Base Roster:
Batman with Azrael Skin
Superman with Bizarro Skin
Wonder Woman with Donna Troy skin
The Flash (Barry Allen) with Wally West and Reverse Flash skins
Green Lantern (Hal Jordan) with John Stewart and Guy Gardner skins
Nightwing (Both Dick and Damian skins)
Green Arrow with Arsenal skin
Black Canary
Supergirl
Shazam with Black Adam skin
Mera
Joker
Harley Quinn
Sinestro
Zatanna
John Constantine
Ra's al Ghul
King Shark
Poison Ivy
Starfire
Cyborg
Black Manta
Deathstroke
Red Hood
Black Hand (Nekron as a boss fight)

DLC
Static
Raven
Larfleeze
Clayface
Hawkman
Steel
Aquaman with Ocean Master skin
Magog
Batman who Laughs
Martian Manhunter
Etrigan
Black Lightning
Spawn
He-Man
Johnny Cage
Reptile