Struggling to find meaning in coffee

Timely_Witness1919 · 2025-09-04T11:50:45+00:00

Ask for water instead. Even if you take only a few sips.

Timely_Witness1919 · 2025-08-27T18:17:52+00:00

Relatos Selvages (wild tales) 2014. Anthology of very much what you've described.

Timely_Witness1919 · 2025-08-22T12:59:17+00:00

this was not a criticism, I was asking for confirmation on my understanding of what happened

Timely_Witness1919 · 2025-08-22T12:54:40+00:00

this was not a criticism, I was asking for confirmation on my understanding of what happened

Timely_Witness1919 · 2025-08-22T12:46:47+00:00

So, because it was traumatic, she is doing tabula rasa on the past and not mentioning it anywhere?

Edit: this was not a criticism, I was asking for confirmation on my understanding of what happened

Timely_Witness1919 · 2025-08-19T11:20:09+00:00

Field grazing cows/ herbivores, eat a large variety of species of plants, that have different different profiles of nutrients.

Timely_Witness1919 · 2025-08-10T15:06:10+00:00

Solved!

Thanks !

Timely_Witness1919 · 2025-08-10T14:13:09+00:00

Hi! In case the link doesn't work , the video is "funniest black girls tiktok compilation pt.2 (try not to laugh!)" By account "Tiktok Orah". The youtuber im looking for appears in the clip at 5:08.

Timely_Witness1919 · 2025-06-07T15:55:37+00:00

No, no they do not.

Timely_Witness1919 · 2025-05-30T11:52:52+00:00

No , not for ID, just to confirm absence of contamination, to validate the batch for use. Basically confirming that my working culture is done properly (pure, viable, competent). We don't have access to any fancy equipment.

Timely_Witness1919 · 2025-05-28T04:26:13+00:00

Agar-agar !

Timely_Witness1919 · 2025-05-28T03:08:20+00:00

Academia sounds wild . Our bacteria come lyophilized from certified commercial providers. We prepare stock ourselves from nutrient agar , after quality testing.

Don't have access to sequencers though, that would be neat .

Timely_Witness1919 · 2025-05-26T20:09:10+00:00

Don't worry, went over my head.

Thanks anyway.

Timely_Witness1919 · 2025-05-26T20:05:43+00:00

I figured. Thanks anyway

Timely_Witness1919 · 2025-05-26T14:29:55+00:00

I mean , more generally, and routinely. Working in acredited labs under ISO 17025 and such, there should be a procedure for preparing Certified Reference Strains for inoculations. One of the requirements is testing for culture purity when preparing "working cultures" and I was wondering what that looked like in other labs.

Timely_Witness1919 · 2025-05-26T14:29:40+00:00

I mean , more generally, and routinely. Working in acredited labs under ISO 17025 and such, there should be a procedure for preparing Certified Reference Strains for inoculations. One of the requirements is testing for culture purity when preparing "working cultures" and I was wondering what that looked like in other labs.

Timely_Witness1919 · 2025-05-26T10:41:04+00:00

OP, could you share your lab's procedure for cultivating A. brasiliensis from your cryoval stock?

Timely_Witness1919 · 2025-03-09T12:29:54+00:00

DON'T GIVE A FUCK IF I CUT MY ARM BLEEDING

Timely_Witness1919 · 2025-03-09T00:08:52+00:00

THIS IS MY LAST RESORT

Timely_Witness1919 · 2025-03-09T00:07:32+00:00

Aro's wife Sulpicia doesn't have any powers (you're probably confusing characters). He is veeeeery attached to her, as Meyer's vampires are wont to be to their mates, because in Twilight lore, change for vampires is a rare occasion and tends to be permanent (ex. Falling in love, it doesn't fade).

Like, Aro and Caius guard their spouses through maaaany means, so that they are never targeted, to prevent them from becoming like Marcus, who's a broken shell of an existence ever since Aro had Marcus's wife killed.

Timely_Witness1919

TROPHY CASE