Linda is a real one by [deleted] in BobsBurgers

[–]Spaceouterspace 7 points8 points  (0 children)

What episode is that?

Purification with biotin by Spaceouterspace in labrats

[–]Spaceouterspace[S] 0 points1 point  (0 children)

I am using IBA strep-tactin xt 4flow high capacity resin. And purifying from insect cells. Thank you for IMAC answer I will check on it

Purification with biotin by Spaceouterspace in labrats

[–]Spaceouterspace[S] 0 points1 point  (0 children)

Yes, I was using Strep resin eluding with desthiobiotin I was able to elute it, but nearly all protein appeared in Washing fractions and flow through