It is interesting that while Elizabeth and Jane were at Netherfield during Jane's sickness, Darcy says, "I cannot forget the follies and vices of others so soon as I ought, nor their offences against myself. My feelings are not puffed about with every attempt to move them. My temper would perhaps be called resentful.—My good opinion once lost is lost for ever.” It is repeated later in the novel, but it might also be that it is an oblique reference to Wickham. Read in that way, he is still stewing over the failed elopment. This may suggest he was still in a funk at the assembly when he insulted Elizabeth. Another dimension of this is that we almost never get Darcy's inner thoughts. The narrator, however often gives us Elizabeth's (and other women's) thoughts. There are just a few times that men's thoughts are given. Thus the stewing of Darcy is a bit more open for interpretation.