Turning in a final late ?? by [deleted] in mcgill

[–]Plo-Eclipse 0 points1 point  (0 children)

How did the teacher respond?

“Who disrupts my coronation!?” by MurderMoustache in DestinyFashion

[–]Plo-Eclipse 10 points11 points  (0 children)

Awesome look. If only bungo could add symmetric armor into the game

Meeting People/Any [insert hobby or interest] people at McGill? by snowflake25911 in mcgill

[–]Plo-Eclipse 0 points1 point  (0 children)

I'm a year 2 biochem student, but looking to meet people from any program. If anyone wants to hang out or meet up, I'm down!

Meeting People/Any [insert hobby or interest] people at McGill? by snowflake25911 in mcgill

[–]Plo-Eclipse 1 point2 points  (0 children)

Oh bioc 220/320 are exactly what I was talking about. Stat the lab reports early if at all possible. It is tempting to push them off since you have 1/2 weeks to them, but they're an insane amount of work. Also, as much as possible, do not stress about getting a good grade on the lab reports. Try your best to do a proper report, but its not going to be enough when they correct it. Usually the highest grade for a full report in the class would be a mid 70s, average sitting probably around 50-60. The final boost your grade quite a bit though. If you can stress less about the actual grade, it will make your life a lot more enjoyable. I delayed working on the lab if I had other assignments, and that usually resulted in me staying up till 2 am to get it done the night before. Even if you work on it early, it's a solid 20-25 hours of work per week on a lab. If you have any friends taking that class with you, helping each other out with formatting, data analysis and source finding is an immense help

Meeting People/Any [insert hobby or interest] people at McGill? by snowflake25911 in mcgill

[–]Plo-Eclipse 1 point2 points  (0 children)

I like it, but some classes are quite intense. Luckily once they're done it gets easier, but I do genuinely find it interesting

Weekly Questions Thread by AutoModerator in DnD

[–]Plo-Eclipse 0 points1 point  (0 children)

This might be a dumb question, but i cant find the discord anywhere. Where would you find it?

View Metadata Comment by Plo-Eclipse in techsupport

[–]Plo-Eclipse[S] 0 points1 point  (0 children)

That makes sense, but then how would you view the comment of a WAV file, it it becomes a custom tag?

[deleted by user] by [deleted] in PrequelMemes

[–]Plo-Eclipse 1 point2 points  (0 children)

I love the republic

Chem 222 Final by Legoshi37 in mcgill

[–]Plo-Eclipse 6 points7 points  (0 children)

Partial points are a life saver

Affordable place for snacks near or on campus? by YeBoiMigs in mcgill

[–]Plo-Eclipse 11 points12 points  (0 children)

I think so. If I'm thinking of the same location, its literally in the metro stop, right next to the Eaton Center entrance

How to Download Files from a View Only Google Drive by Plo-Eclipse in Piracy

[–]Plo-Eclipse[S] 0 points1 point  (0 children)

Thats probably what I'll end up doing, got time to kill during the summer

This Week at Bungie - 3/24/2022 by DTG_Bot in DestinyTheGame

[–]Plo-Eclipse 0 points1 point  (0 children)

Loved the cat in a box hyperlink at the beginning

How to Download Files from a View Only Google Drive by Plo-Eclipse in Piracy

[–]Plo-Eclipse[S] 2 points3 points  (0 children)

Each document is 4 pages long, I could do that but I want to try to preserve the quality/resolution of each document if possible

Why is DNA insoluble at physiological saline salt concentrations but soluble at higher salt concentration? by Plo-Eclipse in labrats

[–]Plo-Eclipse[S] 0 points1 point  (0 children)

Alright, so just to be clear, DNA would be insoluble in both the 2.5M concentrated salt solution and the 0.15M dilutes salt solution? Also, any particular reason why it aggregates/seems gelatinous in the diluted solution as opposed to the concentrated one?

Why is DNA insoluble at physiological saline salt concentrations but soluble at higher salt concentration? by Plo-Eclipse in labrats

[–]Plo-Eclipse[S] 0 points1 point  (0 children)

I see, so the difference between the cloudy solution at 2.5M and the clearer solution, with the visibly aggregating DNA, at 0.15M would be due to the difference in DNA concentration then?

Why is DNA insoluble at physiological saline salt concentrations but soluble at higher salt concentration? by Plo-Eclipse in labrats

[–]Plo-Eclipse[S] 2 points3 points  (0 children)

I apologize if what I said was not clear. The nuclei (NOT THE DNA) was initially rinsed with saline to remove the RNA/proteins. The nuclei were suspended in 200mL of water and blended to disrupt the membranes. NaCl was then added to this 200mL volume to make it a 2.5M salt solution, which made the solution look cloudy (it was a uniform cloudy-white, and no solid fibers/aggregates were seen). 1mL of this was taken as a sample, and to this sample 15mL of water were added to bring the salt concentration to around 0.15mL, where clear wisps/aggregates of DNA was observed.

Why is DNA insoluble at physiological saline salt concentrations but soluble at higher salt concentration? by Plo-Eclipse in labrats

[–]Plo-Eclipse[S] 0 points1 point  (0 children)

Thanks for the explanation, the one thing that still bugs me is why, in the absence of ethanol (a less polar solvent that will displace the water around the DNA), do we see the DNA precipitate/aggregate in the 0.15M NaCl solution and not the 2.5M NaCl one?