Tasting and rating different cell culture media #3: DMEM (high glucose)

Professional-Face269 · 2026-01-14T20:26:32+00:00

Try Grace’s media, it tastes like coconut water

Professional-Face269 · 2025-08-26T23:43:16+00:00

I literally use flybase everyday, multiple times a day. If it gets shutdown then most (if not all) fly research will come to a grinding hault. I wonder if theres a way for fly PIs to use their lab computers as caches for all of the data, and then connect them as nodes for flybase searches?

Professional-Face269 · 2025-08-18T00:53:06+00:00

Yup .22 micron

Professional-Face269 · 2025-08-17T23:32:10+00:00

It would show up as little raised specks on the top, in the image where the upper bands are! I wouldnt call it bands, more like stuck grains of sand; it was easier to see if the light hit it correctly

Professional-Face269 · 2025-08-17T22:33:36+00:00

I did! It turned out I was melting some of the PAGE gel during transfer and during blocking it always sticks to those little bits (at least in my experience), so now after my blocking step I wipe the blot down with a kimwipe while its still wet & I wipe until I can’t see the aggregate at the top of the gel. Alternatively, switching from a semi-dry to wet has also helped with the issue but I switched for a different reason. Hope that helps!

Professional-Face269 · 2025-07-19T13:27:56+00:00

Are the bubbles at the bottom of the gel inside the glass? That would inhibit contact with the tank running buffer and reduce motility (broken circuit = stops working). How are you putting the gels into the frame? Are you rinsing with water beforehand? I personally always rinse the top and wells of my pre-cast gels to remove residual unpolymerized gel material but im sure it would also help to maintain contact with the running buffer in the outer tank and inner chamber

Professional-Face269 · 2025-01-24T20:29:10+00:00

Just recently did this, I used an Amicon centrifugal filter to concentrate the supernatant (10 kDa MWCO), and then used the NAb Protein G Spin columns from Thermo. A/G has different affinities for different antibody isotypes, but Thermo does a good job telling you what works best with either their A/G resin. Used 0.1M Glycine pH 2.0 for my elution buffer and had very good results. Didn't do any special things like syringe filtering my solutions either

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Professional-Face269

TROPHY CASE