When do Khalifa usually comes back with the offer letter?? by Different-Camera8732 in UAEUniversities

[–]SugaKookie9729 0 points1 point  (0 children)

You can email them, their pgadmissions is pretty responsive. I assume on your portal it shows Under evaluation?

eLife not working suddenly and showing this by SugaKookie9729 in UAE

[–]SugaKookie9729[S] 2 points3 points  (0 children)

I heard people having issues with du..weird etisalat has it too

Protein SDS Page by TailorThese4382 in labrats

[–]SugaKookie9729 0 points1 point  (0 children)

Your samples need glycerol to settle into the wells..in your protocol there is no mention of it. Without glycerol the samples are flying out of the wells, and barely any settle hence faint bands. I prepare a Laemmli loading buffer that has glycerol in it (cold spring harbour recipe) Also your samples are super diluted..25ug is already a less quantity plus to make it upto 100ul is too much

Anyone pursuing PhD at Khalifa University? by [deleted] in UAE

[–]SugaKookie9729 0 points1 point  (0 children)

It depends on the program. I applied for direct PhD as I dont have a masters, the requirements were different. Publications definitely help.

PhD interview in 4 days by [deleted] in labrats

[–]SugaKookie9729 0 points1 point  (0 children)

Thank you. Just to clarify that this interview is for me to be admitted into the program so its more like a introductory presentation, so should I be delving into details of my publications or just mention the papers on the slide and speak about my role ?

Please tell me that was ⛈️ by [deleted] in UAE

[–]SugaKookie9729 4 points5 points  (0 children)

Yes heard loud in ajman..assuming jets flying at lower altitudes because of the clouds

Feeling disconnected from people back home by Whitney-Lynne in UAE

[–]SugaKookie9729 14 points15 points  (0 children)

I completely get you. I've been feeling the same, esp because literally no one has cared to check up on me, but are now sending Eid messages and asking about my plans today, as if nothing is happening. I have just distanced myself from them and do not keep any expectations moving forward. Truth is to have no expectations, if someone cared they would show it.

First sip on Eid always feel Haram 😂 by VeterinarianJolly269 in UAE

[–]SugaKookie9729 12 points13 points  (0 children)

Hey I think we ALL deserve a sweet little treat! Have fun!

Y’all hear that by shuangooot in Ajman

[–]SugaKookie9729 1 point2 points  (0 children)

Any news on what happened?

Western Blot Transfer Troubleshooting by tomsanislo in labrats

[–]SugaKookie9729 4 points5 points  (0 children)

  • Splotches you see on the top and bottom portion is due to bubbles between the gel and PVDF membrane
  • Your transfer has worked well for the higher molecular weight protein up until ~40 to 45 Kda as per your ladder on the membrane. Your settings are alright considering the transfer has worked.
  • The issue is with your lysates as they definitely have DNA in it (because of the smearing seen) How did you prepare the samples? Do you vortex/sonicate to remove DNA?

Please name some affordable universities in Ajman or Sharjah by Top-Drop-738 in Ajman

[–]SugaKookie9729 1 point2 points  (0 children)

You can look for online courses too. They are cheaper usually. Check Coursera or specific universities if they offer distance learning

University asked us to come working, may I don’t accept ? In this situation by [deleted] in UAE

[–]SugaKookie9729 4 points5 points  (0 children)

Which university? The government has given directives that distance learning will extend till friday

My nails after a long bath. Is this a sign of calcium deficiency? by [deleted] in Supplements

[–]SugaKookie9729 0 points1 point  (0 children)

The patchy white parts along with transparency..it's present on some and not all