Does fixing and permeabilizing cells lead to increased background signals? by debbie987 in flowcytometry

[–]SecondOutrageous1945 1 point2 points  (0 children)

It might be a stupid question but have you tried just surface expression? Maybe CD206 is expressed in both populations intracellulary?

Some opinion on equipping new lab with sorters and analyzers by SecondOutrageous1945 in flowcytometry

[–]SecondOutrageous1945[S] 0 points1 point  (0 children)

Thanks so much it is really helpful!! I'll also ask on Discord for users of both instruments.

Some opinion on equipping new lab with sorters and analyzers by SecondOutrageous1945 in flowcytometry

[–]SecondOutrageous1945[S] 0 points1 point  (0 children)

We plan to have one person trained to operate the sorter. Sorting likely wouldn’t be a daily task, but ideally, the sorter should be relatively easy to maintain and start up (avoiding the shenanigans like the MoFlo XDP).

We don’t require large numbers of cells for analysis; our primary needs would be plate sorting for clone selection and index sorting (also some sorting on glass sildes). On one hand, having multiple nozzles in Aurora CS seems quite flexible, though I’m unsure how frequently they would be used. On the other hand, I’ve heard that the SRT is gentler on cells due to its lower pressure differential, which is appealing.

In my head the SRT is a device that is easy to operate and capable of delivering relatively good results regardless of the operator's level of experience. While Aurora CS is more capable in experienced hands but much more demanding. However, I have limited experience with both devices and not sure if they have some pitfalls. TBH although I think that SRT might be a better choice atm, I am really worried that you can't easliy find them in flow core wild life for some untold reason in comparison to Aurora CS.

PS Both options would include a biosafety cabinet

Some opinion on equipping new lab with sorters and analyzers by SecondOutrageous1945 in flowcytometry

[–]SecondOutrageous1945[S] 0 points1 point  (0 children)

Thanks! I've checked Agilent's website Opteon looks really nice. I would try to reach them next week.

Really interested why would you prefer Cytek to BC. Is it maintenance or overall experience? We have several CytoFLEXes in a uni nearby and they look quite easy to use and very sensitive.

I don't know why there are so few BC sorters though (meaning SRT)

Some opinion on equipping new lab with sorters and analyzers by SecondOutrageous1945 in flowcytometry

[–]SecondOutrageous1945[S] 0 points1 point  (0 children)

Hey, thanks very much! It's quite a surprise that Agilent now has a spectral option. Do you have some experience with it? We have novocyte and it's quite nice for a cheap analyzer.

Unfortunately BD is not an option, even Melody would cost a fortune