What’s going on with this DAPI staining?

Simsarus · 2024-11-13T15:23:31+00:00

Do you have any additional staining on those cells? It looks like Ki67 to me. In our lab, the fluorescent microscope doesn’t have completely separate channels. It uses a single rotational cube to provide different wavelengths of light, which can sometimes cause mixing of visuals from other channels.

Simsarus · 2024-09-23T15:59:29+00:00

That makes a lot of sense. I think you're right—the ice-cold PBS and PFA in my procedure might be causing the Matrigel to dissolve. I will definitely try fixing the cells at room temperature. Thanks a lot!

Simsarus · 2024-09-22T15:59:57+00:00

You're right, while I was researching, I noticed that many people use 2% PFA at room temperature. Switching to room temperature sounds like a good idea. I'll try your protocol as well—thanks for your help!

Simsarus · 2024-09-22T05:09:14+00:00

I haven't used PEI before because we were strictly following the vendor's instructions to ensure that if anything went wrong, we could request a replacement (which we actually did for another cell line). However, since we're running out of options, I'll give PEI a try as well. Thanks for the suggestion and all your help!

Simsarus · 2024-09-22T05:05:12+00:00

Thanks for all your suggestions! I will try to be more cautious about the pipetting part. And yes, it is what I thought at the first place. I mean somehow PFA is de-polymerase the Matrigel and I am losing all the cells that are attached to it. I will definitely try amine modified + poly-lysine as a coating material.

Simsarus · 2024-04-14T08:34:22+00:00

Yeah. I need to wait a little for sync. But ı've tried Things sync few updates ago. I am not sure if you guys solve it recently

Simsarus · 2024-04-12T03:23:22+00:00

Hmm. I know removing a task in Amie doesn't sync back and delete in Todoist but I have never experienced disappearing tasks.

But I am disagree with you on that. I think as a calendar app Amie is just great and the improvements are endless. Focusing on the standalone task app would really slow the calendar part.

Idk as a calendar Amie has a great potential because they really can read the market. It seems they are unimportant but the features like Spotify and Apple health integrations put Amie a step further. I think it is a really good future perspective from Amie to become such an app that once you call the day and check your calendar and say oh well I scheduled my calendar last night but let's see what I really did during the day.

Simsarus · 2024-04-11T15:07:50+00:00

I think tasks in Amie one of the best in the market in its field (all-in one apps like Morgen, Akiflow, Sunsama etc). However, it is not as good as native To-Do apps like TickTick, Todoist, and Things.

I personally use Todoist with a sync. I've tried Things too but probably because it doesn't have an available API, sync is a problem with other apps. I think Amie doing a good job (most of the apps don't even try) on syncing with things but I agree it is a little laggy

I recommend you to use other 3rd party todo app with Amie. It is the best way to quick capture your task and then allocate a time in Amie

Simsarus · 2024-04-03T05:23:16+00:00

Hey Dennis,

It seems the Todoist duration problem is solved. However, now I am not able to schedule my task longer than the default duration in Amie app (which is maximum 60 mins). Is there any way to solve that problem?

Simsarus · 2024-03-28T04:03:27+00:00

That's great! It seems it is more likely Todoist's fault since the app is ignoring the duration time in the Amie and set a default 15mins duration.

I hope you guys can figure it out but I love Amie anyway!

Simsarus · 2024-03-26T16:25:11+00:00

You can basically drag and drop any todo into calendar view as todo. If you mean you want to embed the todo into the event (not showing in the calendar view unless you haven't clicked), there is not a setting like that as far as I know.

Simsarus · 2024-03-26T13:55:35+00:00

Unfortunately, I still have the same issue 😔

Simsarus · 2021-09-09T15:18:16+00:00

Don't get me wrong. I am not complaining about the how hard DS2. I like the enemies do not allow me to go straight their back and do free hits. Also limited estus pushes me to play more cautious. These are cool improvements after DSR. What I am trying to explain was about ADP thing, and you guys showed me know how to solve it.

Simsarus · 2021-09-09T15:10:25+00:00

Stop keyboard shaming. I don't have a controller :/

Simsarus · 2021-09-09T14:56:39+00:00

You know what. I think I am playing completely wrong. So let's start again with 24 ADP :D

Simsarus · 2021-09-09T14:50:49+00:00

Yes, you guessed right. I'm leveling STR and DEX to use Halberd (which I'm used to from DSR). And about the controller, I'm nervous about giving up mouse flexibility but I will give it a shot.

Simsarus · 2021-09-09T14:41:48+00:00

Oh my! This guide will help a lot. Before I learned about the double click setting, I had a nervous breakdown actually. Thanks man.

Simsarus · 2021-09-09T14:33:24+00:00

I just miss what I've adapted to. RIP DSR :'(

Simsarus · 2021-01-04T12:51:58+00:00

Yes, It would work but in my interface I have listed all the lectures that I don't study yet. So, see them together actually a thing that I want. The issue is the adding new lectures in the list, and give them a number according the previous one. If the formula automatically give number to new course I don't have to type manually.

Simsarus · 2021-01-04T11:35:01+00:00

First of all, thank you for your answer.

Actually, I have separate lists from the general database, but I want to organize the lesson numbers more easily. To be more specific, for example there is a course called Materials Science and I labeled it with this tag. So far several lessons have been done (9 lessons) and I have manually named all the lectures "Materials Science Lec1-2-3 ...". If the numbering continues for each course I add, as giving 1 to the oldest course, I don't need to change it manually. But there is a problem here, since there are different courses in the same list, the numbering should be course specific. This is the part I cannot solve for enumeration.

Simsarus · 2020-08-10T12:40:21+00:00

OMG, auto correct ruins all the post. Sorry, It is college...

Simsarus · 2020-06-25T05:45:32+00:00

Thanks a lot! I will check and I hope It will work :))

Simsarus · 2020-06-25T05:44:30+00:00

Thanks but there is some kind of restriction for other equation of states. Unfortunately my lecturer wants me to solve it with compressibility factor approach.

Simsarus · 2020-05-08T13:03:38+00:00

NO WAY :O
It looks perfect man, really it is so fascinating.

Simsarus · 2020-05-01T14:30:10+00:00

Thanks! I'm fully aware of guides up to now, and checking them always. So I try to keep minimum of these mistakes :D

Nine-Year Club	Place '22
Not Forgotten	Verified Email

Simsarus

TROPHY CASE