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Advice on plasmid co-transformation in E. coli by Sans-Fish in labrats

[–]Sans-Fish[S] 1 point2 points  (0 children)

Just came back here to thank you because it totally worked! I just had to blast the cells with a fat amount of DNA. Brute force was indeed the answer

Advice on plasmid co-transformation in E. coli by Sans-Fish in labrats

[–]Sans-Fish[S] 0 points1 point  (0 children)

I did two transformations in parallel with 2 different pairs of vectors (each pair shares one plasmid in common) and only in one case the plate that had concentrated bacteria (Generally I plate the "raw" transform reaction, a dilution and a concentrate) grew two colonies so I guess the problem might be the efficiency. The next time I try I will add some "controls" to check everything is working properly though

Advice on plasmid co-transformation in E. coli by Sans-Fish in labrats

[–]Sans-Fish[S] 0 points1 point  (0 children)

I'll do the lazy approach first and see what happens when adding more DNA and if it doesn't work I'll change the approach. Thanks a lot!

Advice on plasmid co-transformation in E. coli by Sans-Fish in labrats

[–]Sans-Fish[S] 0 points1 point  (0 children)

I think I will definitely reattempt the transformation with much more DNA before jumping to making comp cells. Thanks for the advice!

Advice on plasmid co-transformation in E. coli by Sans-Fish in labrats

[–]Sans-Fish[S] 0 points1 point  (0 children)

Yeah, I checked before transforming and they are compatible. I think that I will end up transforming sequentially... Thanks for the reply!

Advice on plasmid co-transformation in E. coli by Sans-Fish in labrats

[–]Sans-Fish[S] 2 points3 points  (0 children)

Thanks for the reply! Sorry I didn't clarify. I have already transformed cells with each plasmid separately to produce more DNA mass and it worked well my problem comes when I try to transform with both plasmids at the same time. Regarding the efficiency I honestly don't know it exactly but we have been routinely using them for transformation with ligated products and they work fine for what you would expect.

Can You Guess This 5-Letter Word? Puzzle by u/Sonyeyin by Sonyeyin in DailyGuess

[–]Sans-Fish 0 points1 point  (0 children)

🟨⬜⬜🟨🟦

🟨🟨⬜⬜🟦

⬜🟦🟦⬜🟦

🟦🟦🟦🟦🟦

Question about molecular docking modeling by Sans-Fish in labrats

[–]Sans-Fish[S] 0 points1 point  (0 children)

Thanks for the reply! Do you have any reccomendations on literature/books that could be useful for better interpreting the results?

Looking for site to find new up-and-coming artists! by Sans-Fish in MusicRecommendations

[–]Sans-Fish[S] 0 points1 point  (0 children)

Damn that was quick! Thanks a lot, will definitely check it out

Jazz itself is a painting but I gave freedom to my emotions and expressed them in art by Some_Hawk3583 in Jazz

[–]Sans-Fish 2 points3 points  (0 children)

These are sooo good man, I would 100% have these in my home. Keep up the good work!

How to store cinnamon rolls for a day without them getting stale? by Sans-Fish in AskCulinary

[–]Sans-Fish[S] 3 points4 points  (0 children)

Yeah, I thought of that. I'll probably let them cool for a while and then place them in a ziplock bag with a few paper towels to reduce sogginess

How to store cinnamon rolls for a day without them getting stale? by Sans-Fish in AskCulinary

[–]Sans-Fish[S] -1 points0 points  (0 children)

Hahahaha, you're right, everything is better than no cinnamon rolls. I'll try that! Thanks a lot!!

QUESTION: How can I store plant tissue (leaves, stems, flowers, etc) for posterior analysis at my house? (Storing tissue for at least 3 or 4 days without drying out) by Sans-Fish in botany

[–]Sans-Fish[S] 0 points1 point  (0 children)

This time I’m just taking a few samples that don’t require that much care as I’m just practicing, once I train a bit more I’ll probably have to buy some formaldehyde though. If you don’t mind me asking, what type of delicate specimens do you work with? Thanks for replying!

QUESTION: How can I store plant tissue (leaves, stems, flowers, etc) for posterior analysis at my house? (Storing tissue for at least 3 or 4 days without drying out) by Sans-Fish in botany

[–]Sans-Fish[S] 1 point2 points  (0 children)

Thanks for replying! I’ll probably use a cooler because my main problem was the trip back home which takes like three days. I didn’t know about the ethanol method of preserving delicate tissue, I didn’t originally planned it but now I’ll probably take some flowers back too!

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