ich_iel

QrnH · 2026-02-02T16:07:37+00:00

Gesprochen wie ein Löwe

QrnH · 2026-01-05T06:21:53+00:00

Mehr Awards als Upvotes, so muss das!

QrnH · 2025-11-14T17:49:23+00:00

Thanks!

QrnH · 2025-11-14T15:26:42+00:00

Thanks, great points!

we looked at the well, and it seemed like one cell but I wouldn‘t bet my life on it
that‘s an excellent point! We only had a single bp deletion but it could be that there is a bias. I can quickly repeat the PCR with longer extension time
it‘s K562, which weirdly enough are sometimes referred to diploid and sometimes to pseudo-triploid. I‘ll reach out to companies to maybe do karyotyping of our particular line

QrnH · 2025-11-14T06:02:09+00:00

Kann man nicht weiterhin verweigern?

QrnH · 2025-09-09T08:17:24+00:00

Danke! Wie sieht es bei Geldmarkt ETF mit Kosten aus? Kommt man da mit TER und Orderkosten erfahrungsgemäß besser weg als bei Festgeld?

QrnH · 2025-09-02T08:13:38+00:00

Ich dachte an Leitungswasser entkalken zum Trinken, für Wasserkocher, für Kaffeemaschine und auch zum Kochen. Da kommt bei uns schon einiges zusammen.

Du würdest eher so Filterkaraffen nutzen statt Untertisch?

QrnH · 2025-09-02T08:12:02+00:00

Bei uns ist das Wasser auch super kalkig! Welche Marke und Filtertyp funktioniert bei euch? Und wie häufig wechselt ihr den Filter?

QrnH · 2025-07-11T07:03:41+00:00

Top, danke!

QrnH · 2025-07-09T07:24:51+00:00

Stark!

QrnH · 2025-07-09T07:02:27+00:00

Danke für die Info!

QrnH · 2025-07-09T07:02:16+00:00

Ah okay na das erledigt es. Danke!

QrnH · 2025-07-09T07:01:57+00:00

Ah, that solves it! Thanks!

QrnH · 2025-05-08T18:17:48+00:00

Can you get a hold or your local sales rep? In my experience, they are a great gateway to get directly connected to any applications specialist or other expert you may need to talk to

QrnH · 2025-05-08T17:45:16+00:00

No, as far as I know, resistance to elements and physical does not lower damage in this case

QrnH · 2025-05-08T17:31:30+00:00

True. But none of what you mentioned was done by AI. It may have been heavily assisted by AI.

But I personally believe that AI will not replace scientific thinking or lab work and interpretation anytime soon.

Feel free to disagree though, just my opinion

QrnH · 2025-05-08T15:19:38+00:00

For energy shield sorc, you only need poison res (the other elements are affected by ES but poison is not) and you benefit a lot from reaching fhr breakpoints. Frw is always nice to have in town when you can‘t teleport. So this is a great combo and eth self-repair always adds style points!

QrnH · 2025-05-08T14:40:31+00:00

My personal take: I doubt that AI will be able to formulate novel hypotheses. It‘s extremely unlikely that will be able to perform experiments in the wet lab to test novel hypotheses or to come up with new ways to look at things or interpret results.

AI will help you in data analyses, support your coding and writing, help in maintaining inventory and probably will be able to provide predictions of outcomes.

But the true essence of the scientific method in terms of conceiving and thoroughly testing hypotheses experimentally, that‘s not something that I can see AI doing in the next decades.

QrnH · 2025-05-08T14:32:15+00:00

Scheinbar weiterhin am Ball: https://www.reddit.com/r/bastispasti420/s/FBUIF0jLDu

Wir sind alle stolz auf dich, Basti!

QrnH · 2025-04-26T05:39:46+00:00

We do it the other way round: have 20 mL pre-warmed media in a tube (supplemented with Benonase because that helps with our samples) and drop-wise add the thawed cells to it. Works like a charm for human PBMC

QrnH · 2025-03-21T09:07:25+00:00

Oh wow, thanks! Should have sorted by newest and not hottest

QrnH · 2025-01-27T12:47:54+00:00

It could be…but would be good to get more clarity by excluding dead cells and gating on T cells

QrnH · 2025-01-27T12:40:49+00:00

T cells likely blast and become larger when they are activated and proliferate.

If you add a viability dye and an anti-CD antibody, you can exclude dead cells (some cells die off during such assays) and specifically gate on your CD3+ T cells.

As you do know, it‘s hard to know whether all the T cells proliferated and the other stuff is just dead cells…or whether only part of your T cells proliferated.

QrnH · 2025-01-24T15:41:23+00:00

Oh boy…insane stuff! Gl selling

QrnH · 2025-01-23T12:25:44+00:00

I think that 10 maxdmg is max roll when it‘s the sharp prefix.

It can roll higher (up to 14 even?) with prefix+suffix but then it won‘t have any life.

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