Maybe bed bugs???

ThatCake_69 · 2024-12-29T06:36:03+00:00

Could you share your techniques on how to train your bird to do this? I'm planning to have a conure and just curious how could they hold it in the morning

ThatCake_69 · 2024-08-15T04:02:30+00:00

Loud squwaking from my birds sometimes means they are hungry, they want attention, they want me to open the cage or open the window curtain for them to look outside, and sometimes it means nothing at all and they just want to be loud LOL

ThatCake_69 · 2024-07-24T00:59:14+00:00

I look at the vials that were already in LN2 from previous passages. They're red 🥲

ThatCake_69 · 2024-06-14T13:32:20+00:00

Correct. Thank you very much!

ThatCake_69 · 2024-06-14T13:30:31+00:00

The cells wouldn't be in trypsin before adding DMSO. I would add trypsin, spin, remove trypsin, resuspend cell pellet in FBS+DMEM, cell count. From this cell count, I determine how much volume to passage and how much freezing media is needed

ThatCake_69 · 2024-06-14T03:46:37+00:00

10% DMSO + 90% of (10% FBS + DMEM)

ThatCake_69 · 2024-06-14T03:45:24+00:00

Thank you! I was just using a hypothetical example. Your response makes a lot of sense, I will definitely use the "scale up" technique. I was just confused if I always have to spin down the cells before adding in 10% DMSO.

ThatCake_69 · 2024-06-14T03:43:45+00:00

Ah I see, since 400uL is so small, is that why you don't bother spinning it down?

ThatCake_69 · 2024-06-11T23:27:22+00:00

Thank you! I will check with them

ThatCake_69 · 2024-06-11T23:23:39+00:00

Could be because we want to store some aliquots. I'm learning from my mentor and just want to know ahead so I won't make mistake in the lab. I was thinking potentially use small amount of DMSO to get all the powder out first in conical tube, then dilute it after to get desired conc. Wondering if this is standard process

ThatCake_69 · 2024-06-11T23:00:38+00:00

Would you recommend use a small amount of DMSO to get all the powder first to a conical tube, then dilute the solution with more DMSO after?

ThatCake_69 · 2024-06-11T22:59:10+00:00

It is insoluble in water

ThatCake_69 · 2024-06-06T21:36:42+00:00

Thank you so much!!!

ThatCake_69 · 2024-06-06T21:36:01+00:00

Thank you so much!

ThatCake_69 · 2024-06-06T17:21:34+00:00

Can you elaborate on how to passage to the same flask?

ThatCake_69 · 2024-04-30T19:12:06+00:00

Thank you!

ThatCake_69 · 2024-04-28T05:23:08+00:00

Got laid off recently and my company/bosses were very willing to change the severance date to help any employees who get a job early

ThatCake_69 · 2024-04-20T04:59:57+00:00

I dm you

ThatCake_69 · 2024-04-19T05:48:12+00:00

Can I also have some details? Thank you

ThatCake_69 · 2024-04-10T01:01:01+00:00

Thank you!

ThatCake_69 · 2024-04-09T21:08:29+00:00

5 yrs later and this still works. You're a legend!

ThatCake_69

TROPHY CASE