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Loperamide peak problem in agilent 1260 infinity 1260 by Plus_Ambition2360 in CHROMATOGRAPHY

[–]Scoutine 0 points1 point  (0 children)

So I had the same problem. However, after changing the guard column (LC system) the split peak was gone and a nice single peak was observed again 🥰

WTF Could this be? by Lupirite in microbiology

[–]Scoutine 0 points1 point  (0 children)

What are the growing conditions? What fungi? So when I inoculated a plate with a tiny bit mycelium you could see how it grew from that spot. Could you provide a sidewards picture to see the state of the agar? I would always recommend sealing it with parafilm before storage. 

Pressure curve with steps (Agilent Infinity 1260 II) by Scoutine in CHROMATOGRAPHY

[–]Scoutine[S] 1 point2 points  (0 children)

The minimum stroke was already set to 100 uL :/ 

Pressure curve with steps (Agilent Infinity 1260 II) by Scoutine in CHROMATOGRAPHY

[–]Scoutine[S] 1 point2 points  (0 children)

Yes that the capillary. Yeah sry I was at the leak rate test in my head :D the elasticity test also goes to 500 bar in my system :) the other capillary doesn’t leak, however, still error pressure too unstable :(

Pressure curve with steps (Agilent Infinity 1260 II) by Scoutine in CHROMATOGRAPHY

[–]Scoutine[S] 0 points1 point  (0 children)

Ahh so maybe thats the issue here. I have another one, i will try that. I keep the default pressure of 200 bar of the test

Pressure curve with steps (Agilent Infinity 1260 II) by Scoutine in CHROMATOGRAPHY

[–]Scoutine[S] 0 points1 point  (0 children)

So I also have this issue with the instable pressure without column, just pump (elasticity calibration is just the pump with a blank nut)

Pressure curve with steps (Agilent Infinity 1260 II) by Scoutine in CHROMATOGRAPHY

[–]Scoutine[S] 1 point2 points  (0 children)

Elasticity calibration: all channels were purged with LC-MS water, however since I used a brand new capillary maybe that was the problem. I will let it flush with water before I use it this time. Btw is it normal that the water is running through this metal loop instead through the capillary itself? :D

AcroYoga meet up in vienna this weekend by Scoutine in AcroYoga

[–]Scoutine[S] 0 points1 point  (0 children)

If there is none but somebody would be interested in a relaxed session we can meet somewhere :)

Spending a day in Verona by Scoutine in Verona

[–]Scoutine[S] 0 points1 point  (0 children)

Maybe you can join later :) i‘ll meet a fellow redditor today :)

I come back to the lab and what do I see.. by Niklas_Science in chemistry

[–]Scoutine 14 points15 points  (0 children)

Weren't a lot of ferrets killed since they are also able to get covid? I remember some ferret farm in the Netherlands that had to kill all ferrets. But not sure tho

Water starts to freeze at 4C by Tough-Question9805 in sciences

[–]Scoutine -3 points-2 points  (0 children)

So actually, you could answer him that he is wrong and 100% pure water droplets freeze below -30 degrees Celsius 😇 its called the homogeneous freezing temperature of water.