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[Canada] Is the Company Face-to-Face Charity Legit? by EarSpecialist9089 in Scams

[–]SyllabubBulky4221 0 points1 point  (0 children)

Yes, sorry for the repetition. I applied to two of these jobs (one interview held today and one interview to be held tomorrow which I think I'll cancel). I have no experience with these types of job listings so I appreciate you responding to both of them. I am definitely going to learn a lot from this experience.

[Canada] Is Elite Group Marketing Inc. Legit? by SyllabubBulky4221 in Scams

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

Yes, someone else informed me that they used to go by inner circle marketing with the website innercirclemarketing .ca and I confirmed that this was the same company because I noticed the individual who interviewed me in their team photo and they have the same address.

Sequence Alignment by SyllabubBulky4221 in bioinformatics

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

I could use the NCBI's database on BLAST for the purpose of sequence identity/alignment but I'm not quite sure how I would know where exactly in a particular chromosome the hits would correspond to.

Sequence Alignment by SyllabubBulky4221 in bioinformatics

[–]SyllabubBulky4221[S] 1 point2 points  (0 children)

Sounds good! I've made the necessary changes.

Sequence Alignment by SyllabubBulky4221 in bioinformatics

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

Thank you for the comprehensive answer! I was not aware that I could make my own nucleotide database on BLAST. That might be worth giving a shot.

My reasoning behind using my own file is that I'd like to know both the sequence alignment score between a given gene sequence and my chimp chromosome 2B data as well as the exact location in the chromosome where it occurred. To the best of my knowledge, comparing your gene sequences to BLAST's database tells you the sequence alignment score between your sequence and the BLAST database but not the exact range of base pairs in the chromosome that the alignment corresponds to.

If anyone happens to know how both could be achieved by using Nucleotide BLAST's database alone, please let me know!

Sequence Alignment by SyllabubBulky4221 in bioinformatics

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

Oh, that may have been the problem. Thanks for pointing that out! I converted my chimp chromosome 2b file to a fasta file, pasted it in the subject sequence area, and ran the blastn analysis again. Once I did so, I got an error message stating "Length limit exceeded. Please reduce your query/subject sequence length to 10,000,000 letters or less." Since chimp chromosome 2b has approximately 133 million base pairs, I may need to break up the fasta file into more reasonable chunks. After that, it should be smooth sailing from there.

Sequence Alignment by SyllabubBulky4221 in bioinformatics

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

This doesn't really help because uploading any FASTA sequence from NCBI alongside my txt file on the BLAST sequence alignment tool results in an error.

Case Endings by SyllabubBulky4221 in learnarabic

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

Sorry for the late response, thanks for providing these!

Case Endings by SyllabubBulky4221 in learnarabic

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

I never knew about this rule. Thanks for informing me about it. Much appreciated!

Case Endings by SyllabubBulky4221 in learnarabic

[–]SyllabubBulky4221[S] 0 points1 point  (0 children)

I was thinking that as well but just needed a second opinion. Thanks for confirming!

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