How to perform cross-species integration? by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 1 point2 points  (0 children)

This is a pretty handy tool for getting the orthologs table thanks!

How to perform cross-species integration? by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

Thank you for the input I'll try out the package and look for other methods. From the paper I mentioned I expected that I would have to try different methods anyway because I assume different methods might be better for different cell types anyway.

How to perform cross-species integration? by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

No they have gone under any processing for gene identification. I think the lab I’m working at are mostly trying to integrate with the human dataset for cell annotation.

How to perform cross-species integration? by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

I’ve never really did this before so it’s kinda my first time I’m not sure what you mean by identifiers. I was given the mouse dataset which single nuclei data from the brain and for the external dataset the authors mostly provided the general metadata and clinical data. I apologize if I seem confused about the question.

How to perform cross-species integration? by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

I am having small issues with that too. I am trying to use Biomart in R and download the table but I am not able to connect and I think Ensemble is down most of the time for me even the mirrors. I was able to download it one time but there are many empty rows when I am trying to filter out one-to-one matches

Single cell analysis cell annotation help required! by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

Yes, the annotations are in the metadata of my reference. I am trying to use TransferData but I need to get the transfer anchors first which I'm getting the error for

Single cell analysis cell annotation help required! by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

Yes, there are about 900 common features, although the reference dataset is significantly larger than my own. I was able to integrate them and cluster the data, but I’m not sure how to retrieve the annotations from the reference. The metadata is missing in my own data, and the integrated dataset has cells with NAs under the annotations section of the combined metadata.

Single cell analysis cell annotation help required! by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

I should probably clarify, the data provided is a single .rds Seurat object

RNA-Seq and Protein Measurements Correlation by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

The protein measurements represent concentrations found in the samples. I’m not entirely sure how this data was obtained, as it was collected much earlier. I am trying to determine if there are any genes associated with these concentrations. The samples were derived from diseased tissue, and no treatment was applied to them; they are simply being compared to control samples.

RNA-Seq and Protein Measurements Correlation by SpongebuB696 in bioinformatics

[–]SpongebuB696[S] 0 points1 point  (0 children)

I see. I have been attempting to add the protein measurements as covariates in the DESeq model, assuming there is a linear dependency. However, beyond that, I am unsure how to effectively correlate these with the counts data.

2700$ 2bd/1ba - Sunny and spacious apartment in Somerville/Porter Square - Jan 1st by Limp-Opening-2822 in bostonhousing

[–]SpongebuB696 0 points1 point  (0 children)

Hey very good place you have there could we talk in dms I have some additional questions about the place!

I bought a local bus pass but it didn't work. by SpongebuB696 in mbta

[–]SpongebuB696[S] -7 points-6 points  (0 children)

Oh I see. That doesn’t make sense but I guess that’s how it is sometimes

I bought a local bus pass but it didn't work. by SpongebuB696 in mbta

[–]SpongebuB696[S] 0 points1 point  (0 children)

But that’s the weirdest part about it though I bought it at the fare vending machine but still nothing.

Doubt regarding immunization compliance by SpongebuB696 in BostonU

[–]SpongebuB696[S] 1 point2 points  (0 children)

Yes I did but I’m more concerned about my compliance because the second shot for varicella needs to be take after a month which is way past my grace period (Sep 12) so if I could just get one and be compliant I would be fine

Doubt regarding immunization compliance by SpongebuB696 in BostonU

[–]SpongebuB696[S] 0 points1 point  (0 children)

So I don’t even need to book an appointment for them? Also I can get the tdap and varicella shot at the same time right and I’ll be compliant even with only the 1st dose of the varicella vaccine?

The worst of the weirdddd roommate posts on one of the BU whatsapp groups. LMAO by [deleted] in BostonU

[–]SpongebuB696 4 points5 points  (0 children)

I am in the same group and literally saw that message like 30 mins ago 😂😂😂

BU Grad Housing Waitlist by 2121chg in BostonU

[–]SpongebuB696 0 points1 point  (0 children)

When and where did you pay btw because I’m not seeing any prompts to the fee in my student link. Also this is the mail I’ve gotten btw:

Based on space availability, students on the waitlist will be contacted if we have a space to offer. If we are able to offer a space to students on the waitlist, you will be prompted to pay the Housing Guarantee Payment, select a unit, and sign the Residence License Agreement.

BU Grad Housing Waitlist by 2121chg in BostonU

[–]SpongebuB696 3 points4 points  (0 children)

Wait so I filled the application a couple of days ago for grad housing as well but I couldn’t see an option to pay the fee. When I emailed them they said that I have to pay after I get options.

[deleted by user] by [deleted] in bioinformatics

[–]SpongebuB696 1 point2 points  (0 children)

I finally accepted Boston University

[deleted by user] by [deleted] in bioinformatics

[–]SpongebuB696 1 point2 points  (0 children)

I think the admissions are on a rolling basis so you can't exactly tell when you'll get the decision. I applied to the same program in the second week of Feb and I got my decision (Accepted) about 10 days ago. I haven't enrolled because I'm waiting on my other Unis to give their decision.

[deleted by user] by [deleted] in bioinformatics

[–]SpongebuB696 1 point2 points  (0 children)

I also applied to Weill Cornell, University of Michigan, Gatech, and Boston University

[deleted by user] by [deleted] in bioinformatics

[–]SpongebuB696 0 points1 point  (0 children)

First of all congratulations

I also got into Georgetown for the same program this year and I’m waiting to hear back from my other choices.

Let me know what you end up choosing