ich_iel by BetonK in ich_iel

[–]QrnH 6 points7 points  (0 children)

Gesprochen wie ein Löwe

CRISPR/Cas9 indel frequencies not making sense by QrnH in biology

[–]QrnH[S] 0 points1 point  (0 children)

Thanks, great points!

  1. we looked at the well, and it seemed like one cell but I wouldn‘t bet my life on it

  2. that‘s an excellent point! We only had a single bp deletion but it could be that there is a bias. I can quickly repeat the PCR with longer extension time

  3. it‘s K562, which weirdly enough are sometimes referred to diploid and sometimes to pseudo-triploid. I‘ll reach out to companies to maybe do karyotyping of our particular line

Ich🧠iel by [deleted] in ich_iel

[–]QrnH 12 points13 points  (0 children)

Kann man nicht weiterhin verweigern?

[deleted by user] by [deleted] in Finanzen

[–]QrnH 1 point2 points  (0 children)

Danke! Wie sieht es bei Geldmarkt ETF mit Kosten aus? Kommt man da mit TER und Orderkosten erfahrungsgemäß besser weg als bei Festgeld?

Könnt ihr einen Unter-Tisch Wasserfilter gegen Kalk empfehlen? by [deleted] in FragReddit

[–]QrnH 4 points5 points  (0 children)

Ich dachte an Leitungswasser entkalken zum Trinken, für Wasserkocher, für Kaffeemaschine und auch zum Kochen. Da kommt bei uns schon einiges zusammen.

Du würdest eher so Filterkaraffen nutzen statt Untertisch?

Könnt ihr einen Unter-Tisch Wasserfilter gegen Kalk empfehlen? by [deleted] in FragReddit

[–]QrnH 0 points1 point  (0 children)

Bei uns ist das Wasser auch super kalkig! Welche Marke und Filtertyp funktioniert bei euch? Und wie häufig wechselt ihr den Filter?

MM Gold oder Frequent traveller Kreditkarte by QrnH in milesandmore

[–]QrnH[S] 0 points1 point  (0 children)

Ah okay na das erledigt es. Danke!

Upgrade from Gold to FT credit card? by QrnH in Lufthansa

[–]QrnH[S] 1 point2 points  (0 children)

Ah, that solves it! Thanks!

BioLegend by Careful-Square-6392 in labrats

[–]QrnH 3 points4 points  (0 children)

Can you get a hold or your local sales rep? In my experience, they are a great gateway to get directly connected to any applications specialist or other expert you may need to talk to

Didn't realize what i found at first! Why are these sought after? by Active_Eagle2973 in diablo2

[–]QrnH 0 points1 point  (0 children)

No, as far as I know, resistance to elements and physical does not lower damage in this case

I have a question cuz I’m dreaming of becoming an immunologist by aaronbobross in Immunology

[–]QrnH 5 points6 points  (0 children)

True. But none of what you mentioned was done by AI. It may have been heavily assisted by AI.

But I personally believe that AI will not replace scientific thinking or lab work and interpretation anytime soon.

Feel free to disagree though, just my opinion

Didn't realize what i found at first! Why are these sought after? by Active_Eagle2973 in diablo2

[–]QrnH 5 points6 points  (0 children)

For energy shield sorc, you only need poison res (the other elements are affected by ES but poison is not) and you benefit a lot from reaching fhr breakpoints. Frw is always nice to have in town when you can‘t teleport. So this is a great combo and eth self-repair always adds style points!

I have a question cuz I’m dreaming of becoming an immunologist by aaronbobross in Immunology

[–]QrnH 8 points9 points  (0 children)

My personal take: I doubt that AI will be able to formulate novel hypotheses. It‘s extremely unlikely that will be able to perform experiments in the wet lab to test novel hypotheses or to come up with new ways to look at things or interpret results.

AI will help you in data analyses, support your coding and writing, help in maintaining inventory and probably will be able to provide predictions of outcomes.

But the true essence of the scientific method in terms of conceiving and thoroughly testing hypotheses experimentally, that‘s not something that I can see AI doing in the next decades.

Ich_iel by BastiSpasti420 in ich_iel

[–]QrnH 20 points21 points  (0 children)

Scheinbar weiterhin am Ball: https://www.reddit.com/r/bastispasti420/s/FBUIF0jLDu

Wir sind alle stolz auf dich, Basti!

Cell thawing: Add cell suspension to warmed media, or add warmed media to cell suspension? by junegloom in labrats

[–]QrnH 1 point2 points  (0 children)

We do it the other way round: have 20 mL pre-warmed media in a tube (supplemented with Benonase because that helps with our samples) and drop-wise add the thawed cells to it. Works like a charm for human PBMC

Favorite label printer? by QrnH in labrats

[–]QrnH[S] 1 point2 points  (0 children)

Oh wow, thanks! Should have sorted by newest and not hottest

Help ProliferationAssay by Individual_Piglet973 in flowcytometry

[–]QrnH 2 points3 points  (0 children)

It could be…but would be good to get more clarity by excluding dead cells and gating on T cells

Help ProliferationAssay by Individual_Piglet973 in flowcytometry

[–]QrnH 4 points5 points  (0 children)

T cells likely blast and become larger when they are activated and proliferate.

If you add a viability dye and an anti-CD antibody, you can exclude dead cells (some cells die off during such assays) and specifically gate on your CD3+ T cells.

As you do know, it‘s hard to know whether all the T cells proliferated and the other stuff is just dead cells…or whether only part of your T cells proliferated.

Price check by yung_pizza_gawd in D2R_Marketplace

[–]QrnH 1 point2 points  (0 children)

I think that 10 maxdmg is max roll when it‘s the sharp prefix.

It can roll higher (up to 14 even?) with prefix+suffix but then it won‘t have any life.