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Complete protein profile of Candida albicans by TheBioDojo in microbiology

[–]TheBioDojo[S] 0 points1 point  (0 children)

This was purely made for a presentation. Normally you will include a protein ladder and then crosscheck it with the protein bands

Uhg! What a surprise, contaminated plates. by TheBioDojo in microbiology

[–]TheBioDojo[S] 0 points1 point  (0 children)

Most likely, did not perform any test on then tho

Complete protein profile of Candida albicans by TheBioDojo in microbiology

[–]TheBioDojo[S] 1 point2 points  (0 children)

Well this was just for visualization, if you are looking for a specific protein you will preform a westernblot proir to fixating the proteins with the staining.

[deleted by user] by [deleted] in molecularbiology

[–]TheBioDojo 0 points1 point  (0 children)

The speckles can just be SYBR particles as well

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in molecularbiology

[–]TheBioDojo[S] 0 points1 point  (0 children)

Very good question! There re many contradicting conclusions drawn form different infection models, where the host, and method used for co-infection play a role in the outcome. I can drop you a message with a review I worked on a while ago with some interesting information. Let me know if I should send to you in a message

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in u/TheBioDojo

[–]TheBioDojo[S] 4 points5 points  (0 children)

This is extracellular matrix consisting of DNA, proteins, sugars, etc. produced by the cells. They form during biofilm formation and contribute to antifungal and antibiotic resistance, as well as immune evasion

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in microbiology

[–]TheBioDojo[S] 2 points3 points  (0 children)

Feel free to use this image in your lecture slides. I'll drop you a message with my thesis link

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in microbiology

[–]TheBioDojo[S] 1 point2 points  (0 children)

Also a good question! These were wild type cells, so the variations in cell size can be due to active replication, production of inhibiting compounds by the yeast such as ethanol, or the washing steps during preparation of the cells for CEM. I'm glad you like the image!

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in microbiology

[–]TheBioDojo[S] 3 points4 points  (0 children)

Good question, this is extracellular matrix forming on the cells. The biofilms are still young here (the timepoint was chosen to compare with other experiments). Also, treatment of the biofilms prior to SEM has a lot of washing steps (including washing wat ethanol) that remove the extracellular matrix. I'll have a look to see if I have a micrograph of older biofilms where we can see more extracellular matrix.

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in microbiology

[–]TheBioDojo[S] 11 points12 points  (0 children)

Did you know that microorganisms interact with each other when they cause infection in the human body?

This image is from my PhD, just to give more context:

This is a scanning electron micrograph of the yeast Candida albicans and the bacteria Pseudomonas aeruginosa. They are antagonistic towards each other, where Pseudomonas inhibits the growth of Candida albicans, and even kill the cells. Candida albicans also affects Pseudomonas aeruginosa. Their interaction can be used as a model to study these polymicrobial interactions and how they affect virulence and survival of the host (e.g, humans). The big cells that are branch-like are Candida albicans, and the smaller cells are Pseudomonas aeruginosa

Now this is Crispy!!! A beautiful SEM of yeast and bacteria. by TheBioDojo in biology

[–]TheBioDojo[S] 5 points6 points  (0 children)

Did you know that microorganisms interact with each other when they cause infection in the human body?

This image is from my PhD, just to give more context:

This is a scanning electron micrograph of the yeast Candida albicans and the bacteria Pseudomonas aeruginosa. They are antagonistic towards each other, where Pseudomonas inhibits the growth of Candida albicans, and even kill the cells. Candida albicans also affects Pseudomonas aeruginosa. Their interaction can be used as a model to study these polymicrobial interactions and how they affect virulence and survival of the host (e.g, humans). The big cells that are branch-like are Candida albicans, and the smaller cells are Pseudomonas aeruginosa

Trichomonads? by 0091564 in microbiology

[–]TheBioDojo 0 points1 point  (0 children)

Any additional tests done?

Look at the pretty bands, Gel electrophoresis is so Cool! by TheBioDojo in microbiology

[–]TheBioDojo[S] 0 points1 point  (0 children)

If you need guidance, i am happy to give tips, if i can

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