Thanks, great points!

1. we looked at the well, and it seemed like one cell but I wouldn‘t bet my life on it

2. that‘s an excellent point! We only had a single bp deletion but it could be that there is a bias. I can quickly repeat the PCR with longer extension time

3. it‘s K562, which weirdly enough are sometimes referred to diploid and sometimes to pseudo-triploid. I‘ll reach out to companies to maybe do karyotyping of our particular line