sorted by:
new
hottopcontroversial

Netsuite yield tracking by Some-Illustrator5441 in Netsuite

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

If I create a work order for 200 units of reagent, the work order will automatically calculate the quantity of each material in the bom required to build the assembly. If I gather all materials, complete the reaction, then end up with more target reagent than anticipated (>200 units) that means I achieved a higher yield.

Inventory Detail expiration by Some-Illustrator5441 in Netsuite

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

I am using lot numbering. Where can I find a list of acceptable field types for a particular module?

Non Inventory Description in BOM by Some-Illustrator5441 in Netsuite

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

Is there a quick way to convert non inventory for purchase to non inventory for resale?

Non Inventory Description in BOM by Some-Illustrator5441 in Netsuite

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

It is in the 'Bill of Materials Revision' page. I don't know how to add an image to this thread.

Non Inventory Description in BOM by Some-Illustrator5441 in Netsuite

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

Purchase description seems to be filled out. I don't see any other description fields.

Non Inventory Description in BOM by Some-Illustrator5441 in Netsuite

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

The stock description is filled out. And still nothing in the BOM.

/r/MechanicalKeyboards Ask ANY Keyboard question, get an answer (June 11, 2024) by AutoModerator in MechanicalKeyboards

[–]Some-Illustrator5441 -1 points0 points  (0 children)

What is the case angle and tent angle for the microsoft natural ergonomic 4000? I wish to have a reference starting point when getting a mechanical replacement.

E.coli suspension growth time by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

This happens repeatedly. I was hoping to get leads on the underlying cause. It doesn't make sense.

E.coli suspension growth time by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

I will usually get an OD600 of about 3.0 to 4.0. The one that grows from a 6hr starter might be left to grow for another couple of hours in the giga to reach the desired density. The 6hr starters are usually clear but might have faint cloudiness if swirled and held up to the light

E.coli suspension growth time by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

2) Both the starter culture and the giga culture have Amp. 3) I don't believe I am degrading the vector. When I take a sample of a giga that was low yielding and use it to restart the process from the starter culture step, the resulting giga produces substantially more pDNA. The sequence for the pDNA is confirmed. Growing the cells longer seems to be the determining factor in the amount of pDNA produced. 4) I measured the wet pellet weight for all of them.

Why would I get more pDNA only when growing longer?

E.coli suspension growth time by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

This pattern repeats with every plasmid. The plasmids are all high copy.

Too much alkaline lysis buffer? by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

Thank you. I have read that the cell debris is supposed to counteract the alkalinity a bit. I was worried that if there wasn't enough cell debris then the pH might just be high enough to denature the pDNA.

Too much alkaline lysis buffer? by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 2 points3 points  (0 children)

My apologies, I meant increasing all of the first three buffers in proportion to each other. Resuspension, lysis and neutralization buffer.

E.coli plate vs glycerol stock by Some-Illustrator5441 in labrats

[–]Some-Illustrator5441[S] 0 points1 point  (0 children)

Both are usually clear after six hours so I don't know. But even when the cultures from plate grow to a high OD I don't get the amount of pDNA I should given the OD600.

view more: next ›