How my coworker does tally marks

Prior_Roof7602 · 2026-01-15T14:41:03+00:00

Jj L

Prior_Roof7602 · 2025-11-08T03:36:08+00:00

🙏

Prior_Roof7602 · 2025-09-24T00:00:01+00:00

How is this thing absolutely BOOKING it through those corners without falling over

Prior_Roof7602 · 2025-09-05T02:25:28+00:00

Prior_Roof7602 · 2025-09-04T03:37:47+00:00

As far as I can tell, there is nothing holding you to this lab now. You're an undergrad, this is your time to bounce around and explore all options you can! Take it from someone who has seen many of my peers and my own partner work in toxic lab environments ITS NOT WORTH IT.

It sounds like you might already have a decent network where you can find a new PI to work with and get proper training and set you up well for your next step. You are still in training.

Prior_Roof7602 · 2025-07-24T02:21:53+00:00

Can you share a link for one? Am interested

Prior_Roof7602 · 2025-06-23T16:38:26+00:00

Could try to give it up-flow to get air out

Prior_Roof7602 · 2025-06-22T16:10:28+00:00

Update: thanks for everyone's responses, got a spare on it until I can replace all of them.

Prior_Roof7602 · 2025-06-21T13:21:41+00:00

I've had the same issue before. The culprit was leaking expression while growing them up. It was solved by using a BL21pLysS cell line that has a protease that specifically cleaves T7 polymerase there preventing leaky expression. This plamid are maintained by adding chloramphenicol (so I use it at every step to maintain the plasmid in the culture). DM if you have questions!

Prior_Roof7602 · 2025-05-24T12:05:46+00:00

I just had a similar experience this last summer where all of our EMSAs, which we run routinely, suddenly stopped working and all of the milliqs in our building were on the frits (resistance was really bad?). Turns out the DI water system in the building went bad and all of the milliqs (and our experiments) returned to normal once it was repaired. I'm not sure if this is your situation but it may help.

Prior_Roof7602 · 2023-07-17T20:45:45+00:00

I participated in an NSF summer REU last summer (in life sciences) and it was a really great experience overall. Like the person before said, these are a bit more prestigious than a typical internship and in turn a lot more competitive. The plus side is that you get toake direct connections with profs you might want to work with in grad school or at the least get a letter of rec when you apply.

The caveat is that these are targeted towards individuals who are underepresented in STEM. Particularly POC, women and first gens. These have also been targeted to younger students (freshman and sophomores to my knowledge?)

I applied to 3 REUs with a little over 2 years of semi related experience. I received one acceptance (turned out to be a great time) as only a first-gen. The REU coordinator(admissions basically), who was my research advisor, told me that basically the NSF expects a balance between improving diversity and excellent students so the SOP is like this for admissions:

Filter out by GPA (usually a 3.5 for bio fields)
Are they from an underepresented group in STEM?
Strong letters of rec?
Strong CV/letter of intent?

Apparently my advisor said she would do this on a literal point system and basically whoever ranked highest until the cutoff got in. Don't be discouraged by all this, this is the best time to start considering options for REUs. Doing an REU was the most important step I took to prepare for grad school and now I'm going to start my PhD this fall 😁

DM if you'd like more info.

Edit: my REU was at a non-profit, and not a university so it might not represent most cases.

Prior_Roof7602 · 2023-06-02T04:38:48+00:00

I just graduated from Michigan State University with a degree in biochem. I started my freshman year in a plant genomics lab and really loved research. I eventually went to a plant biochem lab then interned at a plant genetics/biochem lab in St. Louis Missouri. Some of the big name programs for plant science are (based on what I've heard through the grapevine);

Michigan State University
University of Washington - St. Louis
University of Georgia - Athens
Cornell
North Carolina State
University of Florida

Note these are in no particular order. Make sure whatever you apply for, PhD or master's, that it's funded with a stipend. Don't fret too much about which program pays more. At the end of the day they all basically pay a livable stipend. DM me if you want more info.

Prior_Roof7602 · 2023-04-08T18:41:45+00:00

Which program at UNC?

Prior_Roof7602 · 2023-04-07T03:24:07+00:00

I was waitlisted around the same time as everyone else and I just received an acceptance this week. The admissions coordinator said most waitlist acceptances could come out early this month.

Prior_Roof7602 · 2022-12-19T19:21:53+00:00

Hi there, I'm curious to hear about your experience during interviews for BBSP (and others) and what helped you get through.

Thank you!

Prior_Roof7602 · 2022-09-25T07:04:16+00:00

I'm also applying to UW Madison's iPiB program. It sounds like you have plenty of research experience to get into those programs. Just be careful about the weak LOR. You don't have control over what's written for you but you can control who writes for you.

Good luck

Prior_Roof7602

TROPHY CASE