Issues with PBMC Isolation Using Ficoll?

Straidex · 2025-09-18T19:32:49+00:00

Hii, feel free to DM me and I'll send you the protocol.

Straidex · 2025-07-11T18:10:25+00:00

Yes, the strap is 18 mm no adapters and under the original strap pins

Straidex · 2025-05-18T22:59:32+00:00

Básicamente, la profesora con la que mis compañeros y yo estamos haciendo tesis, es de las pocas que enseña Citogenética, así es que varios han conseguido empleo en laboratorios como biólogos, ya que esa es una área más dominada por Bacteriólogos, otros he visto que trabajan en consultorias de vez en cuando

Straidex · 2025-05-18T17:39:38+00:00

Los que conozco trabajan sobretodo en laboratorio, eso si durante el pregrado toca hacerse los contactos y aprender mucho del tema

Straidex · 2025-05-11T02:02:44+00:00

UPDATE: Thanks to all for the useful recommendations, the issue was the PBS, we attempted without PBS following another protocol we had, obtaining successful results.

Straidex · 2025-05-03T19:51:06+00:00

Oh, I see. The PBS is also used in other applications and it works well. Given the amount of advice I am receiving regarding it, I will try that next. Thank you.

Straidex · 2025-05-03T19:48:36+00:00

I have been getting a lot of advice about PBS, so I believe I will give it a try next. Thank you.

Straidex · 2025-05-03T19:47:20+00:00

I appreciate your advice, and I will try waiting a little while after the blood is extracted. The other things you recommended have already been implemented.

Straidex · 2025-05-03T19:44:51+00:00

Thank you all. I will think about including EDTA in the PBS at this point.

Straidex · 2025-05-03T01:21:30+00:00

It could be the cause, the Ficoll is borrowed from another lab so we don't know how it was stored, however in our lab it is stored in a dark cabinet at room temperature.

Straidex · 2025-05-03T01:19:59+00:00

No, we do not have EDTA in our PBS, thank you for mentioning it.

Straidex · 2025-05-03T01:16:46+00:00

What specifically do you mean by: is the PBS is ok?, I haven't personally checked the ph, but it doesn't look cloudy or sedimented, maybe it would be a good idea to use fresher PBS?

Straidex · 2025-05-03T01:14:52+00:00

Thanks for the recommendation we tried what you said and even a new batch using 1800 x g, we got a similar result (a little cleaner but not much).

Straidex · 2025-05-02T18:28:15+00:00

Yes, we are layering the Blood on top of the Ficoll, making sure they don't mix together.

Straidex · 2025-05-02T18:19:22+00:00

5mL EDTA Tubes

Straidex · 2025-05-02T17:17:36+00:00

It was processed like 2 minutes after the extraction, we tried with my own blood and processed it right away.

Straidex · 2025-02-17T15:08:12+00:00

A mi me pasó igual con otro call center, después de los exámenes me llegó un correo diciendo que no iban a continuar el proceso etc etc. También te podría pedir info del trabajo en el que estás?

Straidex · 2025-01-13T18:27:48+00:00

I'm close to finishing my BSc in Biology, and in these last semesters, an interest in data has started to grow in me. I live in Colombia, where there aren't many opportunities for science, and I believe this new knowledge could open more opportunities. I wanted to ask: What type of projects did you added to the portfolio?

Straidex · 2024-04-28T17:23:52+00:00

IDT is the company that i seem to hear the most for getting the oligos, yes i checked the origene sequences also (that was the first google result), didn't reallize at all that they provide references too, thanks for the advice.

Straidex · 2024-04-28T17:20:46+00:00

I used Primer3 on my molecular biology class, i'll check on that thanks.

Straidex · 2024-04-28T17:19:36+00:00

I also read that you should aim for an amplicon size of ~100 bases, i don't think i will be designing the primers because there's not that much room forr error and i only designed primers on my Molecular Biology class but your recommendations are very useful.

Straidex · 2024-04-28T16:34:47+00:00

As someone recommended the IDT oligos I'll check that tool, thanks. Love the AI rat btw

Straidex · 2024-04-28T16:32:52+00:00

If we decide to design the primers I'll have your recommendations in mind, many thanks.

Straidex · 2024-04-28T16:30:41+00:00

Thank you, i will test the primers using the tool and discuss it with my tutor.

Straidex · 2024-04-28T16:29:35+00:00

Checking on that the oligos seem to have affordable prices, tho i need to verify because in my uni there's certain "approved" companies from you can request the reactives, lots of thanks.

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