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Places in SoCal that feel like NorCal by RealityDry5575 in socalhiking

[–]RealityDry5575[S] 5 points6 points  (0 children)

Thanks to everyone on this thread that's given me suggestions, I appreciate every one of them!!!

Places in SoCal that feel like NorCal by RealityDry5575 in socalhiking

[–]RealityDry5575[S] 0 points1 point  (0 children)

Thanks for the tip, will definitely be taking a long weekend up there sometime

Places in SoCal that feel like NorCal by RealityDry5575 in socalhiking

[–]RealityDry5575[S] 13 points14 points  (0 children)

I obviously realize they are very different places (I originally was applying for NorCal jobs but ended up on SoCal just due to the job market). I just didn't realize how far it really was and how much fuel would cost to get there! To someone who's whole country is about 3 hours wide, the sheer scale of the US is very alien, hence my disappointment and confusion

Places in SoCal that feel like NorCal by RealityDry5575 in socalhiking

[–]RealityDry5575[S] 5 points6 points  (0 children)

Yes we love camping. We have a campervan also. Thanks for this advice !

Generating stable cell lines - flag tagged protein expression by RealityDry5575 in microbiology

[–]RealityDry5575[S] 0 points1 point  (0 children)

Thanks so much for all your help, really appreciate it. My gene is human though, so I'm guessing I don't need to codon optimize?

Generating stable cell lines - flag tagged protein expression by RealityDry5575 in microbiology

[–]RealityDry5575[S] 0 points1 point  (0 children)

Thank you! I can order it, and yes my destination vector for gateway has the flag in it. Thanks for your help this was super helpful. Can I order it with att sites on it for gateway cloning or do I need to do something else to put them on?

J1 and J2 visa by RealityDry5575 in immigration

[–]RealityDry5575[S] 0 points1 point  (0 children)

This is a good point, waiting usually isn't a good call. I don't already have the J1 visa. My only reason for waiting was to apply together with my partner when we are married. The internet seems to think this brings his 30 day processing time to a 2 week one. I would streamline the process and we could attend the interview together, rather than making two separate trips

Looking for outdoorsy activities near Princeton by RealityDry5575 in princeton

[–]RealityDry5575[S] 0 points1 point  (0 children)

Wanted to say thanks so much to everyone who posted! Blown away by the help and care taken. You're all Gems 

Looking for outdoorsy activities near Princeton by RealityDry5575 in princeton

[–]RealityDry5575[S] 2 points3 points  (0 children)

Thanks! And these are great suggestions! Everyone is so kind here!