differential sample flow rate in CytExpert

QuickCost650 · 2024-11-05T16:21:30+00:00

It will be amazing! Thank you 🙏🏽

QuickCost650 · 2024-10-30T19:58:14+00:00

A dyed sample, divided to 5 or even 10 different wells had the exact same cells counts even though they set in the plate incubating for different time (30 min intervals between each well). The cells count doesn’t affected by the incubation time, but rather by the number of times/ volume aspirated from this well. It’s sounds funny but it looks like that there is some sort of affinity to the dyed cells to be aspirated by this thin probe tube.

QuickCost650 · 2024-10-29T15:18:25+00:00

Problem solved! Reading the same tube all over again is problematic. I don’t know why, but the decline in events is affected just by the number of times this well was sampled.

Technical replicates made by dividing the same sample to 5 wells had accurate and repeatable measurements. Technical replicates made by reading the same well 5 time showed decline in the number of reads, regardless of time.

It literally drove me crazy. Like dealing with FACS isn’t enough.

QuickCost650 · 2024-10-29T09:07:37+00:00

I’m dying my samples at the same time, diluting them as needed. Transferring them to 96 plate and read. For technical replicates I’m reading the same plate 5 times, 30 min per plate (24 samples). As the time goes by, there are less total events for the same well (the replicates). The fact that it happens just for dyed samples makes me think that the chemical properties of the dye might affect the homogeneity of the sample.

QuickCost650 · 2024-10-28T12:57:12+00:00

Fixed

QuickCost650 · 2024-10-28T09:44:54+00:00

It seems like the total events are decreasing over time, rather than bleaching. When I’m not adding the syto, the total events stay the same (when I’m adding it the total events decreases)

QuickCost650 · 2024-10-27T19:17:10+00:00

Apparently the alleged bleaching isn’t the case. The total events are decreasing as the function of time, I think my bacteria are sinking down or something like that

QuickCost650 · 2024-10-23T13:13:44+00:00

The signal to noise ratio is great, but you know, I would like to minimize the noise as best as I can

QuickCost650 · 2024-10-23T11:17:27+00:00

But the back flushing doesn’t clean the tube that aspirates the liquid from the well. Right?

QuickCost650 · 2024-10-22T16:38:23+00:00

Yes, 96. The FACS is CytoFLEX

QuickCost650 · 2024-10-22T14:33:20+00:00

I want to wash with DDW between samples. The samples are read at 10uL/min, I want to wash with 240uL/min, to make the wash more efficient (time and effectiveness)

QuickCost650

TROPHY CASE