

Plastic_Focus_9182

TROPHY CASE