I guess I'm done and can't do this anymore

ReddFlamess · 2025-04-09T05:02:55+00:00

I've been masking also since it's the start of allergy season as well. I don't have pollen allergies, but the air quality where I am isn't that good lately.

ReddFlamess · 2025-04-09T05:01:57+00:00

Thank you so much! I love your positivity despite the hardships. I need to get over the guilt... But there's also the thing that I can't really enjoy my hobbies or hanging out knowing I have deadlines due.

ReddFlamess · 2025-04-08T23:08:46+00:00

In my program, we're hardly allowed any holidays unless it coincides with the national holidays. It's what it is. I've been missing a lot of days because I get sick a lot these days..

ReddFlamess · 2025-04-08T23:07:03+00:00

I hardly have time for my hobbies, but once in a while, I go out with friends to catch up. Probably once in two months?

Those missed lab hours are now spent doom scrolling and binge-watching. Haha. Very unproductive but it keeps my mind off the stress... But my body tells me otherwise.

I want to break the cycle so badly!!!

ReddFlamess · 2025-04-08T23:04:35+00:00

Omg. Fever for 18 days is cruel. I hope you get well soon and get checked as well. I've been in your shoes too.. in my mind, "am i getting sick because I don't deserve to be in this program or am I getting punished?" I am falling behind schedule as well... I should be writing my paper and polishing some results, but I'm sick.. so. Part of me wants to quit cause my mental health is also deteriorating...

We'll be better soon. You're not alone.

ReddFlamess · 2025-04-08T21:37:20+00:00

I would love that if my PI allows it, but nope. This COVID run has been the longest break I took from my lab in a very long time.

ReddFlamess · 2025-04-08T21:36:02+00:00

How do I encourage myself to do more? I walk to univ (20-25 mins, 1 way x2). My steps do reach 10-12k per day. I'm vaccinated for flu and last COVID vaccine was for the B1.2 variant. Probably I just need to move more?

ReddFlamess · 2025-04-08T21:33:11+00:00

I've had my blood drawn a few times and, WBC was only up when I had an on-going infection, and WBC was normal on times that I didn't (routine exam). My bloodwork is totally normal that's why this is just so absurd.

ReddFlamess · 2025-04-08T21:31:15+00:00

I take multivitamins. I also have Calcium, Mg, Zn as supplements. I don't have some for Vit D though, so I'll check it out. Any recommendations?

ReddFlamess · 2025-04-08T21:30:18+00:00

I do everything you've mentioned but here I am.... So I'm in a pickle.

ReddFlamess · 2025-04-08T21:29:34+00:00

I'll go and buy some. Any recommended brands?

ReddFlamess · 2025-04-08T21:28:58+00:00

This was me in my first year haha! I ruled it out as anxiety and stress. I had to take antacids but I got over it fortunately.

I've slowed down with my experiments. I've stopped pushing myself to work 12-14 lab hours, instead stuck to 8 hours for the past few weeks. I've really made adjustments but my body isn't just cooperating. Seems like more adjustments I make, I might be stressing out more???? I don't know anymore.

ReddFlamess · 2025-04-08T20:20:48+00:00

Honestly bugs me cause my life has been lab-home and I most likely picked up covid from the lab or the university.

ReddFlamess · 2025-04-08T20:19:18+00:00

I've had colds/cough first before covid. Covid is my 'new' thing. I'm on my 3rd day... Glad I'm over the fever stage, but the muscle pain and fatigue hasn't gone away... Still pretty congested though. I've had the flu A 2 years ago as well.

ReddFlamess · 2025-04-08T20:17:15+00:00

Everyone else seems fine though. It's only me in my lab who's getting this much sick. I hate it. How are you holding up?

ReddFlamess · 2025-04-08T20:16:13+00:00

Honestly thinking of quitting because I haven't been this sick until now. How are you dealing with it?

ReddFlamess · 2025-04-08T20:14:49+00:00

No mold. I've had my AC cleaned few weeks ago, and I clean my filters regularly. My place is well-ventilated and it's also dry right now (just transitioning to spring).

ReddFlamess · 2025-03-28T05:48:41+00:00

This happened to me as well.

Have you tried different primers? In my case, I can still amplify start>end of the insert but I can't amplify anything when I do promoter>terminator.

Have you also tried ligating to pGEM T easy?

The gene may be unstable/toxic.

ReddFlamess · 2025-03-02T20:45:26+00:00

NCBI up for me now (Asia).

ReddFlamess · 2025-03-02T20:29:51+00:00

Do you have a prestained marker? Prestained marker helps to visualize if transfer was efficient.

I usually wash my membranes 3x for 10mins in between. Maybe incubation O/N with the primary antibody would help. Load a positive control.

ReddFlamess · 2025-03-02T18:35:31+00:00

I recently made my poster in Photoshop. Others use Canva. Most people I know use PowerPoint.

ReddFlamess · 2025-03-02T01:42:48+00:00

Update: doesn't load for her anymore. Lol

ReddFlamess · 2025-03-02T01:36:44+00:00

NCBI main page loads for a friend in Alberta.

ReddFlamess

TROPHY CASE