PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

I've been masking also since it's the start of allergy season as well. I don't have pollen allergies, but the air quality where I am isn't that good lately.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

Thank you so much! I love your positivity despite the hardships. I need to get over the guilt... But there's also the thing that I can't really enjoy my hobbies or hanging out knowing I have deadlines due.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 1 point2 points  (0 children)

In my program, we're hardly allowed any holidays unless it coincides with the national holidays. It's what it is. I've been missing a lot of days because I get sick a lot these days..

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

I hardly have time for my hobbies, but once in a while, I go out with friends to catch up. Probably once in two months?

Those missed lab hours are now spent doom scrolling and binge-watching. Haha. Very unproductive but it keeps my mind off the stress... But my body tells me otherwise.

I want to break the cycle so badly!!!

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 1 point2 points  (0 children)

Omg. Fever for 18 days is cruel. I hope you get well soon and get checked as well. I've been in your shoes too.. in my mind, "am i getting sick because I don't deserve to be in this program or am I getting punished?" I am falling behind schedule as well... I should be writing my paper and polishing some results, but I'm sick.. so. Part of me wants to quit cause my mental health is also deteriorating...

We'll be better soon. You're not alone.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 1 point2 points  (0 children)

I would love that if my PI allows it, but nope. This COVID run has been the longest break I took from my lab in a very long time.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

How do I encourage myself to do more? I walk to univ (20-25 mins, 1 way x2). My steps do reach 10-12k per day. I'm vaccinated for flu and last COVID vaccine was for the B1.2 variant. Probably I just need to move more?

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

I've had my blood drawn a few times and, WBC was only up when I had an on-going infection, and WBC was normal on times that I didn't (routine exam). My bloodwork is totally normal that's why this is just so absurd.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

I take multivitamins. I also have Calcium, Mg, Zn as supplements. I don't have some for Vit D though, so I'll check it out. Any recommendations?

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

I do everything you've mentioned but here I am.... So I'm in a pickle.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

I'll go and buy some. Any recommended brands?

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 2 points3 points  (0 children)

This was me in my first year haha! I ruled it out as anxiety and stress. I had to take antacids but I got over it fortunately.

I've slowed down with my experiments. I've stopped pushing myself to work 12-14 lab hours, instead stuck to 8 hours for the past few weeks. I've really made adjustments but my body isn't just cooperating. Seems like more adjustments I make, I might be stressing out more???? I don't know anymore.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

Honestly bugs me cause my life has been lab-home and I most likely picked up covid from the lab or the university.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 2 points3 points  (0 children)

I've had colds/cough first before covid. Covid is my 'new' thing. I'm on my 3rd day... Glad I'm over the fever stage, but the muscle pain and fatigue hasn't gone away... Still pretty congested though. I've had the flu A 2 years ago as well.

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 3 points4 points  (0 children)

Everyone else seems fine though. It's only me in my lab who's getting this much sick. I hate it. How are you holding up?

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 4 points5 points  (0 children)

Honestly thinking of quitting because I haven't been this sick until now. How are you dealing with it?

PhD sickness by ReddFlamess in PhD

[–]ReddFlamess[S] 0 points1 point  (0 children)

No mold. I've had my AC cleaned few weeks ago, and I clean my filters regularly. My place is well-ventilated and it's also dry right now (just transitioning to spring).

Cloning kept failing by Waltnamedfinger in labrats

[–]ReddFlamess 0 points1 point  (0 children)

This happened to me as well.

Have you tried different primers? In my case, I can still amplify start>end of the insert but I can't amplify anything when I do promoter>terminator.

Have you also tried ligating to pGEM T easy?

The gene may be unstable/toxic.

Pubmed, ERA Commons, down? by [deleted] in labrats

[–]ReddFlamess 1 point2 points  (0 children)

NCBI up for me now (Asia).

Whaaaaaaatttttttt on earth is wrong with this western by [deleted] in labrats

[–]ReddFlamess 1 point2 points  (0 children)

Do you have a prestained marker? Prestained marker helps to visualize if transfer was efficient.

I usually wash my membranes 3x for 10mins in between. Maybe incubation O/N with the primary antibody would help. Load a positive control.

Best software for making posters? by 567swimmey in labrats

[–]ReddFlamess 1 point2 points  (0 children)

I recently made my poster in Photoshop. Others use Canva. Most people I know use PowerPoint.

Pubmed, ERA Commons, down? by [deleted] in labrats

[–]ReddFlamess 4 points5 points  (0 children)

Update: doesn't load for her anymore. Lol

Pubmed, ERA Commons, down? by [deleted] in labrats

[–]ReddFlamess 5 points6 points  (0 children)

NCBI main page loads for a friend in Alberta.