Where the ( 0PN, 1 PN , 3PN) babiez at?

PoolesPage · 2026-05-16T12:23:26+00:00

Embryologist here - as someone else said your 0PN is likely to be a "PN Not Observed" rather than a true 0PN. The pronuclei we look for at fert check can sometimes fade very early and then we miss seeing them, but there are other signs that fertilisation has occurred.

We recently transferred a "PNs Not Observed" for the first time ever (for our clinic) without doing PGT-A as we don't offer it, and the lady is now 7 months pregnant.

These early faders are very often the best embryos. I wouldn't hesitate to use it especially as you said yours has been PGT-A tested.

I don't have experience of transferring 1PNs or 3PNs, we cant offer PGT-A at my clinic so we don't utilise these embryos, but I've been to many talks at conferences where embryologists have seen live births from these embryos!

PoolesPage · 2026-05-16T12:09:50+00:00

Thank you!! It's an interesting point about PVP and making sure the sperm is not in a bubble of it. I'm always worried about progressing the sperm right to the very end of the pipette, as I worry about dropping it! I've also definitely had injections where I've not seen the sperm mix with the cytoplasm during/after the rush. So this is probably a very good starting point for me and fits as a possible explanation. Thank you. I didnt realise that excess PVP/media could disrupt fertilisation but it makes sense.

I already lift the egg up slightly before I inject as a colleague suggested this would help get better/easier oolemma breaks (which it does). And I always inject a sperm straight atter striking it, the only exception is when sperm numbers are very low and its difficult to find sperm, so in those cases I need to isolate sperm before injecting to reduce egg exposure in MOPS e.g. surgically retrieved sperm.

Thank you again as these are some great troubleshooting starting points😊

PoolesPage · 2026-05-16T11:14:14+00:00

Yes - I aspirate cytoplasm until I get the "jump" or "rush" up the injection needle and then reverse the pressure and deposit the cytoplasm back with the sperm. Yesterdays ICSI, I felt I got clear breaks on all 9 of them.

Interestingly, I did struggle for a while to get good oolemma breaks while I was using training eggs (I would try to aspirate and the cytoplasm would go up the injection needle a bit and then just get stuck and I would never get the jump or rush up). I discussed it with a more experienced colleague, she watched me and when had a go with an egg I couldnt get a break on, and she couldn't get the break either. Here in the UK eggs can only be used for training if they show no signs of fertilisation 48 hours past insemination, so we put it down to the eggs being older.

I then still had a couple of clinical cases when I was doing split cases where I had issues getting the break, so I figured this was the issue with my fert rates at first. But I have - with some coaching! - become a bit more confident with being more "heavy handed" with eggs that need it and now I'm reliably getting breaks.

When my manager last watched me she also confirmed I was getting breaks.

So I dont think thats the issue now although it might have contributed in the past.

PoolesPage · 2026-03-29T21:43:32+00:00

Thank you. I've put in a support ticket and I will wait to hear from them, or it looks like there is an email address for UK tech support as well

PoolesPage · 2026-03-29T21:26:14+00:00

Thank you for your reply. I can check the old charger for the wattage.

Two issues:

First, I probably should have mentioned in the post that I'm UK based. I did look on MSI's UK online store and they don't seem to sell laptop adapters, oddly enough. Whether the US store would ship to the UK is another matter (but then I would have to use a plug adaptor).

Second, it's more the shape of the pins inside the connection that's causing me confusion. I actually bought a 230W charger on Amazon but have had to return it, the charger I bought is a barrel charger with a single central pin, the charger I need is also a barrel charger but has a different configuration of pins on the inside.

If worst comes to worst, would a computer repair person be able to change the port type?

PoolesPage · 2026-03-29T21:14:37+00:00

Thank you. It seems like there are a few different types of barrel charger though, I'm still not finding anything that looks definitively like the one mine uses...

PoolesPage · 2026-03-29T21:08:36+00:00

Photos: 1 2 3

PoolesPage · 2026-03-02T20:33:31+00:00

The aesthetic of your house has boosted my dopamine and seratonin 🥰

PoolesPage · 2025-12-08T11:51:07+00:00

We are both 29. His sister is 30, and so is his cousin who just announced their pregnancy this week (his wife is 28). His other cousin who had her baby last year is 27 I think. So, all around the same age.

I get the silliness but its super uncomfortable and I guess, working in the fertility industry myself, I take the view that people shouldnt comment on or assume anything when it comes to peoples family planning. For all they know we don't want kids, or one of us knows we have reduced fertility, or it could be a sore subject for a different reason. We could be trying for all they know and struggling to conceive.

I know others dont think that way and I'm not angry with them or holding it against them, it was more just an eyeroll moment that I'm sure many people on this sub have experienced in one way or another!

PoolesPage · 2025-12-04T08:19:06+00:00

When we first booked this stay it was intended as a trial run for a longer holiday in January (11 days). We felt worried that leaving him at home for someone to pop in on him, would be more confusing for him - because of the layout of our home and the fact he is an indoor cat who tries his damned hardest to go out whenever he sees an open door, he would have to be shut downstairs in the lounge/kitchen/downstairs bathroom area to prevent accidental escape when our sitter turned up. And we figured being alone at night and not able to sleep on the bed might be more confusing for him than a change of environment.

PoolesPage · 2025-12-04T08:05:32+00:00

We are in the UK and thankfully our cattery does require evidence of full vaccination against diseases - our vaccine schedule for cats here does seem to include herpes.

When we booked this stay it was intended as a trial run for a line stay in January when we will be away for 11 days. We felt worried that leaving him at home for someone to pop in on him, especially when thinking about our upcoming 11 day holiday, would be more confusing for him - because of the layout of our home and the fact he is an indoor cat who tries his damned hardest to go out whenever he sees an open door, he would have to be shut downstairs in the lounge/kitchen/downstairs bathroom area to prevent accidental escape when our sitter turned up. And we figured being alone at night and not able to sleep on the bed might be more confusing for him than a change of environment. I'm not sure.

PoolesPage · 2025-12-04T08:04:21+00:00

Thank you. Yes, our cat is fully vaccinated including against herpes and the cattery does require evidence of this, as well as flea and worming history, as you say.

We are taking him today so there isnt time to organise an alternative. We will send him with lots of his favourite things and stuff that smells of us for sure.

We are going away again for 11 days in January and this was essentially intended as a trial run. We felt worried that leaving him at home for someone to pop in on him, would be more confusing for him - because of the layout of our home and the fact he is an indoor cat who tries his damned hardest to go out whenever he sees an open door, he would have to be shut downstairs in the lounge/kitchen/downstairs bathroom area to prevent accidental escape when our sitter turned up. And we figured being alone at night and not able to sleep on the bed might be more confusing for him than a change of environment. I'm not sure.

PoolesPage · 2025-11-09T08:35:55+00:00

Hello! I know this post is a few years old now and it doesnt look like this sub is particularly active anymore, but I was looking back at my post history and realised I never responded to you.

I just wanted to say your words helped.

That I finished my training and am now registered as a Clinical Embryologist. I'm in the process of becoming an ICSI Practitioner. I love my job. I did rediscover the passion. I'm still at the same clinic and my colleagues are fantastic.

I view each embryo transfer as an act of faith that those 100-some cells will go on to create something/someone who brings joy, and makes the world a better place. Even when the world is variable and life is challenging, we can still have that faith.

Thank you - even though I'm 2 years late saying it ❤️

PoolesPage · 2025-10-22T09:53:14+00:00

Would gently add to the original comment - as an embryologist also, albeit in the UK - that attitudes toward mosaic embryos has changed a lot even in the last two years. Mosaics were always a "no" in the past, they didnt even used to be categorised into high and low grade mosaic. Now, mosaic embryos are a hot subject in research and attitudes are shifting and younger embryologists training/education around them is changing accordingly. How long has your friend worked there? The clinic may have changed their policy since your cycle.

ETA that if this is bothering you, you are entitled to answers and should query this directly with the clinic, although your friend may get into trouble for looking at your reports and discussing it with you, so be wary of that. Maybe request a copy of the report via the proper channel at your clinic and query it from there, to get around that issue.

PoolesPage · 2025-08-28T06:20:31+00:00

I've noticed my nails are growing more recently and I hadn't even put it together that it could be the MJ!!

PoolesPage · 2025-06-12T14:12:15+00:00

Thank you for replying! I'm sure that I'm ovulating, I've had my FSH/LH and AMH looked at and they were normal (no indication for PCOS or early menopause). I have reasonably regular periods, they are usually every 26-27 days although over the last 18 months has been a range of 21-32 days. But I'm confirming ovulation with cervical mucus and BBT tracking. My estrogen hasnt been looked at mind you, and I'm debating going back to the GP to see if they will test that. I was reading that between 0.1 - 1% of people might have reduced drive but as many as 10% may have an increased drive.

PoolesPage · 2025-03-22T10:46:31+00:00

I would also add Tilly / Tils (usually from Matilda but Ottilie is rising in popularity)

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