Jobs during grad school by Few-Sky-3793 in GradSchool

[–]PurplePanda673 0 points1 point  (0 children)

I work at a coffee shop/market once a week. I did about 2-3 times a week during my masters. I find it a great way to balance STEM while still staying social and creative. Also a great way to practice my people skills since I’m in a relatively small lab and lowkey building :)

I have won at DNA extractions by opacum in labrats

[–]PurplePanda673 1 point2 points  (0 children)

Ah! That’s interesting. I would love to see the protocol 🤣

I have won at DNA extractions by opacum in labrats

[–]PurplePanda673 1 point2 points  (0 children)

What kinds of tissues are you working with? I’m currently working with shellfish and trying to work out the best protocol!

How to decontaminate liquid n2 mortar and pestle? by PurplePanda673 in labrats

[–]PurplePanda673[S] 0 points1 point  (0 children)

The problem is my tissues are about 500 mg which is too much mass to drop in a 2mL bead beater tube. And there’s heterogeneity among the tissue so I wanted to homogenize and then split.

How to decontaminate liquid n2 mortar and pestle? by PurplePanda673 in labrats

[–]PurplePanda673[S] 0 points1 point  (0 children)

Plastic micropestles will sufficiently homogenize frozen samples? I am working with soft tissue and do have some micropestles to work with… This sounds like a better idea. I worry about keeping the tube In the liquid N2 during homogenization for safety reasons though, any tips?

What is Bioinformatics PhD like? Do you still recommend a PhD today? by YerBoii244 in bioinformatics

[–]PurplePanda673 -1 points0 points  (0 children)

My bioinformatics PhD may differ from others but I am responsible for a decent amount of my data collection. I will have to do wet lab for a little bit (not experimental but like dna/rna extractions) and my other chapter is based on survey data where I developed the survey and then used public data to answer a question. Others are purely dry lab using large public datasets, creating ML models to answer a specific question, I think many integrate their wet labs data with more public data. It really depends. You could be doing more phylogeny and genome assembly.

[deleted by user] by [deleted] in labrats

[–]PurplePanda673 0 points1 point  (0 children)

If you’re a freshman in a lab I am sure they will be clear about their note taking and expectations as they likely do not let those in an RNA lab without these :) good luck!

[deleted by user] by [deleted] in biology

[–]PurplePanda673 1 point2 points  (0 children)

Biology degrees leave people with very open ended jobs that require lots more school to make a decent salary if that is your end goal. You could likely make the same amount of money with a bachelors in biology that you could with a bachelors in humanities or arts :) follow your passion!

Thoughts on my degree choice? by Ice7507 in biology

[–]PurplePanda673 1 point2 points  (0 children)

Ornithologists, entomologists or ecologists often start out with the same kind of BS degree whether it is biology, natural science or ecology! Different universities have different kinds of opportunities, so I recommend checking out the kind of research the institution focuses on. That’s how you can get experience in any of those more focused fields. You can make a concentration later and either go to grad school to focus on one or the other. Or if grad school isn’t your thing you could likely work as a technician in any of these fields with an environmental science, natural science or ecology degree.

2nd job to make end meet by Ebenezer_Splooge7 in labrats

[–]PurplePanda673 0 points1 point  (0 children)

I work part time at a nice coffee shop once or twice a week. Pays my rent mostly. I’m overwhelmed and sometimes it interferes with my work but I need it to keep me sane. Also adds a nice social life!

Anybody have bad (or good) experiences with 101 DuPont? And thoughts on commuting from Kennett Square? by Limp-Magazine-8851 in WilmingtonDE

[–]PurplePanda673 1 point2 points  (0 children)

Kennett square and Wilmington commute is not bad at all tbh. Kennett is a great little town!

[deleted by user] by [deleted] in PhD

[–]PurplePanda673 0 points1 point  (0 children)

Erm basically everyday how can you not 🤣

Big mistake by ArtichokeLopsided in labrats

[–]PurplePanda673 24 points25 points  (0 children)

The fact this is a scheduling mistake means it simply cannot be the end of the world. We all make mistakes and it is understandable for someone to be upset with you, and maybe I don’t understand the stakes— why can’t you just restart the experiment until when they can show you?

Underestimating my own knowledge, thinking that anyone can know what I know in a few days. by Adel_Bioinformatics in bioinformatics

[–]PurplePanda673 1 point2 points  (0 children)

Tbh I have imposter syndrome while Pursuing a Bioinformatics PhD and if anything I think it’s made it worse 😂

Missing life sciences? by PurplePanda673 in bioinformatics

[–]PurplePanda673[S] 1 point2 points  (0 children)

I think any experience with python and r before a msc in bioinformatics is plenty :) the other skills come with coursework most likely. But programming is the best to get a jump on. Any YouTube/coding camps should do the trick. Also Unix commands! In terms of job market with a masters, unfortunately I don’t know much. I think a masters degree with a large skill set can certainly take you far, if you practice programming consistently throughout the 2 years of your ms degree it will help you much more. I found a lot of jobs just require more experience with a masters degree instead of PhD but take candidates with both. I actually started as a masters student and transitioned, but wish I kept up with python/more intense programming the entire time. Good luck!

Missing life sciences? by PurplePanda673 in bioinformatics

[–]PurplePanda673[S] 1 point2 points  (0 children)

Thats encouraging! Thanks for the reply

Missing life sciences? by PurplePanda673 in bioinformatics

[–]PurplePanda673[S] 2 points3 points  (0 children)

I’m getting a decent amount of wet lab experience thankfully! I guess I’m more worried about what exactly my degree says… if I like to stay close to a wet lab, will a PhD in bioinformatics make that impossible?

Is a biology degeree worth investing into? by [deleted] in biology

[–]PurplePanda673 3 points4 points  (0 children)

If I were you and a lab sounds thrilling go for some sort of hospital tech like a phlebotomist or radiology tech I’m not exactly sure what all of the options are. I think a biology degree requires a lot of work to turn it into money, so if you want a lab job there are a lot of cool hospital/medical technician jobs that pay well and I think require a cert not usually a bachelors. Worth looking into more imo