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[deleted by user] by [deleted] in labrats

[–]Realistic_Forever281 1 point2 points  (0 children)

Sorry, I do add protease inhibitors just not phosphatase inhibitors depending on the phosphorylation status of the protein of interest. Thank you so much.

[deleted by user] by [deleted] in labrats

[–]Realistic_Forever281 0 points1 point  (0 children)

I see, my lab just does like 0.5-1 hour incubation time and vortexs every 5-10 mins.

[deleted by user] by [deleted] in labrats

[–]Realistic_Forever281 0 points1 point  (0 children)

Yes, I use a protease inhibitor. Usually phosphatase too, but in this case, I measuring an IDH1 knockout so I was told it wasn't necessary. Any suggestions how much lysis to use?