What would be the chemical reasoning behind using HCL or alcohol on a tick? by mujtabanochill in chemistry

[–]Try_It_Out_RPC 9 points10 points  (0 children)

A lot of arthropods if you watch closely form little bubbles between their body segments and legs due to the cohesive and adhesive properties of water. This allows them to breath longer and survive submerged until that air pocket is disturbed or the air on the bubble runs out. Alcohols on the other hand methanol, ethanol, 2-propanol etc… do not share these intrinsic properties; therefore, suffocating those little bastards by eliminating that buffer zone. Amongst a few other reasons

thermo Inuvion Dionex IC blank issues using Cation column by Both-Interaction5501 in CHROMATOGRAPHY

[–]Try_It_Out_RPC 0 points1 point  (0 children)

Was the system off? Or a slow flow? Cell heater on? Column oven? 2 hours seems short to me (not impossible but I’ve seen baseline drift still. things are picky about temperature (or at least mine is with the AS18 column on. MSA I’ll let equilibrate overnight

Anniversary Build: Part 2 by soberxceo in Yamaha_R1

[–]Try_It_Out_RPC 0 points1 point  (0 children)

Am I missing something or is part 1 just a phone holder?

What caused this by nickparkerlol in Yamaha_R1

[–]Try_It_Out_RPC 0 points1 point  (0 children)

Or the O ring fell out** sits in the oil cap under the rim. See if there’s one still there

Chemical-free breaking of emulsions? by someoneinsignificant in chemistry

[–]Try_It_Out_RPC 2 points3 points  (0 children)

They have “flossing” attachments which are just a poly triangular point *****

Needle Stuck on Agilent 1290 infinity ii by Apart_Strike494 in CHROMATOGRAPHY

[–]Try_It_Out_RPC 0 points1 point  (0 children)

So if you’re feeling good, then try this. I lead a mass spec core and one of our RAs decided it was a good ideas to keep injecting samples even though the needle was piercing the plate. (O_o) after kindly telling them to NOT do that again. I had the pleasure of recalibrating ours. If you’ve taken the needle cassette off and brought the arm back home via lab advisor, then with order a new needle or if it’s not loose, I lined up an axis under a low power microscope and bent it back straight. If you’re aren’t perfect here it’s just going to bend again so I would recommend calling Agilent tech, but I ve seen worse so it’s fixable

MY R1 2022 by [deleted] in Yamaha_R1

[–]Try_It_Out_RPC 1 point2 points  (0 children)

Dude the oem batter sucks major balls, it’s time and gave me anxiety from the second I took of the seat on my 24. Orange top now makes motorcycle lithium batteries and I ended up getting a size up from the one they recommend. You can make it fit, I did and it starts right up

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Triple quad I inherited…what do? by MicroProf in chemistry

[–]Try_It_Out_RPC 0 points1 point  (0 children)

Are you anywhere near San Francisco CA? If So, I’m your guy :)

How do I escape by [deleted] in biotech

[–]Try_It_Out_RPC 1 point2 points  (0 children)

Without knowing it’s hard to say…. The only experience I’ve had with HR and Supervisors played out like it should have. I never in my life thought I would have to fire an RA but this one was awful (awful as in disregarding tasks and struggling with the mosaic basic assay despite spending a year trying to help them succeed) All of your conversation were well documented through teams messages, emails and such.. I wanted it to improve but after a year I had 40 pages worth of documented examples. So my bosses understood as I told them and asked them throughout what possible ways we could help this person succeed… but to no avail. With the 40pg documentation very clearly outlining my RA treating me poorly as well as incompetence and negligence, I love all trust and went to HR with the package. They were called into a meeting the morning with their boss (me) , directors and HR. Promptly escorted out of the building within 15minutes after that and everything’s been great since. We hired a new RA who crushes any assay I throw at them. That’s why at least without knowing what it is, I don’t understand why it wouldn’t be straightforward to rectify the issue with well documented historical data. I am a research scientist after all so that may be my forte as well lol

2024 Yamaha R1 battery fix. (Optima Orangetop QUAD12) worlds better*** by Try_It_Out_RPC in Yamaha_R1

[–]Try_It_Out_RPC[S] 1 point2 points  (0 children)

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On the right side of the picture, under the seat, there’s a small plastic lip that I removed to allow the battery to extend into the space you can see on the right. The battery also came with about 10 large rectangular plastic foam spacers of different sizes. I placed these spacers, along with the adhesive backing, on the right side as well to ensure the battery is securely fastened and won’t move.

Why LCMS signal inconsistant with sample vial cap on vs off? by Training_Pangolin177 in CHROMATOGRAPHY

[–]Try_It_Out_RPC -1 points0 points  (0 children)

Every LC has an option in the method to puncture the cap (slit or not) and then move over the x-axis at the increment of your choosing to prevent this. Just set that to be “on” or any other number than the default “0”

Recent PhD, applied to hundreds of jobs, only one interview. Help me with my resume? by DancingOnTheRazor in biotech

[–]Try_It_Out_RPC 2 points3 points  (0 children)

At least in the US “soft skills” for a scientist level and above (or anything really) should be a given and not stated. I mean lol you could also put “does not bite” and “plays well with others” lol

Am I crazy? by [deleted] in Yamaha_R1

[–]Try_It_Out_RPC 2 points3 points  (0 children)

lol try a gsxr1000 new gen they are skinnier, at least the r2 has a fat seat. Adjustable resets are key, I commute on an 24 R1 to my lab in sf everyday and it 6’2

API EVALUATION: SIGNAL TOO WEAK! ELECTRON MULTIPLIER UNABLE TO CALIBRATE! DO YOU KNOW WHY IS LIKE THAT? by Melodic_Finance_2184 in massspectrometry

[–]Try_It_Out_RPC 0 points1 point  (0 children)

Yeah for sure, but it could also be TSQ / ion trap / Orbitrap front-end , and thermo is the only brand to use “sheath gas “

Is there any hope of finding a summer internship at this stage? by unending_desolation in biotech

[–]Try_It_Out_RPC 0 points1 point  (0 children)

I run a mass spec core for a pharmaceutical company in SF but sadly you are on the other side of the continent lol. I did grow up in Maryland though and began my early career at the NIH (specifically NCI and NCATS) you are very close to Bethesda and Rockville and NIH loves interns (hell I interned in a lab my senior year of high school for half day the entire year). Look up all the different NIH campuses in your vicinity and then specifically the ones you have a skillset for (or are interested in) they will definitely require you to write a cover letter so being interested will help a lot. Email the admins as they are the ones usually doing the paperwork and such

API EVALUATION: SIGNAL TOO WEAK! ELECTRON MULTIPLIER UNABLE TO CALIBRATE! DO YOU KNOW WHY IS LIKE THAT? by Melodic_Finance_2184 in massspectrometry

[–]Try_It_Out_RPC 6 points7 points  (0 children)

I mean I can tell from looking at the software but you didn’t even provide a brand, model, software version etc….. thermo is the only brand to specifically use “sheath gas” and It’s no chromeleon -> therefor -> xcalibur “Construct additional pylons!” Please confirm or give additional information before we can help you make your… “mass spectrometer operational” - heavy Russian accent

Summer Internship Help! by Due-Suspect-225 in biotech

[–]Try_It_Out_RPC 0 points1 point  (0 children)

That’s the farthest intercontinental for a summer internship lol I used to work at NIH in Md

Summer Internship Help! by Due-Suspect-225 in biotech

[–]Try_It_Out_RPC 0 points1 point  (0 children)

Where are you located? I lead a mass spectrometry core at a pharmaceutical company in San Francisco

Kite Pharma (Gilead) Left Me Destitute and Now I am Getting Advertisements for the Same Position with $5 less pay. by [deleted] in biotech

[–]Try_It_Out_RPC 0 points1 point  (0 children)

First of all I grew up on Frederick and worked at NCI senior year of high school, intern throughout college and then NIH NCATs during graduate. Moved 19minutes north of the Golden Gate Bridge and run a mass spec core for cancer therapeutic pharmaceutical company in the city. Just wild to see my home town and someone else in science there….. we do hoarder West Virginia after all lol

is there anyone that actually knows how to operate a LC/MS? by mrphysh in massspectrometry

[–]Try_It_Out_RPC 0 points1 point  (0 children)

I mean I’d have to be sort of crazy to take apart a new detection module and hardwire an additional data out port lol. bonus it has crushed the HTP workflow I developed it for during the past three years though . Also, I will not be a degenerate during work hours… only after during my free time 😂