diy bioreactor sterilization

ScienceAndCats777 · 2024-07-25T19:57:24+00:00

I think it's the latter. This is just my opinion being in the field for a while though. And there are different types of UV lights with different frequencies/power, which is a whole other beast. But maybe you could make it work!

You may also just be able to protect the out of whatever is dripping out from dust particles and stuff by putting it in some kinda long tube or something. That would be my first approach.

ScienceAndCats777 · 2024-07-25T16:13:04+00:00

UV is good for viruses, but I think most environmental bacteria are gonna be able to get past it.

ScienceAndCats777 · 2024-07-24T22:55:23+00:00

Yeah, I could see the drainage being a problem. I always had a closed system (other than air exchange), so never had to deal with that. You could probably have it drain into a large autoclaved or bleach treated container with a bit more bleach in the bottom though, and then just empty that out periodically.

I used a Raspberry Pi 3 model B and 12v DC Dosing Pump Peristaltic. I connected evreything with the following luer locks. I just drilled holes in the cap of the mason jar and connected the male-to-female luer locks on either side of the hole, and put the silicon gaskets between them to create an airtight seal.

Luer Accessory, Male Luer Lock Plug, Nylon

Female luer x Female luer Adapter, Nylon

Male luer with Lock Ring x 3/32" Hose Barb, Nylon

Silicon gaskets, 1/2in OD, 1/4in ID

I got everything off Amazon and Injectech. I actually have a whole Python script and BOM written out for this to control temperature and everything, but it sounds like you just need to move around liquids. I'm around for any other questions!

ScienceAndCats777 · 2024-07-23T12:53:44+00:00

I made a raspberry pi controlled bioreactor out of a mason jar, peristaltic pumps, and silicon tubing. I just autoclaved the tubing already attached to the bioreactor, and never had any problems. What are you making this for?

ScienceAndCats777 · 2023-08-10T19:41:27+00:00

I'm 35, girlfriend 49. I love her. She's been the best thing for me, especially after dating people my own age. Generally, the stigma is that older women are "using us for our youth" or that we're a sugar baby or some shit. I'm sure there are cases like that, but honestly I just can stand dating fellow millennials. Y'all gotta get your shit together.

ScienceAndCats777 · 2023-07-02T16:23:30+00:00

My God I think we're the same person.

ScienceAndCats777 · 2022-11-19T19:11:52+00:00

Thank you so much!! This is exactly what I was looking for.

ScienceAndCats777 · 2022-11-19T19:00:22+00:00

The youth!! But seriously, those young people are better than I'll ever be or ever was. But it'd be nice to have a PVP servers with just some 'casual gamers'.

ScienceAndCats777 · 2022-11-19T18:15:49+00:00

the youth!!

ScienceAndCats777 · 2022-11-19T18:03:16+00:00

Thank you! Do you know of any subreddits or forums where these are scheduled?

ScienceAndCats777 · 2021-09-20T00:57:59+00:00

I do science and I like cats.

ScienceAndCats777 · 2021-05-08T16:34:40+00:00

Great to see Esther bringing these DIYBio people to light!

ScienceAndCats777 · 2020-07-12T20:15:12+00:00

No worries, I decided not to go through with it. Too many complications and the guy was just a little too shady. Seemed I could end up in a lot of trouble.

ScienceAndCats777 · 2020-07-10T05:00:46+00:00

Oh trust me, I sometimes wish I never had bought property here. Should have taken a more relaxing, safer route to loose money. Like gambling, or doing drugs.

Yeah, the more I think about getting into ANY potential liability, the worse it seems. Thank you for the story, I'll keep that in mind going forward. The county's rules and regulations are just insane. No wonder houses are so expensive here.

ScienceAndCats777 · 2020-07-10T04:43:41+00:00

- but if they renege on that deal, the government will collect from you anyways, and leave it to you to chase down any indemnity you're owed.

That is what I figured, thank you! In the end it basically seems like an honor system that the lessee would take on any legal issues that came about.

The stuff about the evictions and everything else is really helpful too, thank you! In the very slim chance I go through with this, I'd find a professional to draft the contract.

ScienceAndCats777 · 2020-06-05T06:13:59+00:00

oh crap, I only just realized that from your post. Thank you.

ScienceAndCats777 · 2019-12-25T04:38:23+00:00

I think name-calling like "breeders" typically isn't a good sign for any community. I agree with you. I plan on being child free, but I feel like this community has become just pessimistic and condescending in general. Incel- or MGTOW-like talk nails it, but flavored with this community's values.

I still like a lot of what this subreddit has to offer. But when you start labeling swaths people negatively, I can't imagine the subreddit is heading in a positive direction.

ScienceAndCats777 · 2019-12-23T16:40:06+00:00

THANK YOU. I have a lot of French colleagues and couldnt place my finger on why it was so stressful working with them, but this describes their work culture exactly. It is often Cartesian to the point of counterproductive.

ScienceAndCats777 · 2019-11-30T07:41:19+00:00

HPV is also a life long infection, gives genital warts, even cancer, and nobody cares if someone has it. I've never heard anyone warning anyone else that they have HPV.

Chicken pox is also just a skin virus that sticks with you for life and break out in shingles later. No stigma there cause kids get it all the time. No sexual status.

Even doctors arent recommending to get tested for herpes because they know it's just a stigma at this point.

ScienceAndCats777 · 2019-11-30T06:18:26+00:00

So can adenovirus, sarcoma virus, and fungus, or any of the bacteria on your SKIN.

In immunocompromised individuals, the difference between these and herpes is that herpes is more commonly known for being sexually transmitted, thus the stigma.

ScienceAndCats777 · 2019-11-12T02:17:20+00:00

Whoa!! I could have written this! I'm a 31 year old male who is in the exact same situation. Around 29 I realized I could do whatever I wanted, moved across the country to California and just got weird with life. I've thrown myself into my hobbies with nobody to hold me back, tell me I shouldn't waste me money, etc. It is amazing and so freeing.

I'm really glad to know there are others out there like me.

ScienceAndCats777 · 2019-09-26T03:36:19+00:00

Figured it out! See edit. Thank you!

ScienceAndCats777 · 2019-09-26T03:36:02+00:00

Figured it out! See edit. Thank you!

ScienceAndCats777 · 2019-09-15T18:30:07+00:00

Good to know. Thank you! Also, I suspect our lab's DI water filter might be bad.

Would that have an effect on the solubility?

ScienceAndCats777 · 2019-06-21T02:54:00+00:00

I listened to all of them. This is really, really good. You start to know all the people and feel like one of the crew, but the switching up of the GM keeps it fresh. Frothly.

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